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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
Pf6N2E2_4178 and Pf6N2E2_4179 are separated by 150 nucleotides
Pf6N2E2_4179 and Pf6N2E2_4180 are separated by 208 nucleotides
Pf6N2E2_4180 and Pf6N2E2_4181 are separated by 491 nucleotides
Pf6N2E2_4181 and Pf6N2E2_4182 are separated by 33 nucleotides
Pf6N2E2_4178 - Glutathione S-transferase (EC 2.5.1.18), at 4,862,881 to 4,863,576
_4178
Pf6N2E2_4179 - Transcriptional regulator, TetR family, at 4,863,727 to 4,864,290
_4179
Pf6N2E2_4180 - FIG00955126: hypothetical protein, at 4,864,499 to 4,865,623
_4180
Pf6N2E2_4181 - Organic hydroperoxide resistance protein, at 4,866,115 to 4,866,534
_4181
Pf6N2E2_4182 - Cell division transporter, ATP-binding protein FtsE (TC 3.A.5.1.1), at 4,866,568 to 4,866,936
_4182
Group
Condition
Pf6N2E2
_4178
Pf6N2E2
_4179
Pf6N2E2
_4180
Pf6N2E2
_4181
Pf6N2E2
_4182
motility
inner cut, LB soft agar motility assay
-0.4
-0.3
+0.7
-4.9
-0.0
motility
inner cut, LB soft agar motility assay
-0.4
-0.7
+0.6
-4.6
+0.2
stress
deoxycholate 0.0125 M
+0.1
+0.2
-0.5
-4.1
-0.2
stress
Bile salts 0.0125 M
-0.0
-0.1
+0.2
-4.2
-0.2
stress
periodate 0.00125 M
-0.0
-0.2
-0.1
-3.7
+0.2
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C
+0.0
+0.0
-0.0
-2.9
-0.2
stress
PTLB purified from Pseudomonas sp. FW300-N2E2 0.05 x
+0.0
-0.0
-0.3
-2.4
-0.0
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C
-0.2
+0.2
-0.1
-2.7
+0.1
stress
Doxycycline hyclate 0.0001 mg/ml
-0.2
-0.0
-0.1
-2.1
+0.1
stress
Hydroxide 0.05 M
-0.4
-0.3
-0.1
-1.4
-0.1
stress
molybdate 0.25 M
-0.1
-0.1
+0.0
-2.4
+0.4
carbon source
Ying_AminoAcid20 rep B; time point 2
-0.1
+0.0
-0.2
-1.3
-0.6
lb
LB
-0.1
+0.1
-0.1
-1.9
-0.2
carbon source
Ying_Nucleobase14 rep A; time point 2
+0.2
-0.0
+0.0
-1.2
-0.8
carbon source
Ying_AminoAcid20 rep A; time point 2
-0.3
+0.3
+0.2
-1.3
-0.6
motility
outer cut, LB soft agar motility assay
-0.3
-0.7
+0.5
-1.6
+0.4
lb
LB no stress 48 hours
-0.3
+0.2
-0.3
-1.4
+0.2
carbon source
Ying_sugar7 rep B; time point 1
-0.2
+0.2
+0.3
-1.1
-0.7
motility
inner cut, LB soft agar motility assay
-0.1
-0.6
+0.5
-1.0
+0.4
vitamin
No vitamins t2
-0.2
-0.3
-0.4
+1.5
-0.6
motility
outer cut, LB soft agar motility assay
-0.8
-0.6
+0.3
+1.0
+0.6
vitamin
With vitamins t1
-0.1
-0.0
-0.2
+1.4
-0.5
stress
Zinc chloride 0.0000015625 M
-0.1
-0.1
-0.1
+1.6
-0.8
nitrogen source
L-Serine (N)
-0.3
-0.3
+0.1
+1.2
+0.2
nitrogen source
Cytidine (N)
-0.0
-0.1
+0.1
+1.6
-0.5
carbon source
Ying_AminoAcid20 rep A; time point 3
-0.2
+0.1
-0.1
+1.5
-0.2
carbon source
Ying_Nucleobase14 rep A; time point 3
-0.3
+0.0
+0.1
+1.5
-0.2
stress
Hydroxylamine 0.5 M
-0.6
-0.0
+0.0
+1.8
+0.1
stress
Chloride 600 mM
-0.2
-0.2
-0.0
+1.6
+0.2
nitrogen source
Glycine (N)
+0.1
-0.1
-0.1
+2.1
-0.0
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