Fitness for 5 genes in Escherichia coli BW25113

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500 ntyeaI and yeaJ are separated by 180 nucleotidesyeaJ and yeaK are separated by 42 nucleotidesyeaK and b1788 are separated by 0 nucleotidesb1788 and yeaL are separated by 169 nucleotides b1785: yeaI - predicted diguanylate cyclase (NCBI), at 1,868,409 to 1,869,884 yeaI b1786: yeaJ - orf, hypothetical protein (VIMSS), at 1,870,065 to 1,871,555 yeaJ b1787: yeaK - hypothetical protein (NCBI), at 1,871,598 to 1,872,101 yeaK b1788: b1788 - orf, hypothetical protein (VIMSS), at 1,872,102 to 1,872,206 b1788 b1789: yeaL - conserved inner membrane protein (NCBI), at 1,872,376 to 1,872,822 yeaL
Group Condition b1785 b1786 b1787 b1788 b1789
yeaI yeaJ yeaK b1788 yeaL
carbon source D-Galacturonic Acid (C) M9 -0.1 +0.1 -0.5 N.D. -2.8
carbon source D-Galacturonic Acid (C) MOPS -0.0 -0.0 -0.1 N.D. -3.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.7 +0.0 -0.5 N.D. -1.4
phage T7_phage 0.0015625 MOI TC2 +0.4 +0.2 -0.2 N.D. -1.6
control PBS_cage_2_mouse_1_Cecum_40 hr -1.5 -0.1 -0.6 N.D. +1.0
carbon source carbon source D-Glucose 0.25mM -1.7 -0.1 -0.4 N.D. +1.1
phage Ste41 +0.9 -0.2 -0.8 N.D. +0.5
phage Ste41 +1.3 -0.5 -1.2 N.D. +0.9
phage R2_N4_T2_phage10 -0.8 +0.5 -0.1 N.D. +1.3
motility inner cut, LB soft agar motility assay -0.6 +0.1 +0.2 N.D. +1.4
phage Goslar -0.9 +2.2 -0.1 N.D. +0.1
phage Goslar -0.4 +2.0 +0.0 N.D. -0.2
phage R1_N4_T2_phage8 -0.4 +1.8 +0.6 N.D. -0.2
control PBS_cage_1_mouse_1_Cecum_40 hr +1.9 -0.1 +0.6 N.D. -0.1
phage Papa dilution 10-1 cacl2 maltose +1.2 +1.1 +0.0 N.D. -0.0
phage TP15 -0.0 +2.3 +0.1 N.D. -0.0
stress Trimethoprim 0.005 mM +2.2 +0.2 +0.7 N.D. +0.3
phage N4_phage 0.09375 MOI TC1 +1.1 +4.0 +0.1 N.D. -1.5
phage N4_phage 0.00009375 MOI TC1 -0.3 +3.6 +0.3 N.D. +0.1
phage No phage control ML9a -0.6 +4.0 +0.3 N.D. +0.3
phage LB_plus_SM_buffer -0.4 +4.7 -0.0 N.D. -0.0
phage N4_phage 0.00009375 MOI TC3 +0.3 +4.9 +0.0 N.D. -0.7
phage N4_phage 0.009375 MOI TC1 +0.6 +5.5 -0.7 N.D. -0.6
phage N4_phage 0.00009375 MOI TC5 +0.5 +6.0 -0.2 N.D. -0.6
phage N4_phage 0.0009375 MOI TC3 +1.1 +6.8 -0.0 N.D. -1.1
phage N4_phage 0.0009375 MOI TC2 +0.7 +6.5 -0.1 N.D. -0.2
phage N4_phage 0.00009375 MOI TC4 +0.8 +7.3 +0.3 N.D. -0.7
ms2_1000_b3 MS2_1000_B3 +0.3 +7.4 +0.3 N.D. -0.1
ms2_1000_a3 MS2_1000_A3 +0.6 +8.1 +0.1 N.D. +0.0
phage N4_phage 0.009375 MOI TC2 +2.2 +7.5 -0.3 N.D. -0.0
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