Fitness for 5 genes in Escherichia coli BW25113

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500 nttdcE and tdcD are separated by 33 nucleotidestdcD and tdcC are separated by 25 nucleotidestdcC and tdcB are separated by 21 nucleotidestdcB and tdcA are separated by 98 nucleotides b3114: tdcE - pyruvate formate-lyase 4/2-ketobutyrate formate-lyase (NCBI), at 3,258,146 to 3,260,440 tdcE b3115: tdcD - propionate kinase/acetate kinase C, anaerobic (RefSeq), at 3,260,474 to 3,261,682 tdcD b3116: tdcC - L-threonine/L-serine transporter (NCBI), at 3,261,708 to 3,263,039 tdcC b3117: tdcB - threonine dehydratase (NCBI), at 3,263,061 to 3,264,050 tdcB b3118: tdcA - DNA-binding transcriptional activator (NCBI), at 3,264,149 to 3,265,087 tdcA
Group Condition b3114 b3115 b3116 b3117 b3118
tdcE tdcD tdcC tdcB tdcA
motility outer cut, LB soft agar motility assay -0.2 -0.9 -0.7 -0.4 -0.5
stress LB + carbenicillin regrowth after 48hr -0.3 -0.8 -0.6 -0.6 -0.3
stress LB + carbenicillin regrowth after 48hr -0.2 -0.6 -0.7 -0.6 -0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.3 -0.1 -0.2 -0.9 -0.9
motility outer cut, LB soft agar motility assay -0.3 -0.5 -0.3 -0.5 -0.7
stress Cephalothin 0.01 mg/ml -0.1 +0.0 -1.9 -0.1 -0.2
mixed community Mixed culture; Bdellovibrio bacteriovorus HD100 1:1 starting OD to Ecoli; 0.1X M9; outgrowth in LBwithKan -0.2 -0.4 -0.3 -0.4 -0.4
stress LB + ciprofloxacin regrowth after 48hrs -0.1 -0.6 -0.5 -0.3 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.3 -0.2 +0.0 -0.5 -0.7
mzaabcde mzaABCDE -0.3 +0.0 -0.1 -0.7 -0.5
stress LB + carbenicillin Time9hr -0.2 -0.6 -0.3 -0.3 -0.1
plasmid transformation vcDART vector transformation; agar assay -0.2 -0.1 -0.2 -0.7 -0.4
motility outer cut, LB soft agar motility assay -0.2 -0.1 -0.5 -0.3 -0.5
gfp_control GFP_control -0.2 -0.0 -0.1 -0.8 -0.3
lb LB regrowth after 48hrs -0.1 -0.6 -0.4 -0.2 -0.1
plasmid transformation vcDART vector transformation; agar assay -0.1 -0.1 -0.1 -0.7 -0.4
stress LB Anaerobic with Zinc Pyrithione 0.0000025 mM -0.1 -0.2 -0.3 -0.1 -0.6
stress LB + carbenicillin Time8hr -0.2 -0.5 -0.2 -0.4 -0.2
stress LB + carbenicillin Time9hr -0.1 -0.5 -0.2 -0.3 -0.2
retron_ec_48_control retron_Ec_48_control -0.1 +0.1 -0.1 -0.8 -0.3
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.2 -0.4 -0.1 +0.1 -0.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.2 -0.0 -0.1 -0.6 -0.6
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.2 -0.0 +0.3 -0.5 -0.7
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.2 -0.1 +0.3 -0.4 -0.5
stress Benzalkonium Chloride 0.004 mg/ml +0.1 -0.0 -1.7 +0.7 +0.5
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.3 -0.2 -0.0 +0.4 +0.5
stress LB Anaerobic with Amoxicillin 0.0000078125 mM +0.4 +0.8 +0.2 -0.2 +0.1
phage T7_phage 0.0015625 MOI TC1 +0.2 +0.5 +0.3 +0.2 +0.7
stress LB Anaerobic with Amoxicillin 0.0000078125 mM +0.4 +0.9 +0.4 -0.0 +0.4
stress Chlorite 1.25 mM +1.0 +0.4 +0.4 +0.5 +0.3
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