Fitness for 5 genes in Escherichia coli BW25113

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500 ntyoeF and yeeX are separated by 100 nucleotidesyeeX and yeeA are separated by 171 nucleotidesyeeA and sbmC are separated by 197 nucleotidessbmC and dacD are separated by 118 nucleotides b4538: yoeF - hypothetical protein (RefSeq), at 2,076,770 to 2,076,955 yoeF b2007: yeeX - conserved protein (RefSeq), at 2,077,056 to 2,077,385 yeeX b2008: yeeA - conserved inner membrane protein (NCBI), at 2,077,557 to 2,078,615 yeeA b2009: sbmC - DNA gyrase inhibitor (NCBI), at 2,078,813 to 2,079,286 sbmC b2010: dacD - penicillin binding protein 6b (VIMSS), at 2,079,405 to 2,080,571 dacD
Group Condition b4538 b2007 b2008 b2009 b2010
yoeF yeeX yeeA sbmC dacD
carbon source carbon source D-Mannose 5mM -3.7 -1.1 -0.2 +0.3 +0.2
phage N4_phage 0.009375 MOI TC2 -2.8 -0.9 +0.1 +0.0 -0.4
carbon source carbon source D-Glucose 0.25mM -2.1 -1.3 +0.1 +0.0 -0.5
gfp_control GFP_control -0.3 -1.1 -0.5 -1.1 -0.5
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -1.6 -0.3 -0.3 -1.0 -0.1
control PBS_cage_1_mice _Feces_16 hr -2.6 -0.0 +0.4 -0.5 -0.3
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -1.8 -0.4 -0.8 -0.6 +0.6
phage RhdML5 -3.1 -0.1 -0.3 +0.8 +0.0
stress Nickel (II) chloride 1 mM +0.5 -3.4 +0.2 -0.0 +0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay -3.7 +0.8 +0.1 +0.7 -0.2
stress Spectinomycin 0.00625 mg/ml +0.6 -2.9 -0.2 +0.1 +0.1
phage HSDP4 -4.5 +1.1 +0.3 +0.9 +0.1
mzaabcde mzaABCDE +0.8 -1.7 -0.3 -0.6 -0.2
stress Nickel (II) chloride 1.5 mM +1.3 -3.0 -0.0 +0.3 +0.0
carbon source carbon source D-Mannose 20mM -1.6 +0.8 +0.2 -0.6 +0.2
phage Ste41 -2.9 +0.8 +0.0 +0.9 +0.2
phage N4_phage 0.0009375 MOI TC2 +1.0 -1.0 -0.2 +0.3 -0.8
phage Δdbn_10 +0.9 -1.2 +0.7 -0.7 -0.1
motility inner cut, LB soft agar motility assay +1.4 -1.4 -0.1 +0.3 -0.2
stress Aluminum chloride 5 mM -1.4 +1.5 -0.1 -0.2 +0.3
micoeukaryotes Tetrahymena sp., Mixed culture-1 +1.5 -0.4 -0.9 +0.4 -0.4
stress LB Aerobic with Irgasan 0.0000003125 mM -0.8 +1.8 -0.0 +0.3 -0.6
predation 6 hr rep1_HD100_10fold_dilution_grewON +1.2 +0.7 +0.7 -1.1 -0.2
carbon source carbon source D-Mannose 1mM +1.5 +0.4 +0.5 +0.7 -0.7
control PBS_cage_2_mouse_3_Cecum_40 hr +1.0 +1.9 -0.0 -0.2 -0.3
stress Benzalkonium Chloride 0.004 mg/ml +1.4 +1.5 -0.4 -0.3 +0.2
motility outer cut, LB soft agar motility assay +1.1 +0.2 +0.4 +1.5 -0.1
phage T7_phage 0.00015625 MOI TC4 +1.4 -0.4 +0.8 +1.1 +0.4
phage T7_phage 0.00015625 MOI TC3 +1.7 +0.1 +0.4 +0.8 +0.5
control PBS_cage_2_mouse_2_Cecum_40 hr -0.3 +1.7 +1.0 +1.2 -0.0
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