Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_4657 and PP_4658 are separated by 171 nucleotidesPP_4658 and PP_4659 are separated by 122 nucleotidesPP_4659 and PP_4660 are separated by 117 nucleotidesPP_4660 and PP_4661 are separated by 183 nucleotides PP_4657: PP_4657 - zinc metalloprotease, at 5,282,474 to 5,283,424 _4657 PP_4658: PP_4658 - putative methyl-accepting chemotaxis protein, at 5,283,596 to 5,285,515 _4658 PP_4659: PP_4659 - gamma-glutamyltranspeptidase, at 5,285,638 to 5,287,305 _4659 PP_4660: PP_4660 - Transcriptional regulator, LysR family, at 5,287,423 to 5,288,319 _4660 PP_4661: PP_4661 - modulator of drug activity B, at 5,288,503 to 5,289,174 _4661
Group Condition PP_4657 PP_4658 PP_4659 PP_4660 PP_4661
nitrogen source Urea (N) -0.5 -0.1 +0.1 -2.0 -0.1
carbon source D-Glucose (C) -0.5 -0.2 -0.1 -1.1 -0.2
carbon source Crotonic (C) -0.9 -0.1 -0.0 -1.0 +0.0
phage P. put South MOI 10 -0.1 -0.2 -0.0 -1.4 -0.3
nitrogen source Urea (N); with MOPS -0.4 +0.0 -0.2 -1.3 -0.2
carbon source ferulate (C); with MOPS -0.1 +0.0 -0.1 -1.5 -0.2
stress MOPS minimal media_Glucose20mM with PHB-HV-HH 0.1 mg/mL -0.2 +0.0 -0.1 -1.6 +0.1
temperature Growth at 25C; with MOPS -0.0 -0.1 +0.0 -1.3 -0.3
stress Growth with Chloride 200 mM -0.2 -0.0 +0.1 -1.6 -0.0
nitrogen source Urea (N) -0.1 -0.5 +0.1 -1.4 +0.2
nitrogen source nitrate (N) -0.8 -0.5 -0.0 -0.7 +0.2
stress Growth with Chloride 500 mM; with MOPS +0.0 +0.1 -0.0 -1.6 -0.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 -0.2 -0.3 -1.2 +0.3
carbon source 2-Methylbutanoic (C) -0.2 +0.0 -0.0 -1.6 +0.3
nitrogen source NAG (N); with MOPS -0.5 -0.0 -0.1 -1.1 +0.3
nitrogen source Ammonium chloride (N); with MOPS -0.7 -0.2 -0.0 -0.4 +0.4
carbon source ferulate (C); with MOPS +0.1 +0.1 +0.1 -1.4 +0.1
carbon source ferulate (C); with MOPS -0.5 +0.3 +0.2 -0.7 +0.0
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 -0.1 +0.3 -1.0 +0.1
pH Growth at pH6 and (C) D-Glucose -0.7 -0.4 +0.1 +0.1 +0.5
nitrogen source D-2-Aminobutyric (N) -1.1 +0.0 -0.0 +0.4 +0.3
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay +0.6 +0.2 -0.2 -0.7 -0.1
carbon source carbon source Bisabolene 48.9 mM -0.6 -0.2 +0.1 +0.8 +0.2
carbon source carbon source 4-vinylphenol 0.01 mM +0.0 -0.3 -0.0 +1.2 -0.3
carbon source Octanoic (C) -0.3 -0.0 +0.2 +1.0 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.2 -0.2 -0.2 +1.0 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.5 +0.1 -0.3 +1.1 -0.6
pH pH 5 -0.0 -0.2 +0.2 +1.1 -0.2
pH pH 8 +0.1 -0.3 +0.1 +1.3 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.1 +0.4 -0.0 +0.7 +0.4
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