Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2340 and PP_2341 are separated by 10 nucleotidesPP_2341 and PP_5519 are separated by 64 nucleotidesPP_5519 and PP_2342 are separated by 21 nucleotidesPP_2342 and PP_2343 overlap by 9 nucleotides PP_2340: PP_2340 - conserved protein of unknown function, at 2,672,687 to 2,673,205 _2340 PP_2341: PP_2341 - 6-carboxy-5,6,7,8-tetrahydropterin synthase, at 2,673,216 to 2,673,572 _2341 PP_5519: PP_5519 - conserved protein of unknown function of phage origin, at 2,673,637 to 2,673,810 _5519 PP_2342: PP_2342 - conserved protein of unknown function, at 2,673,832 to 2,674,026 _2342 PP_2343: PP_2343 - conserved protein of unknown function, at 2,674,018 to 2,674,749 _2343
Group Condition PP_2340 PP_2341 PP_5519 PP_2342 PP_2343
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.6 -7.5 -0.5 +0.2 -0.0
pH Growth at pH7 and (C) Trisodium citrate -0.5 -6.4 +0.1 -0.9 +0.2
nitrogen source Urea (N) +0.2 -6.7 -1.0 +0.5 -0.2
temperature shift Temperature shift 30_to_25; with MOPS -0.0 -6.4 +0.4 +0.1 -0.2
temperature shift Temperature shift 30_to_21; with MOPS -0.1 -2.3 -1.9 +0.0 -0.2
nitrogen source NAG (N); with MOPS +0.2 -4.3 +0.2 -0.2 -0.0
nitrogen source nitrite (N) -2.1 -1.0 -0.9 +0.1 +0.0
phage P. putida Missley MOI 0.1 -1.5 -2.2 +0.4 -0.3 -0.1
stress R2A with Fusaric 480 ug/mL +0.8 -4.0 +0.1 -0.0 -0.0
pH Growth at pH6 and (C) Trisodium citrate +0.4 -2.2 -0.8 -0.1 -0.2
nitrogen source Ammonium chloride (N); with MOPS -0.2 -3.0 -0.0 +0.3 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -2.3 -0.6 +0.1 +0.1
carbon source Valeric (C) -1.0 -0.9 +0.7 -0.8 -0.4
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.5 -3.1 -1.0 +0.8 +0.4
nitrogen source D-2-Aminobutyric (N) -0.2 -1.5 +0.4 -1.3 +0.3
no stress control Growth with no stress +0.6 -1.8 -0.9 -0.3 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -2.7 -0.0 +0.9 +0.3 -0.1
phage P. putida Sci small MOI 0.1 -2.9 +0.4 +0.3 +0.1 +0.5
pH Growth at pH9 and (C) Trisodium citrate +0.4 -1.9 -0.7 +0.6 +0.1
carbon source Valerolactone (C) +0.9 +0.5 -1.0 -1.2 +0.0
temperature Growth at 30C; with MOPS +0.5 -2.5 +1.0 +0.5 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.6 +0.8 -1.5 -0.7 +0.4
phage P. putida South MOI 1 -1.5 +1.5 -0.4 +0.1 -0.0
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.7 +1.3 -1.0 +0.4 -0.1
nitrogen source NAG (N); with MOPS +0.7 +0.8 -1.8 +0.3 -0.0
carbon source Crotonic (C) -1.7 +1.4 +0.2 +0.2 +0.2
carbon source Myristic (C) +0.9 +1.2 -1.8 +0.3 +0.1
micoeukaryotes Tetrahymena sp., Mixed culture-2 -0.3 +2.1 +0.4 -0.9 +0.0
nitrogen source Urea (N); with MOPS +0.8 +1.0 +0.4 +0.9 +0.4
carbon source carbon source 2-Deoxy-D-glucose 100 mM +0.7 +2.3 +0.1 +0.5 -0.1
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