Experiment set6IT021 for Methanococcus maripaludis S2

200 most detrimental genes:

gene	name	fitness	t score	description
MMP_RS01370		+2.5	15.8	acetate--CoA ligase family protein
MMP_RS06575		+1.6	10.0	fluoride efflux transporter CrcB
MMP_RS01775		+1.3	3.5	TIGR00297 family protein
MMP_RS00750		+1.3	3.2	fumarate hydratase
MMP_RS06770		+1.2	6.8	DHH family phosphoesterase
MMP_RS07415		+1.2	7.3	molybdopterin-guanine dinucleotide biosynthesis protein MobB
MMP_RS03320		+1.2	4.5	metal-dependent transcriptional regulator
MMP_RS03420		+1.1	6.9	acetolactate synthase small subunit
MMP_RS07115		+1.1	7.8	MBL fold metallo-hydrolase
MMP_RS03390		+1.1	5.0	malate dehydrogenase
MMP_RS03215		+1.0	9.0	MBL fold metallo-hydrolase
MMP_RS05535		+1.0	3.7	hypothetical protein
MMP_RS08360		+1.0	5.1	hypothetical protein
MMP_RS08365		+1.0	5.0	energy conserving hydrogenase EhbF
MMP_RS08355		+1.0	4.9	Na(+)/H(+) antiporter subunit B
MMP_RS06570		+1.0	2.8	DUF190 domain-containing protein
MMP_RS06600		+1.0	2.3	class III signal peptide-containing protein
MMP_RS08345		+0.9	6.9	4Fe-4S binding protein
MMP_RS08370		+0.9	1.0	cation:proton antiporter subunit C
MMP_RS05950		+0.9	4.9	nickel-dependent hydrogenase large subunit
MMP_RS08335		+0.9	6.0	NADH-quinone oxidoreductase subunit B family protein
MMP_RS08350		+0.8	1.1	hypothetical protein
MMP_RS06395		+0.8	3.4	ThiF family adenylyltransferase
MMP_RS07850		+0.8	3.7	prephenate dehydratase
MMP_RS07820		+0.8	3.7	UPF0104 family protein
MMP_RS06325		+0.7	1.8	hypothetical protein
MMP_RS08340		+0.7	3.5	4Fe-4S binding protein
MMP_RS00325		+0.7	2.0	RNA-binding protein
MMP_RS07955		+0.7	2.8	FumA C-terminus/TtdB family hydratase beta subunit
MMP_RS07120		+0.7	5.6	coenzyme F420 hydrogenase subunit alpha
MMP_RS00725		+0.7	3.8	5 10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdC
MMP_RS07125		+0.7	3.7	coenzyme F420-reducing hydrogenase FrhD protein
MMP_RS08275		+0.7	4.5	hypothetical protein
MMP_RS00735		+0.7	2.4	5 10-methenyltetrahydromethanopterin hydrogenase
MMP_RS03450		+0.7	1.6	glycine synthesis protein GlyXS (from data)
MMP_RS07725		+0.6	2.8	4Fe-4S dicluster domain-containing protein
MMP_RS06335		+0.6	2.8	HAD family hydrolase
MMP_RS07135		+0.6	4.0	coenzyme F420 hydrogenase subunit beta
MMP_RS07615		+0.6	3.7	energy-coupling factor ABC transporter permease
MMP_RS08760		+0.6	1.3	hypothetical protein
MMP_RS08400		+0.6	2.7	MJ0307 family thioredoxin
MMP_RS06555		+0.6	2.7	cupin domain-containing protein
MMP_RS07355		+0.6	1.1	transcription factor S
MMP_RS04265		+0.6	2.4	coenzyme F420-reducing hydrogenase FrhD protein
MMP_RS00450		+0.6	2.0	4Fe-4S binding protein
MMP_RS07410		+0.6	4.1	4-demethylwyosine synthase TYW1
MMP_RS01540		+0.6	1.8	DUF504 domain-containing protein
MMP_RS08705		+0.6	3.3	sulfopyruvate decarboxylase subunit beta
MMP_RS03385		+0.6	1.1	cation diffusion facilitator family transporter
MMP_RS02805		+0.6	1.1	HEPN domain-containing protein
MMP_RS05850		+0.6	1.7	Ldh family oxidoreductase
MMP_RS01415		+0.6	2.3	DUF2067 domain-containing protein
MMP_RS00530		+0.5	3.8	tRNA pseudouridine(54/55) synthase Pus10
MMP_RS01525		+0.5	1.6	nucleoside-diphosphate kinase
MMP_RS07695		+0.5	3.4	DUF3236 domain-containing protein
MMP_RS00665		+0.5	2.6	hypothetical protein
MMP_RS00730		+0.5	2.5	5 10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB
MMP_RS07700		+0.5	3.2	DUF1188 domain-containing protein
MMP_RS07620		+0.5	2.4	energy-coupling factor ABC transporter substrate-binding protein
MMP_RS08470		+0.5	3.5	ATP-binding cassette domain-containing protein
MMP_RS01115		+0.5	3.1	molybdopterin molybdotransferase MoeA
MMP_RS01475		+0.5	3.2	phosphosulfolactate synthase
MMP_RS07130		+0.5	3.1	coenzyme F420 hydrogenase subunit gamma
MMP_RS07435		+0.5	1.2	GMP synthase subunit A
MMP_RS01620		+0.5	2.2	hypothetical protein
MMP_RS00055		+0.5	3.6	DUF2254 domain-containing protein
MMP_RS04850		+0.5	3.9	UPF0254 family protein
MMP_RS08995		+0.5	0.9	hypothetical protein
MMP_RS06435		+0.5	3.5	CBS domain-containing protein
MMP_RS06535		+0.5	2.2	2-oxoacid:acceptor oxidoreductase family protein
MMP_RS03890		+0.5	2.5	DUF2540 domain-containing protein
MMP_RS08595		+0.4	0.5	flagellar protein C
MMP_RS00880		+0.4	2.0	ribosome assembly RNA-binding protein YhbY
MMP_RS01435		+0.4	3.0	DMT family transporter
MMP_RS00545		+0.4	2.0	XRE family transcriptional regulator
MMP_RS00560		+0.4	3.3	cation:proton antiporter
MMP_RS07890		+0.4	1.3	OB-fold nucleic acid binding domain-containing protein
MMP_RS06290		+0.4	2.8	DNA repair and recombination protein RadA
MMP_RS06530		+0.4	2.4	bifunctional hexulose-6-phosphate synthase/ribonuclease regulator
MMP_RS07335		+0.4	2.2	tRNA (cytidine(56)-2'-O)-methyltransferase
MMP_RS08775		+0.4	2.3	methanogenesis marker 9 domain-containing protein
MMP_RS01100		+0.4	1.0	ABC transporter ATP-binding protein
MMP_RS02310		+0.4	1.1	RimK-like ATPgrasp N-terminal domain-containing protein
MMP_RS00655		+0.4	1.9	hypothetical protein
MMP_RS05970		+0.4	1.4	class II SORL domain-containing protein
MMP_RS02875		+0.4	2.7	chorismate pyruvate-lyase family protein
MMP_RS02960		+0.4	2.9	class I SAM-dependent methyltransferase family protein
MMP_RS00315		+0.4	1.7	Nif3-like dinuclear metal center hexameric protein
MMP_RS02870		+0.4	2.6	hypothetical protein
MMP_RS05525		+0.4	2.4	calcium/sodium antiporter
MMP_RS05450		+0.4	1.7	CoB--CoM heterodisulfide reductase subunit C
MMP_RS01650		+0.4	0.6	sulfurtransferase TusA family protein
MMP_RS08280		+0.4	1.6	proteasome assembly chaperone family protein
MMP_RS03840		+0.4	2.2	hypothetical protein
MMP_RS00900		+0.4	1.2	50S ribosomal protein L39e
MMP_RS00660		+0.4	2.9	alkaline phosphatase family protein
MMP_RS07540		+0.4	2.1	CBS domain-containing protein
MMP_RS00185		+0.4	3.2	potassium channel protein
MMP_RS08415		+0.4	1.3	radical SAM protein
MMP_RS00395		+0.4	1.0	MGMT family protein
MMP_RS06540		+0.4	1.5	3-methyl-2-oxobutanoate dehydrogenase subunit VorB
MMP_RS04740		+0.4	2.2	DUF473 domain-containing protein
MMP_RS05070		+0.4	1.9	4Fe-4S dicluster domain-containing protein
MMP_RS00285		+0.4	2.2	TIGR04013 family B12-binding domain/radical SAM domain-containing protein
MMP_RS01950		+0.4	2.9	hypothetical protein
MMP_RS07900		+0.4	0.8	YeeE/YedE family protein
MMP_RS06155		+0.4	1.6	DUF4186 domain-containing protein
MMP_RS06550		+0.4	2.3	AMP-binding protein
MMP_RS05675		+0.4	1.5	phosphate ABC transporter ATP-binding protein PstB
MMP_RS04520		+0.4	2.0	nucleoside deaminase
MMP_RS03245		+0.4	2.3	DNA repair and recombination protein RadB
MMP_RS00955		+0.3	2.7	coenzyme gamma-F420-2:alpha-L-glutamate ligase
MMP_RS07960		+0.3	2.2	sugar phosphate isomerase/epimerase
MMP_RS08835		+0.3	2.7	H(2)-dependent methylenetetrahydromethanopterin dehydrogenase-related protein
MMP_RS00100		+0.3	2.6	tRNA pseudouridine(13) synthase TruD
MMP_RS03115		+0.3	2.7	hypothetical protein
MMP_RS01430		+0.3	2.1	mechanosensitive ion channel family protein
MMP_RS00940		+0.3	2.1	daunorubicin resistance protein DrrA family ABC transporter ATP-binding protein
MMP_RS05170		+0.3	2.0	lysine exporter LysO family protein
MMP_RS02895		+0.3	1.8	DHH family phosphoesterase
MMP_RS04090		+0.3	1.0	hypothetical protein
MMP_RS04195		+0.3	1.7	flavodoxin
MMP_RS03675		+0.3	2.4	RimK family alpha-L-glutamate ligase
MMP_RS00635		+0.3	2.1	energy-coupling factor transporter transmembrane protein EcfT
MMP_RS06365		+0.3	1.2	hypothetical protein
MMP_RS00280		+0.3	2.2	DUF2118 domain-containing protein
MMP_RS00140		+0.3	1.6	DUF192 domain-containing protein
MMP_RS00565		+0.3	1.7	CBS domain-containing protein
MMP_RS01960		+0.3	0.3	hypothetical protein
MMP_RS08265		+0.3	1.3	rubrerythrin
MMP_RS02495		+0.3	0.9	hypothetical protein
MMP_RS01930		+0.3	2.5	hypothetical protein
MMP_RS01580		+0.3	2.3	PQQ-binding-like beta-propeller repeat protein
MMP_RS02265		+0.3	1.3	nitroreductase family protein
MMP_RS08315		+0.3	1.3	S-methyl-5-thioribose-1-phosphate isomerase
MMP_RS00150		+0.3	1.7	AAA family ATPase
MMP_RS04720		+0.3	0.8	DedA family protein
MMP_RS08455		+0.3	0.8	multiprotein bridging factor aMBF1
MMP_RS05295		+0.3	1.7	minichromosome maintenance protein MCM
MMP_RS05945		+0.3	1.6	CBS domain-containing protein
MMP_RS02745		+0.3	0.9	hypothetical protein
MMP_RS00135		+0.3	2.2	DUF2121 domain-containing protein
MMP_RS05825		+0.3	1.4	peptidyl-prolyl cis-trans isomerase
MMP_RS03765		+0.3	1.6	CopG family ribbon-helix-helix protein
MMP_RS04595		+0.3	1.6	hypothetical protein
MMP_RS01940		+0.3	1.1	MoxR family ATPase
MMP_RS00105		+0.3	1.9	hypothetical protein
MMP_RS06300		+0.3	1.8	ABC transporter substrate-binding protein
MMP_RS04015		+0.3	0.7	hypothetical protein
MMP_RS01110		+0.3	0.9	FmdE family protein
MMP_RS08700		+0.3	1.8	hypothetical protein
MMP_RS02475		+0.3	0.8	hypothetical protein
MMP_RS00085		+0.3	1.9	DNA cytosine methyltransferase
MMP_RS02290		+0.3	1.9	MBL fold metallo-hydrolase
MMP_RS01945		+0.3	1.6	DUF4350 domain-containing protein
MMP_RS05975		+0.3	0.6	hypothetical protein
MMP_RS06545		+0.3	1.1	ferredoxin family protein
MMP_RS07815		+0.3	0.5	DUF2120 domain-containing protein
MMP_RS02085		+0.2	1.7	inosine/xanthosine triphosphatase
MMP_RS09030		+0.2	1.4	hypothetical protein
MMP_RS08130		+0.2	1.8	IGHMBP2 family helicase
MMP_RS02720		+0.2	1.2	molybdate ABC transporter substrate-binding protein
MMP_RS05990		+0.2	1.0	DsrE family protein
MMP_RS06965		+0.2	1.0	TIGR04014 family B12-binding domain/radical SAM domain-containing protein
MMP_RS04480		+0.2	2.1	lysine 2 3-aminomutase
MMP_RS03395		+0.2	1.1	hypothetical protein
MMP_RS03150		+0.2	2.0	phosphoglycerate mutase
MMP_RS01055		+0.2	0.7	hypothetical protein
MMP_RS04965		+0.2	1.5	thioredoxin family protein
MMP_RS05265		+0.2	0.4	2-isopropylmalate synthase
MMP_RS01690		+0.2	1.4	indolepyruvate ferredoxin oxidoreductase subunit alpha
MMP_RS00630		+0.2	1.3	ECF transporter S component
MMP_RS08555		+0.2	1.0	hypothetical protein
MMP_RS05980		+0.2	1.5	ferritin
MMP_RS00375		+0.2	1.5	ammonium transporter
MMP_RS01260		+0.2	1.0	HDIG domain-containing protein
MMP_RS07770		+0.2	1.3	alanine racemase
MMP_RS08655		+0.2	1.8	phosphoadenosine phosphosulfate reductase family protein
MMP_RS01455		+0.2	1.4	nickel pincer cofactor biosynthesis protein LarC
MMP_RS07595		+0.2	1.6	hypothetical protein
MMP_RS00800		+0.2	1.6	Coenzyme F420 hydrogenase/dehydrogenase beta subunit C-terminal domain
MMP_RS07555		+0.2	0.3	monovalent cation/H+ antiporter subunit E
MMP_RS04425		+0.2	1.9	APC family permease
MMP_RS08500		+0.2	0.9	pyridoxal 5'-phosphate synthase glutaminase subunit PdxT
MMP_RS00945		+0.2	0.5	PadR family transcriptional regulator
MMP_RS06485		+0.2	0.3	zinc ribbon domain-containing protein
MMP_RS01185		+0.2	1.3	hypothetical protein
MMP_RS00385		+0.2	1.5	P-II family nitrogen regulator
MMP_RS02785		+0.2	1.2	MarR family transcriptional regulator
MMP_RS06440		+0.2	1.0	CBS domain-containing protein
MMP_RS01130		+0.2	1.0	flavoprotein
MMP_RS08160		+0.2	1.2	EamA family transporter
MMP_RS08260		+0.2	1.4	DUF99 family protein
MMP_RS00645		+0.2	1.5	iron ABC transporter permease
MMP_RS01515		+0.2	1.5	PINc/VapC family ATPase
MMP_RS02380		+0.2	1.4	ATP-binding protein
MMP_RS05600		+0.2	1.8	AglZ/HisF2 family acetamidino modification protein
MMP_RS06765		+0.2	1.1	flap endonuclease-1
MMP_RS05505		+0.2	1.4	class IV adenylate cyclase
MMP_RS06745		+0.2	0.9	DUF2119 domain-containing protein

Specific Phenotypes

None in this experiment

For Methanococcus maripaludis S2 in nutrient t1 experiments