Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_4178 and Pf6N2E2_4179 are separated by 150 nucleotidesPf6N2E2_4179 and Pf6N2E2_4180 are separated by 208 nucleotidesPf6N2E2_4180 and Pf6N2E2_4181 are separated by 491 nucleotidesPf6N2E2_4181 and Pf6N2E2_4182 are separated by 33 nucleotides Pf6N2E2_4178 - Glutathione S-transferase (EC 2.5.1.18), at 4,862,881 to 4,863,576 _4178 Pf6N2E2_4179 - Transcriptional regulator, TetR family, at 4,863,727 to 4,864,290 _4179 Pf6N2E2_4180 - FIG00955126: hypothetical protein, at 4,864,499 to 4,865,623 _4180 Pf6N2E2_4181 - Organic hydroperoxide resistance protein, at 4,866,115 to 4,866,534 _4181 Pf6N2E2_4182 - Cell division transporter, ATP-binding protein FtsE (TC 3.A.5.1.1), at 4,866,568 to 4,866,936 _4182
Group Condition Pf6N2E2_4178 Pf6N2E2_4179 Pf6N2E2_4180 Pf6N2E2_4181 Pf6N2E2_4182
motility inner cut, LB soft agar motility assay -0.4 -0.3 +0.7 -4.9 -0.0
motility inner cut, LB soft agar motility assay -0.4 -0.7 +0.6 -4.6 +0.2
stress deoxycholate 0.0125 M +0.1 +0.2 -0.5 -4.1 -0.2
stress Bile salts 0.0125 M -0.0 -0.1 +0.2 -4.2 -0.2
stress periodate 0.00125 M -0.0 -0.2 -0.1 -3.7 +0.2
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C +0.0 +0.0 -0.0 -2.9 -0.2
stress PTLB purified from Pseudomonas sp. FW300-N2E2 0.05 x +0.0 -0.0 -0.3 -2.4 -0.0
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C -0.2 +0.2 -0.1 -2.7 +0.1
stress Doxycycline hyclate 0.0001 mg/ml -0.2 -0.0 -0.1 -2.1 +0.1
stress Hydroxide 0.05 M -0.4 -0.3 -0.1 -1.4 -0.1
stress molybdate 0.25 M -0.1 -0.1 +0.0 -2.4 +0.4
carbon source Ying_AminoAcid20 rep B; time point 2 -0.1 +0.0 -0.2 -1.3 -0.6
lb LB -0.1 +0.1 -0.1 -1.9 -0.2
carbon source Ying_Nucleobase14 rep A; time point 2 +0.2 -0.0 +0.0 -1.2 -0.8
carbon source Ying_AminoAcid20 rep A; time point 2 -0.3 +0.3 +0.2 -1.3 -0.6
motility outer cut, LB soft agar motility assay -0.3 -0.7 +0.5 -1.6 +0.4
lb LB no stress 48 hours -0.3 +0.2 -0.3 -1.4 +0.2
carbon source Ying_sugar7 rep B; time point 1 -0.2 +0.2 +0.3 -1.1 -0.7
motility inner cut, LB soft agar motility assay -0.1 -0.6 +0.5 -1.0 +0.4
vitamin No vitamins t2 -0.2 -0.3 -0.4 +1.5 -0.6
motility outer cut, LB soft agar motility assay -0.8 -0.6 +0.3 +1.0 +0.6
vitamin With vitamins t1 -0.1 -0.0 -0.2 +1.4 -0.5
stress Zinc chloride 0.0000015625 M -0.1 -0.1 -0.1 +1.6 -0.8
nitrogen source L-Serine (N) -0.3 -0.3 +0.1 +1.2 +0.2
nitrogen source Cytidine (N) -0.0 -0.1 +0.1 +1.6 -0.5
carbon source Ying_AminoAcid20 rep A; time point 3 -0.2 +0.1 -0.1 +1.5 -0.2
carbon source Ying_Nucleobase14 rep A; time point 3 -0.3 +0.0 +0.1 +1.5 -0.2
stress Hydroxylamine 0.5 M -0.6 -0.0 +0.0 +1.8 +0.1
stress Chloride 600 mM -0.2 -0.2 -0.0 +1.6 +0.2
nitrogen source Glycine (N) +0.1 -0.1 -0.1 +2.1 -0.0
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