Fitness data for MMJJ_RS06750 in Methanococcus maripaludis JJ

homoaconitase large subunit
SEED: Coenzyme B synthesis from 2-oxoglutarate: steps 4, 7, 8, 11, and 12 [large subunit]
KEGG: homoaconitate hydratase

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 3.8)


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group condition fitness t score  
attachment assay; attached sample McCas-formate glass attached -5.2 -1.7 compare
attachment assay; attached sample McCas-formate glass attached -5.2 -1.9 compare
attachment assay; attached sample McCas-formate glass attached -5.2 -1.9 compare
nutrient t1 formate McAA thiamine dropout -5.0 -2.8 compare
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -4.6 -2.7 compare
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -4.5 -2.5 compare
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -4.5 -2.5 compare
iron control, 1x iron with casaminos and vitamins, H2 media -4.5 -2.7 compare
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -4.5 -2.3 compare
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE -4.4 -1.9 compare
iron Iron limitation (0.2x), formate media -4.2 -3.2 compare
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -4.2 -1.7 compare
rich t1 H2 McCas, 5g/L bicarb, 40 psi H2CO2 -4.2 -4.0 compare
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -4.2 -2.1 compare
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -4.1 -3.3 compare
hydrogen no stress control H2 no stress control -4.0 -4.0 compare
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 -3.9 -1.4 compare
hydrogen no stress control H2 no stress control -3.9 -4.5 compare
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -3.8 -3.0 compare
nutrient t1 formate McAA pyroxidine HCl dropout -3.8 -3.2 compare