Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS20170 and BPHYT_RS20175 are separated by 56 nucleotidesBPHYT_RS20175 and BPHYT_RS20180 overlap by 4 nucleotidesBPHYT_RS20180 and BPHYT_RS20185 are separated by 251 nucleotidesBPHYT_RS20185 and BPHYT_RS20190 are separated by 1859 nucleotides BPHYT_RS20170: BPHYT_RS20170 - sugar transporter, at 68,137 to 70,359 _RS20170 BPHYT_RS20175: BPHYT_RS20175 - protein-tyrosine-phosphatase, at 70,416 to 70,868 _RS20175 BPHYT_RS20180: BPHYT_RS20180 - sugar ABC transporter substrate-binding protein, at 70,865 to 71,917 _RS20180 BPHYT_RS20185: BPHYT_RS20185 - sugar transferase, at 72,169 to 73,530 _RS20185 BPHYT_RS20190: BPHYT_RS20190 - membrane protein, at 75,390 to 76,658 _RS20190
Group Condition BPHYT_RS20170 BPHYT_RS20175 BPHYT_RS20180 BPHYT_RS20185 BPHYT_RS20190
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.4 N.D. -1.4 -1.1 +0.0
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -0.6 N.D. -0.4 -2.1 +0.2
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.1 N.D. -0.4 -2.4 +0.3
phage 24hr PHYTO1 MOI 10 +0.0 N.D. -0.4 -1.8 -0.2
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.1 N.D. -0.6 -1.0 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.3 N.D. -1.8 -0.4 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 N.D. -1.2 -0.8 +0.2
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days -0.6 N.D. +0.2 -1.5 -0.2
phage 36hr PHYTO1 MOI 1 +0.2 N.D. -0.8 -0.6 -0.6
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.3 N.D. +0.4 -1.8 -0.6
phage 36hr PHYTO10 MOI 10 +0.9 N.D. +0.1 -2.4 -0.0
phage 24hr PHYTO3 MOI 10 +0.6 N.D. -0.4 -1.6 +0.0
phage 36hr PHYTO6 MOI 1 -1.0 N.D. +0.3 +0.3 -0.7
carbon source Phenylacetic 5 mM (C) -0.4 N.D. -1.0 +1.3 -0.5
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) +0.3 N.D. -1.1 +0.6 -0.2
nophagecontrol 36hr Phyto Lib only +0.5 N.D. -1.2 +0.3 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.0 N.D. +0.6 +0.7 -0.2
phage 36hr PHYTO4 MOI 1 +0.9 N.D. -0.5 +0.8 -0.1
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.6 N.D. +0.3 +1.2 +0.2
phage 24hr PHYTO6 MOI 10 +1.0 N.D. +0.3 -0.7 +0.4
phage P. phyto PHYTO2 MOI 1 -0.5 N.D. +0.6 +0.9 +0.3
phage 36hr PHYTO2 MOI 1 +0.9 N.D. +0.2 +1.0 -0.4
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.4 N.D. -0.3 +2.7 -0.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.4 N.D. -0.3 +2.6 -0.2
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X +0.7 N.D. -0.2 +1.0 +0.4
phage 24hr PHYTO4 MOI 0.1 +1.0 N.D. +0.7 +0.4 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 N.D. -0.0 +2.2 -0.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.2 N.D. -0.6 +3.1 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 N.D. +1.0 +1.7 -0.2
supernatant control Vogels_fungal_media; pH 5.5 +0.9 N.D. +0.9 +1.3 -0.1
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