Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_10830 and PS417_10835 are separated by 136 nucleotidesPS417_10835 and PS417_10840 are separated by 19 nucleotidesPS417_10840 and PS417_10845 overlap by 12 nucleotidesPS417_10845 and PS417_10850 are separated by 219 nucleotides PS417_10830: PS417_10830 - LysR family transcriptional regulator, at 2,315,398 to 2,316,315 _10830 PS417_10835: PS417_10835 - major facilitator transporter, at 2,316,452 to 2,317,768 _10835 PS417_10840: PS417_10840 - amidase, at 2,317,788 to 2,318,561 _10840 PS417_10845: PS417_10845 - hypothetical protein, at 2,318,550 to 2,318,858 _10845 PS417_10850: PS417_10850 - histidine kinase, at 2,319,078 to 2,320,139 _10850
Group Condition PS417_10830 PS417_10835 PS417_10840 PS417_10845 PS417_10850
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.5 -1.4 N.D. N.D.
exudates RCH2_defined_glucose with Brachypodium distachyon 13-day exudates 1x N.D. -0.4 -0.8 N.D. N.D.
agar plate interaction control Taped volatile agar plate with no fungus N.D. -0.0 -1.1 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.2 -0.9 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +0.1 -1.1 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.0 -1.1 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.2 -0.8 N.D. N.D.
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x N.D. -0.3 -0.7 N.D. N.D.
exudates RCH2_defined_glucose with Brachypodium distachyon 27-day exudates 1x N.D. -0.1 -0.8 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.4 -0.6 N.D. N.D.
phage P. simiae OR Antrim MOI 10 N.D. -0.2 -0.7 N.D. N.D.
rhizosphere rhizosphere sample 2; outgrowth in LB N.D. -0.2 -0.7 N.D. N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. +0.0 -0.8 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +0.1 -0.8 N.D. N.D.
solid stress Fraxetin 1 mM; solid stress N.D. +0.1 -0.7 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.2 -0.6 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.4 -0.4 N.D. N.D.
seeds Growth on radish seeds for 72 hours N.D. -0.3 +0.6 N.D. N.D.
phage Wcs_1 N.D. -0.3 +0.9 N.D. N.D.
soil soil sample 7; outgrowth in LB N.D. -0.2 +0.8 N.D. N.D.
soil soil sample 4; outgrowth in LB N.D. -0.4 +1.0 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.2 +0.6 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.2 +0.6 N.D. N.D.
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X N.D. +0.1 +0.8 N.D. N.D.
carbon source NAG (C) N.D. +0.2 +0.7 N.D. N.D.
stress Nalidixic 0.025 mg/ml N.D. +0.2 +0.7 N.D. N.D.
carbon source octanoate (C) N.D. +0.0 +0.9 N.D. N.D.
nophagecontrol Only library N.D. +0.3 +0.6 N.D. N.D.
phage P. simiae Grant Run MOI 0.1 N.D. -0.4 +1.4 N.D. N.D.
seeds Growth on radish seeds for 72 hours N.D. +0.3 +1.2 N.D. N.D.
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