Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_13560 and AO353_13565 are separated by 225 nucleotidesAO353_13565 and AO353_13570 are separated by 149 nucleotidesAO353_13570 and AO353_13575 are separated by 89 nucleotidesAO353_13575 and AO353_13580 are separated by 54 nucleotides AO353_13560: AO353_13560 - histidinol phosphatase, at 3,010,466 to 3,011,248 _13560 AO353_13565: AO353_13565 - peptidase, at 3,011,474 to 3,012,856 _13565 AO353_13570: AO353_13570 - TonB-dependent receptor, at 3,013,006 to 3,015,135 _13570 AO353_13575: AO353_13575 - hypothetical protein, at 3,015,225 to 3,015,644 _13575 AO353_13580: AO353_13580 - copper resistance protein CopZ, at 3,015,699 to 3,016,178 _13580
Group Condition AO353_13560 AO353_13565 AO353_13570 AO353_13575 AO353_13580
stress Nickel (II) chloride 0.625 mM N.D. +0.1 -0.4 -1.1 -1.8
carbon source L-Citrulline N.D. +0.2 +0.0 -2.0 -0.9
temperature LB 30C N.D. -0.2 -1.1 -0.5 -0.7
stress Doxycycline hyclate 0.0006 mg/ml N.D. +0.1 +0.0 -1.1 -1.4
temperature LB 30C N.D. +0.0 -0.7 -0.7 -0.8
motility outer cut, LB soft agar motility assay N.D. +0.0 -0.8 +0.6 -2.1
stress sodium fluoride 6.25 mM N.D. +0.3 -1.1 -0.2 -1.2
stress acetate 68 mM N.D. -0.4 -0.7 +0.1 -1.2
stress Benzalkonium Chloride 0.012 mg/ml N.D. +0.2 +0.2 -1.4 -1.1
temperature LB 20C N.D. +0.1 -0.6 -0.6 -0.9
stress Lomefloxacin 0.001 mM N.D. -0.1 -0.3 -1.2 -0.4
temperature LB 20C N.D. -0.2 -0.6 +0.0 -1.0
survival 4C survival; 360 hrs N.D. +0.3 -0.5 -0.8 -0.5
carbon source L-Citrulline N.D. +0.5 +0.3 +0.2 -1.6
carbon source L-Alanine (C) N.D. -0.0 +0.3 -1.3 +0.5
stress Cobalt chloride 0.32 mM N.D. +0.2 +0.9 +0.3 -1.8
anaerobic Anaerobic, acetate donor; nitrate and iron N.D. -0.7 +0.1 -0.5 +0.8
carbon source L-Ornithine N.D. +0.0 +0.4 +0.7 -1.2
carbon source L-Ornithine N.D. +0.6 +0.1 +0.5 -1.0
stress Fusidic 0.125 mg/ml N.D. +0.0 +0.5 -0.6 +0.8
carbon source L-Citrulline N.D. +0.7 +0.4 +0.3 -0.6
stress Cisplatin 0.006 mg/ml N.D. +0.3 +0.4 -0.5 +1.0
carbon source propionate (C) N.D. +0.2 +0.7 +0.3 +0.7
stress Paraquat dichloride 0.032 mg/mL N.D. +0.0 +1.1 +0.3 +0.5
carbon source propionate (C) N.D. +0.2 +0.8 +0.3 +0.5
carbon source acetate (C) N.D. +0.2 +1.2 -0.1 +0.6
pH Minimal media pH9 N.D. +0.2 +1.2 +0.8 -0.3
pH Minimal media pH9 N.D. -0.2 +1.5 +1.1 +0.6
carbon source acetate (C) N.D. +0.5 +1.4 +1.0 +0.8
stress Sisomicin 0.008 mg/ml N.D. +0.4 +2.8 +1.5 +2.2
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