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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
tdcE and tdcD are separated by 33 nucleotides
tdcD and tdcC are separated by 25 nucleotides
tdcC and tdcB are separated by 21 nucleotides
tdcB and tdcA are separated by 98 nucleotides
b3114: tdcE - pyruvate formate-lyase 4/2-ketobutyrate formate-lyase (NCBI), at 3,258,146 to 3,260,440
tdcE
b3115: tdcD - propionate kinase/acetate kinase C, anaerobic (RefSeq), at 3,260,474 to 3,261,682
tdcD
b3116: tdcC - L-threonine/L-serine transporter (NCBI), at 3,261,708 to 3,263,039
tdcC
b3117: tdcB - threonine dehydratase (NCBI), at 3,263,061 to 3,264,050
tdcB
b3118: tdcA - DNA-binding transcriptional activator (NCBI), at 3,264,149 to 3,265,087
tdcA
Group
Condition
b3114
b3115
b3116
b3117
b3118
tdcE
tdcD
tdcC
tdcB
tdcA
motility
outer cut, LB soft agar motility assay
-0.2
-0.9
-0.7
-0.4
-0.5
stress
LB + carbenicillin regrowth after 48hr
-0.3
-0.8
-0.6
-0.6
-0.3
stress
LB + carbenicillin regrowth after 48hr
-0.2
-0.6
-0.7
-0.6
-0.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.3
-0.1
-0.2
-0.9
-0.9
motility
outer cut, LB soft agar motility assay
-0.3
-0.5
-0.3
-0.5
-0.7
stress
Cephalothin 0.01 mg/ml
-0.1
+0.0
-1.9
-0.1
-0.2
mixed community
Mixed culture; Bdellovibrio bacteriovorus HD100 1:1 starting OD to Ecoli; 0.1X M9; outgrowth in LBwithKan
-0.2
-0.4
-0.3
-0.4
-0.4
stress
LB + ciprofloxacin regrowth after 48hrs
-0.1
-0.6
-0.5
-0.3
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.3
-0.2
+0.0
-0.5
-0.7
mzaabcde
mzaABCDE
-0.3
+0.0
-0.1
-0.7
-0.5
stress
LB + carbenicillin Time9hr
-0.2
-0.6
-0.3
-0.3
-0.1
plasmid transformation
vcDART vector transformation; agar assay
-0.2
-0.1
-0.2
-0.7
-0.4
motility
outer cut, LB soft agar motility assay
-0.2
-0.1
-0.5
-0.3
-0.5
gfp_control
GFP_control
-0.2
-0.0
-0.1
-0.8
-0.3
lb
LB regrowth after 48hrs
-0.1
-0.6
-0.4
-0.2
-0.1
plasmid transformation
vcDART vector transformation; agar assay
-0.1
-0.1
-0.1
-0.7
-0.4
stress
LB Anaerobic with Zinc Pyrithione 0.0000025 mM
-0.1
-0.2
-0.3
-0.1
-0.6
stress
LB + carbenicillin Time8hr
-0.2
-0.5
-0.2
-0.4
-0.2
stress
LB + carbenicillin Time9hr
-0.1
-0.5
-0.2
-0.3
-0.2
retron_ec_48_control
retron_Ec_48_control
-0.1
+0.1
-0.1
-0.8
-0.3
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-0.2
-0.4
-0.1
+0.1
-0.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.2
-0.0
-0.1
-0.6
-0.6
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.2
-0.0
+0.3
-0.5
-0.7
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.2
-0.1
+0.3
-0.4
-0.5
stress
Benzalkonium Chloride 0.004 mg/ml
+0.1
-0.0
-1.7
+0.7
+0.5
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.3
-0.2
-0.0
+0.4
+0.5
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
+0.4
+0.8
+0.2
-0.2
+0.1
phage
T7_phage 0.0015625 MOI TC1
+0.2
+0.5
+0.3
+0.2
+0.7
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
+0.4
+0.9
+0.4
-0.0
+0.4
stress
Chlorite 1.25 mM
+1.0
+0.4
+0.4
+0.5
+0.3
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