Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS06160 and MMJJ_RS06165 are separated by 79 nucleotidesMMJJ_RS06165 and MMJJ_RS06170 are separated by 58 nucleotidesMMJJ_RS06170 and MMJJ_RS06175 are separated by 11 nucleotidesMMJJ_RS06175 and MMJJ_RS06180 are separated by 27 nucleotides MMJJ_RS06160: MMJJ_RS06160 - stage II sporulation protein M, at 1,142,444 to 1,143,034 _RS06160 MMJJ_RS06165: MMJJ_RS06165 - archaeosine synthase subunit alpha, at 1,143,114 to 1,144,730 _RS06165 MMJJ_RS06170: MMJJ_RS06170 - hypothetical protein, at 1,144,789 to 1,145,331 _RS06170 MMJJ_RS06175: MMJJ_RS06175 - DUF1743 domain-containing protein, at 1,145,343 to 1,146,248 _RS06175 MMJJ_RS06180: MMJJ_RS06180 - tryptophan--tRNA ligase, at 1,146,276 to 1,147,352 _RS06180
Group Condition MMJJ_RS06160 MMJJ_RS06165 MMJJ_RS06170 MMJJ_RS06175 MMJJ_RS06180
iron Iron limitation (0.2x), H2 media +0.1 -1.4 -2.8 -4.1 -4.0
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 +0.0 -3.4 -2.0 -3.2 -3.2
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.3 -2.0 -2.4 -3.8 -3.9
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.4 -2.0 -2.2 -3.7 -3.8
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.4 -1.9 -2.1 -3.8 -3.9
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.5 -1.9 -2.0 -3.8 -3.9
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.5 -1.9 -2.1 -3.7 -3.8
iron Iron excess (2.5x) , H2 media +0.6 -1.1 -2.8 -4.2 -3.2
hydrogen no stress control H2 no stress control +0.1 -1.2 -1.4 -3.3 -4.7
hydrogen no stress control H2 no stress control -0.0 -1.4 -1.9 -4.5 -2.7
stress Formate 50 mM hypophosphite +0.2 +0.0 -1.8 -4.6 -4.2
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.6 -1.6 -2.0 -3.5 -3.5
nitrogen source H2 N-free with 10mM D-glucosamine +0.1 -1.1 -2.7 -3.2 -3.1
nitrogen source H2 N-free with 10mM D-glucosamine +0.2 -1.3 -1.8 -3.3 -3.6
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.5 -1.8 -2.2 -3.0 -3.3
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE +0.3 -2.0 -1.3 -3.4 -3.2
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride +0.2 -1.1 -1.9 -2.9 -3.8
iron control, 1x iron with casaminos and vitamins, H2 media +0.2 -1.2 -2.4 -3.1 -3.0
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE +0.3 -1.0 -2.2 -2.8 -3.7
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.3 -1.7 -2.4 -2.8 -2.8
iron control, 1x iron with casaminos and vitamins, H2 media +0.2 -0.9 -2.0 -2.4 -4.1
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE +0.4 -0.8 -2.7 -2.4 -3.8
iron Iron limitation (0.2x), H2 media +0.2 -1.2 -2.2 -2.4 -3.6
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride +0.4 -1.3 -2.2 -3.3 -2.8
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.5 -1.3 -1.5 -3.4 -3.5
iron Iron excess (2.5x) , H2 media +0.7 -0.8 -2.5 -3.6 -2.6
stress McCas_formate_complete selenate 31.25 mM -0.1 +0.5 -1.1 -3.6 -4.6
nitrogen source H2 N-free with 2g/L casaminos +0.5 -1.3 -1.9 -4.3 -1.8
iron Iron excess (2.5x) , H2 media +0.7 -1.1 -2.6 -2.7 -2.9
nitrogen source Formate N-free with 2mM Adenosine +0.5 +2.1 -1.9 -2.7 -2.7
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