Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_1584 and PP_5476 are separated by 65 nucleotidesPP_5476 and PP_1585 are separated by 291 nucleotidesPP_1585 and PP_1586 are separated by 8 nucleotidesPP_1586 and PP_1587 are separated by 196 nucleotides PP_1584: PP_1584 - conserved protein of unknown function, at 1,776,398 to 1,776,898 _1584 PP_5476: PP_5476 - conserved protein of unknown function, at 1,776,964 to 1,777,266 _5476 PP_1585: PP_1585 - putative Antidote protein, at 1,777,558 to 1,777,857 _1585 PP_1586: PP_1586 - putative Killer protein, at 1,777,866 to 1,778,144 _1586 PP_1587: PP_1587 - NhaP-type Na+/H+ and K+/H+ antiporters, at 1,778,341 to 1,780,041 _1587
Group Condition PP_1584 PP_5476 PP_1585 PP_1586 PP_1587
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.1 +0.0 N.D. -0.2 -2.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.4 -0.7 N.D. -0.5 -0.3
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.3 -0.1 N.D. -0.2 -1.8
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.3 +0.0 N.D. -0.0 -1.9
carbon source carbon source 4-vinylphenol 0.01 mM -0.1 -1.1 N.D. +0.1 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.6 -0.5 N.D. -0.3 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 -0.3 N.D. -1.2 -0.1
carbon source D-Glucose (C) +0.2 -0.7 N.D. -0.6 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.4 -0.8 N.D. -0.3 +0.2
pH Growth at pH6 and (C) Trisodium citrate -1.1 +0.6 N.D. +0.0 +0.1
stress Growth with Chloride 200 mM; with MOPS +0.3 +0.1 N.D. -1.0 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.5 -0.7 N.D. -0.5 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.4 -0.7 N.D. +0.8 -0.0
nitrogen source nitrogen source nitrite 1mM -0.3 -0.5 N.D. +0.6 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.6 +0.2 N.D. -0.4 +0.6
stress Growth with Chloride 200 mM +0.3 +0.3 N.D. -0.9 +0.1
nitrogen source 2,6-diaminopimelic (N) +0.6 +0.3 N.D. -0.7 -0.0
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 +0.9 N.D. -0.6 +0.2
phage P. putida Sci small MOI 0.1 +0.9 +0.3 N.D. -0.7 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.1 -0.6 N.D. +0.4 -0.1
carbon source D-Fructose (C) +0.8 +0.4 N.D. -0.3 +0.0
carbon source Octanoic (C) +1.0 +0.5 N.D. -0.4 -0.2
carbon source D-Glucose (C) 2.5 mM +0.8 +0.8 N.D. -0.4 -0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.2 +1.2 N.D. +0.4 +0.0
carbon source Lauric (C) +0.9 +0.8 N.D. -0.1 -0.1
phage P. putida Missley MOI 0.1 -0.0 +0.1 N.D. +0.7 +0.8
carbon source 3-methyl-1-butanol 0.25 vol% (C) +0.3 +0.6 N.D. +0.4 +0.3
carbon source Valeric (C) +1.0 +1.0 N.D. -0.1 -0.2
carbon source Myristic (C) +1.2 +1.0 N.D. -0.1 +0.0
carbon source 1,3-Butandiol (C) +1.6 +1.1 N.D. +0.0 -0.0
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