Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_2450 and Pf6N2E2_2451 are separated by 54 nucleotidesPf6N2E2_2451 and Pf6N2E2_2452 are separated by 500 nucleotidesPf6N2E2_2452 and Pf6N2E2_2453 are separated by 139 nucleotidesPf6N2E2_2453 and Pf6N2E2_2454 are separated by 128 nucleotides Pf6N2E2_2450 - DEAD-box ATP-dependent RNA helicase CshA (EC 3.6.4.13), at 2,901,123 to 2,902,796 _2450 Pf6N2E2_2451 - hypothetical protein, at 2,902,851 to 2,903,003 _2451 Pf6N2E2_2452 - Spermidine synthase (EC 2.5.1.16), at 2,903,504 to 2,904,259 _2452 Pf6N2E2_2453 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase II (EC 2.5.1.54), at 2,904,399 to 2,905,748 _2453 Pf6N2E2_2454 - FIG074102: hypothetical protein, at 2,905,877 to 2,907,106 _2454
Group Condition Pf6N2E2_2450 Pf6N2E2_2451 Pf6N2E2_2452 Pf6N2E2_2453 Pf6N2E2_2454
carbon source L-Phenylalanine (C) N.D. N.D. -0.1 -1.0 -0.1
stress DEANONOate 0.000625 M N.D. N.D. -0.4 -0.5 -0.1
stress RCH2 defined with Cobalt chloride 0.02 mM N.D. N.D. -0.6 -0.2 -0.2
stress RCH2 defined with Cobalt chloride 0.04 mM N.D. N.D. -0.8 -0.0 -0.0
stress Chloramphenicol 0.008 mg/ml N.D. N.D. -0.7 -0.2 -0.0
carbon source L-Phenylalanine (C) N.D. N.D. -0.0 -0.8 -0.1
stress tungstate 0.05 M N.D. N.D. -0.4 -0.4 -0.0
stress phosphite 0.25 M N.D. N.D. -0.3 -0.4 -0.0
carbon source Ying_sugar7 rep B; time point 1 N.D. N.D. -0.4 -0.2 -0.2
lb LB no stress 48 hours N.D. N.D. -0.3 -0.2 -0.2
stress Fluorophosphate 0.125 M N.D. N.D. -0.5 -0.1 +0.0
stress dimethyl sulfone 0.25 M N.D. N.D. -0.5 -0.2 +0.0
stress RCH2 defined with Cobalt chloride 0.02 mM N.D. N.D. -0.7 +0.1 -0.0
stress RCH2 defined with Cobalt chloride 0.04 mM N.D. N.D. -0.7 +0.2 +0.0
stress Hydroxylamine 0.5 M N.D. N.D. +0.2 -0.8 +0.1
stress RCH2 defined with Nickel (II) chloride 0.04 mM N.D. N.D. -0.5 -0.2 +0.2
nitrogen source Glycine (N) N.D. N.D. +0.2 -0.3 -0.2
nitrogen source L-Arginine (N) N.D. N.D. -0.3 +0.3 -0.3
stress DEANONOate 0.000625 M N.D. N.D. +0.4 -0.5 -0.1
nitrogen source L-Alanine (N) N.D. N.D. +0.3 -0.5 +0.0
nitrogen source L-Serine (N) N.D. N.D. +0.3 -0.5 +0.1
stress deoxycholate 0.0125 M N.D. N.D. -0.2 +0.4 +0.1
resistance 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine N.D. N.D. +0.6 -0.1 -0.1
stress Cobalt chloride 0.4 mM N.D. N.D. +0.4 +0.2 -0.2
motility inner cut, LB soft agar motility assay N.D. N.D. -0.2 +0.3 +0.4
resistance 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine N.D. N.D. +0.7 +0.0 -0.1
resistance 20 mM putrescine N.D. N.D. +0.7 -0.0 -0.1
stress Cobalt chloride 0.6 mM N.D. N.D. +0.5 +0.5 -0.2
stress copper (II) chloride 3 mM N.D. N.D. +0.6 +0.1 +0.1
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine N.D. N.D. +0.9 +0.1 +0.0
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