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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_10830 and PS417_10835 are separated by 136 nucleotides
PS417_10835 and PS417_10840 are separated by 19 nucleotides
PS417_10840 and PS417_10845 overlap by 12 nucleotides
PS417_10845 and PS417_10850 are separated by 219 nucleotides
PS417_10830: PS417_10830 - LysR family transcriptional regulator, at 2,315,398 to 2,316,315
_10830
PS417_10835: PS417_10835 - major facilitator transporter, at 2,316,452 to 2,317,768
_10835
PS417_10840: PS417_10840 - amidase, at 2,317,788 to 2,318,561
_10840
PS417_10845: PS417_10845 - hypothetical protein, at 2,318,550 to 2,318,858
_10845
PS417_10850: PS417_10850 - histidine kinase, at 2,319,078 to 2,320,139
_10850
Group
Condition
PS417
_10830
PS417
_10835
PS417
_10840
PS417
_10845
PS417
_10850
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.5
-1.4
N.D.
N.D.
exudates
RCH2_defined_glucose with Brachypodium distachyon 13-day exudates 1x
N.D.
-0.4
-0.8
N.D.
N.D.
agar plate interaction control
Taped volatile agar plate with no fungus
N.D.
-0.0
-1.1
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.2
-0.9
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
N.D.
+0.1
-1.1
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.0
-1.1
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.2
-0.8
N.D.
N.D.
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x
N.D.
-0.3
-0.7
N.D.
N.D.
exudates
RCH2_defined_glucose with Brachypodium distachyon 27-day exudates 1x
N.D.
-0.1
-0.8
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
-0.4
-0.6
N.D.
N.D.
phage
P. simiae OR Antrim MOI 10
N.D.
-0.2
-0.7
N.D.
N.D.
rhizosphere
rhizosphere sample 2; outgrowth in LB
N.D.
-0.2
-0.7
N.D.
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
+0.0
-0.8
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
N.D.
+0.1
-0.8
N.D.
N.D.
solid stress
Fraxetin 1 mM; solid stress
N.D.
+0.1
-0.7
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.2
-0.6
N.D.
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
+0.4
-0.4
N.D.
N.D.
seeds
Growth on radish seeds for 72 hours
N.D.
-0.3
+0.6
N.D.
N.D.
phage
Wcs_1
N.D.
-0.3
+0.9
N.D.
N.D.
soil
soil sample 7; outgrowth in LB
N.D.
-0.2
+0.8
N.D.
N.D.
soil
soil sample 4; outgrowth in LB
N.D.
-0.4
+1.0
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+0.2
+0.6
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+0.2
+0.6
N.D.
N.D.
supernatant control:fungal media
R2A with Vogels_fungal_media 0.8X
N.D.
+0.1
+0.8
N.D.
N.D.
carbon source
NAG (C)
N.D.
+0.2
+0.7
N.D.
N.D.
stress
Nalidixic 0.025 mg/ml
N.D.
+0.2
+0.7
N.D.
N.D.
carbon source
octanoate (C)
N.D.
+0.0
+0.9
N.D.
N.D.
nophagecontrol
Only library
N.D.
+0.3
+0.6
N.D.
N.D.
phage
P. simiae Grant Run MOI 0.1
N.D.
-0.4
+1.4
N.D.
N.D.
seeds
Growth on radish seeds for 72 hours
N.D.
+0.3
+1.2
N.D.
N.D.
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