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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
AO353_10320 and AO353_10325 overlap by 10 nucleotides
AO353_10325 and AO353_10330 are separated by 54 nucleotides
AO353_10330 and AO353_10335 overlap by 1 nucleotides
AO353_10335 and AO353_10340 overlap by 8 nucleotides
AO353_10320: AO353_10320 - hypothetical protein, at 2,263,688 to 2,263,918
_10320
AO353_10325: AO353_10325 - hypothetical protein, at 2,263,909 to 2,264,499
_10325
AO353_10330: AO353_10330 - linoleoyl-CoA desaturase, at 2,264,554 to 2,265,639
_10330
AO353_10335: AO353_10335 - linoleoyl-CoA desaturase, at 2,265,639 to 2,266,745
_10335
AO353_10340: AO353_10340 - fatty acid hydroxylase, at 2,266,738 to 2,267,877
_10340
Group
Condition
AO353
_10320
AO353
_10325
AO353
_10330
AO353
_10335
AO353
_10340
carbon source
L-Ornithine
-2.4
-0.2
-0.1
-0.2
-0.1
nitrogen source
NAG (N)
-2.3
-0.2
-0.0
-0.1
-0.0
stress
Furfuryl Alcohol 0.0625 vol%
-2.3
-0.0
-0.0
-0.2
-0.0
carbon source
L-Ornithine
-2.6
+0.0
+0.0
+0.0
+0.0
stress
copper (II) chloride 2.5 mM
-2.5
-0.2
+0.0
+0.2
+0.0
stress
methylglyoxal 0.009 vol%
-1.9
-0.3
+0.0
-0.3
+0.1
stress
Chlorite 0.16 mM
-2.2
-0.0
-0.0
-0.1
+0.2
carbon source
L-Alanine (C)
-2.3
-0.2
+0.3
-0.1
+0.0
stress
perchlorate 75 mM
-2.0
-0.1
+0.1
-0.0
-0.1
anaerobic
Anaerobic, acetate donor; nitrate and iron
-2.0
+0.2
-0.1
-0.1
-0.0
motility
outer cut, LB soft agar motility assay
-2.3
-0.2
+0.1
+0.2
+0.2
carbon source
a-Ketoglutaric (C)
-1.6
-0.2
-0.1
-0.0
-0.0
stress
Cholin acetate 19 mM
-1.4
-0.3
+0.1
-0.2
-0.2
carbon source
casaminos (C)
-1.8
+0.1
-0.0
+0.0
-0.1
carbon source
acetate (C)
-2.3
+0.3
-0.1
+0.1
+0.0
carbon source
succinate (C)
-1.5
-0.2
-0.1
+0.1
-0.0
carbon source
L-Malic (C)
-1.9
+0.2
+0.0
-0.0
+0.1
stress
acetate 34 mM
-1.6
+0.1
-0.0
+0.1
-0.1
stress
Spectinomycin 0.15 mg/ml
-1.3
-0.0
-0.1
+0.1
-0.2
carbon source
propionate (C)
-1.8
+0.0
+0.2
+0.0
+0.0
denitrifying
Nitrate reduction, replicate C
-1.4
+0.2
+0.0
-0.3
-0.1
carbon source
Tween 20 (C)
-1.4
-0.0
-0.1
+0.1
+0.0
stress
Cholin acetate 10 mM
-1.5
-0.0
+0.2
+0.0
-0.0
stress
Rifampicin 0.005 mg/ml
-1.2
-0.1
-0.2
+0.2
-0.1
carbon source
L-Ornithine
-1.3
+0.1
-0.1
+0.1
+0.1
denitrifying
Nitrate reduction, replicate B
-1.2
+0.3
+0.1
-0.1
+0.0
carbon source
D-Serine (C)
+1.3
-0.1
-0.1
-0.1
-0.1
pH
Minimal media pH9
+0.8
+0.4
+0.2
-0.1
-0.1
motility
outer cut, LB soft agar motility assay
+0.4
+0.2
-0.2
+0.4
+0.5
nitrogen source
L-Glutamine (N)
+1.4
-0.0
-0.1
-0.1
+0.1
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