Experiment set53IT058 for Escherichia coli BW25113

Compare to:

M9 no stress control; Anaerobic 48 hr

200 most detrimental genes:

  gene name fitness t score description  
b3236 mdh +1.8 15.0 malate dehydrogenase (NCBI) compare
b2217 rcsB +1.6 12.8 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b4154 frdA +1.5 13.8 fumarate reductase (NCBI) compare
b4152 frdC +1.5 8.4 fumarate reductase subunit C (NCBI) compare
b2741 rpoS +1.5 12.1 RNA polymerase sigma factor (NCBI) compare
b4151 frdD +1.5 6.6 fumarate reductase subunit D (NCBI) compare
b4153 frdB +1.5 11.1 fumarate reductase (anaerobic), Fe-S subunit (NCBI) compare
b1594 dgsA +1.3 6.6 DNA-binding transcriptional repressor (NCBI) compare
b2528 iscA +1.3 3.2 iron-sulfur cluster assembly protein (NCBI) compare
b2168 fruK +1.0 1.1 1-phosphofructokinase (NCBI) compare
b3261 fis +1.0 3.0 DNA-binding protein Fis (NCBI) compare
b3181 greA +1.0 6.8 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b1492 gadC +1.0 8.3 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b3438 gntR +1.0 6.0 DNA-binding transcriptional repressor (NCBI) compare
b1823 cspC +1.0 3.0 stress protein, member of the CspA-family (NCBI) compare
b4122 fumB +0.8 7.1 anaerobic class I fumarate hydratase (fumarase B) (NCBI) compare
b2279 nuoK +0.8 2.1 NADH dehydrogenase subunit K (NCBI) compare
b0955 ycbZ +0.8 6.9 predicted peptidase (NCBI) compare
b2934 cmtB +0.8 1.1 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1954 dsrA +0.8 3.5 regulatory antisense RNA (NCBI) compare
b1187 fadR +0.8 4.6 fatty acid metabolism regulator (NCBI) compare
b1480 sra +0.8 2.4 30S ribosomal subunit protein S22 (NCBI) compare
b3512 GadE +0.8 4.0 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b1658 purR +0.7 4.2 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b1628 rsxB +0.7 2.3 electron transport complex protein RnfB (NCBI) compare
b4124 dcuR +0.7 6.4 DNA-binding response regulator in two-component regulatory system with DcuS (NCBI) compare
b4133 cadC +0.7 6.5 DNA-binding transcriptional activator (NCBI) compare
b1160 elbA +0.7 1.2 hypothetical protein (NCBI) compare
b1936 intG +0.7 1.0 predicted defective phage integrase (pseudogene) (RefSeq) compare
b2286 nuoC +0.7 4.0 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b2814 metZ +0.7 1.8 tRNA-Met (NCBI) compare
b4125 dcuS +0.6 5.5 sensory histidine kinase in two-component regulatory system with DcuR, regulator of anaerobic fumarate respiration (NCBI) compare
b4222 ytfP +0.6 3.1 hypothetical protein (NCBI) compare
b1949 fliQ +0.6 3.1 flagellar biosynthesis protein Q (NCBI) compare
b1536 ydeI +0.6 1.1 hypothetical protein (NCBI) compare
b1824 yobF +0.6 2.4 hypothetical protein (NCBI) compare
b4595 yciY +0.6 1.7 hypothetical protein (RefSeq) compare
b1007 b1007 +0.6 1.6 orf, hypothetical protein (VIMSS) compare
b2642 yfjW +0.6 1.0 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b0661 miaB +0.6 4.8 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b2502 ppx +0.5 4.4 exopolyphosphatase (NCBI) compare
b1627 rsxA +0.5 3.1 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b2343 b2343 +0.5 1.3 orf, hypothetical protein (VIMSS) compare
b3934 cytR +0.5 4.5 DNA-binding transcriptional dual regulator (NCBI) compare
b1848 yebG +0.5 1.5 conserved protein regulated by LexA (NCBI) compare
b3194 yrbE +0.5 4.1 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1582 ynfA +0.5 2.3 hypothetical protein (NCBI) compare
b1992 cobS +0.5 2.4 cobalamin synthase (NCBI) compare
b3446 yrhB +0.5 3.4 hypothetical protein (NCBI) compare
b3495 uspA +0.5 4.7 universal stress global response regulator (NCBI) compare
b1482 osmC +0.5 1.4 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b3146 yraL +0.5 3.8 predicted methyltransferase (NCBI) compare
b4443 gcvB +0.5 0.3 GcvB sRNA gene divergent from gcvA (RefSeq) compare
b4071 nrfB +0.5 2.2 formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS) compare
b3672 ivbL +0.5 2.3 ilvB operon leader peptide (NCBI) compare
b1355 b1355 +0.5 2.0 orf, hypothetical protein (VIMSS) compare
b1243 oppA +0.5 3.8 oligopeptide transporter subunit (NCBI) compare
b2285 nuoE +0.5 1.4 NADH dehydrogenase subunit E (NCBI) compare
b3779 gpp +0.5 4.3 guanosine pentaphosphatase/exopolyphosphatase (NCBI) compare
b4651 yibW +0.5 0.3 no description compare
b1246 oppD +0.5 3.0 oligopeptide transporter ATP-binding component (NCBI) compare
b2659 b2659 +0.5 2.0 orf, hypothetical protein (VIMSS) compare
b3516 gadX +0.5 4.1 DNA-binding transcriptional dual regulator (NCBI) compare
b2278 nuoL +0.5 3.3 NADH dehydrogenase subunit L (NCBI) compare
b2195 ccmG +0.5 1.9 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b1976 b1976 +0.5 1.8 orf, hypothetical protein (VIMSS) compare
b2281 nuoI +0.5 1.6 NADH dehydrogenase subunit I (NCBI) compare
b4062 soxS +0.5 1.2 DNA-binding transcriptional dual regulator (NCBI) compare
b2010 dacD +0.4 2.4 penicillin binding protein 6b (VIMSS) compare
b2637 yfjT +0.4 1.4 CP4-57 prophage; predicted protein (NCBI) compare
b1259 yciG +0.4 0.9 orf, hypothetical protein (VIMSS) compare
b1269 rluB +0.4 2.3 23S rRNA pseudouridylate synthase (NCBI) compare
b4066 yjcF +0.4 0.3 hypothetical protein (NCBI) compare
b3807 cyaY +0.4 2.7 frataxin-like protein (NCBI) compare
b1380 ldhA +0.4 1.4 D-lactate dehydrogenase (NCBI) compare
b2845 yqeG +0.4 3.9 predicted transporter (NCBI) compare
b1493 gadB +0.4 0.7 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b1999 yeeP +0.4 2.9 putative histone (VIMSS) compare
b4431 rprA +0.4 1.5 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b1249 cls +0.4 2.8 cardiolipin synthetase (NCBI) compare
b4049 dusA +0.4 3.2 tRNA-dihydrouridine synthase A (RefSeq) compare
b1630 rsxD +0.4 2.4 electron transport complex protein RnfD (NCBI) compare
b2277 nuoM +0.4 2.0 NADH dehydrogenase subunit M (NCBI) compare
b3443 yrhA +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b3507 dctR +0.4 3.5 protein involved in metabolism of C4-dicarboxylates (Katherine Huang) compare
b4409 blr +0.4 2.0 beta-lactam resistance membrane protein (NCBI) compare
b2398 yfeC +0.4 1.6 orf, hypothetical protein (VIMSS) compare
b2911 ssrS +0.4 0.7 6S regulatory RNA (NCBI) compare
b1642 slyA +0.4 1.5 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b2280 nuoJ +0.4 2.3 NADH dehydrogenase subunit J (NCBI) compare
b2991 hybF +0.4 1.7 protein involved with the maturation of hydrogenases 1 and 2 (NCBI) compare
b2283 nuoG +0.4 2.5 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b4215 ytfI +0.4 0.9 hypothetical protein (NCBI) compare
b0502 ylbG +0.4 1.7 no description compare
b1777 b1777 +0.4 0.9 orf, hypothetical protein (VIMSS) compare
b1141 ymfG +0.4 1.6 e14 prophage; predicted excisionase (NCBI) compare
b1837 yebW +0.4 1.4 orf, hypothetical protein (VIMSS) compare
b1583 ynfB +0.4 1.7 hypothetical protein (NCBI) compare
b2229 b2229 +0.4 1.2 orf, hypothetical protein (VIMSS) compare
b1960 vsr +0.4 1.8 DNA mismatch endonuclease of very short patch repair (NCBI) compare
b1672 ydhW +0.4 1.4 hypothetical protein (NCBI) compare
b4041 plsB +0.4 1.1 glycerol-3-phosphate acyltransferase (VIMSS) compare
b0357 yaiN +0.4 0.8 putative alpha helix chain (VIMSS) compare
b1139 lit +0.4 2.2 e14 prophage; cell death peptidase, inhibitor of T4 late gene expression (NCBI) compare
b0303 ykgI +0.4 0.4 orf, hypothetical protein (VIMSS) compare
b1842 holE +0.4 1.1 DNA polymerase III, theta subunit (NCBI) compare
b1600 mdtJ +0.4 0.8 multidrug efflux system transporter (NCBI) compare
b0982 yccY +0.4 1.8 putative phosphatase (VIMSS) compare
b0616 citE +0.4 1.9 citrate lyase beta chain (acyl lyase subunit) (VIMSS) compare
b1562 hokD +0.4 1.5 Qin prophage; small toxic polypeptide (NCBI) compare
b1735 chbR +0.4 1.4 DNA-binding transcriptional dual regulator (NCBI) compare
b4692 oweE +0.3 0.9 no description compare
b1799 yeaT +0.3 1.9 putative transcriptional regulator LYSR-type (VIMSS) compare
b2204 napH +0.3 1.6 quinol dehydrogenase membrane component (NCBI) compare
b1607 ydgC +0.3 1.1 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b1448 yncA +0.3 1.3 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b3043 ygiL +0.3 2.0 predicted fimbrial-like adhesin protein (NCBI) compare
b2288 nuoA +0.3 1.5 NADH dehydrogenase I chain A (VIMSS) compare
b1040 csgD +0.3 0.8 DNA-binding transcriptional activator in two-component regulatory system (NCBI) compare
b2409 yfeR +0.3 1.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b3500 gor +0.3 0.8 glutathione reductase (NCBI) compare
b2725 hycA +0.3 1.4 regulator of the transcriptional regulator FhlA (NCBI) compare
b2897 ygfY +0.3 1.3 hypothetical protein (NCBI) compare
b2326 yfcM +0.3 1.6 hypothetical protein (NCBI) compare
b3414 gntY +0.3 2.1 predicted gluconate transport associated protein (NCBI) compare
b1344 ydaO +0.3 1.5 predicted C32 tRNA thiolase (NCBI) compare
b3555 yiaG +0.3 1.8 predicted transcriptional regulator (NCBI) compare
b0461 ybaJ +0.3 2.1 hypothetical protein (NCBI) compare
b4396 rob +0.3 2.6 DNA-binding transcriptional activator (NCBI) compare
b0980 appA +0.3 2.5 phosphoanhydride phosphorylase (NCBI) compare
b1640 ydhH +0.3 2.0 anhydro-N-acetylmuramic acid kinase (NCBI) compare
b2092 gatC +0.3 2.2 galactitol-specific enzyme IIC component of PTS (NCBI) compare
b1134 ymfB +0.3 1.3 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) compare
b3193 yrbD +0.3 2.7 predicted ABC-type organic solvent transporter (NCBI) compare
b3884 yihW +0.3 2.2 putative DEOR-type transcriptional regulator (VIMSS) compare
b4108 phnA +0.3 1.7 hypothetical protein (NCBI) compare
b0730 mngR +0.3 0.7 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b1520 b1520 +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b3195 yrbF +0.3 2.7 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b2346 vacJ +0.3 2.4 predicted lipoprotein (NCBI) compare
b3961 oxyR +0.3 2.3 DNA-binding transcriptional dual regulator (NCBI) compare
b1218 chaC +0.3 2.0 regulatory protein for cation transport (RefSeq) compare
b2096 gatY +0.3 1.7 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b4064 yjcD +0.3 2.7 predicted permease (NCBI) compare
b0535 fimZ +0.3 1.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b0388 aroL +0.3 2.2 shikimate kinase II (NCBI) compare
b4397 creA +0.3 1.6 hypothetical protein (NCBI) compare
b4653 yicT +0.3 0.8 no description compare
b0851 nfsA +0.3 1.9 nitroreductase A, NADPH-dependent, FMN-dependent (NCBI) compare
b4050 yjbO +0.3 1.9 orf, hypothetical protein (VIMSS) compare
b4546 ypeB +0.3 0.7 hypothetical protein (NCBI) compare
b0104 guaC +0.3 2.5 guanosine 5'-monophosphate oxidoreductase (NCBI) compare
b2759 ygcK +0.3 2.1 hypothetical protein (NCBI) compare
b3708 tnaA +0.3 2.9 tryptophanase (VIMSS) compare
b3149 yraO +0.3 2.2 DnaA initiator-associating factor for replication initiation (NCBI) compare
b1156 tfaE +0.3 1.7 e14 prophage; predicted tail fiber assembly protein (NCBI) compare
b1710 btuE +0.3 1.7 predicted glutathione peroxidase (NCBI) compare
b3468 yhhN +0.3 2.6 conserved inner membrane protein (NCBI) compare
b1333 uspE +0.3 1.3 stress-induced protein (NCBI) compare
b0931 pncB +0.3 2.0 nicotinate phosphoribosyltransferase (NCBI) compare
b2094 gatA +0.3 1.7 galactitol-specific enzyme IIA component of PTS (NCBI) compare
b1591 b1591 +0.3 1.0 putative oxidoreductase component (VIMSS) compare
b2829 ptsP +0.3 2.8 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b3166 truB +0.3 2.4 tRNA pseudouridine synthase B (NCBI) compare
b1725 yniA +0.3 1.9 predicted phosphotransferase/kinase (NCBI) compare
b0871 poxB +0.3 2.2 pyruvate dehydrogenase (NCBI) compare
b1674 ydhY +0.3 2.0 predicted 4Fe-4S ferridoxin-type protein (NCBI) compare
b1765 ydjA +0.3 0.4 predicted oxidoreductase (NCBI) compare
b4590 ybfK +0.3 0.4 hypothetical protein (RefSeq) compare
b2287 nuoB +0.3 1.3 NADH dehydrogenase subunit B (NCBI) compare
b3207 yrbL +0.3 1.7 hypothetical protein (NCBI) compare
b3643 rph +0.3 1.4 ribonuclease PH (NCBI) compare
b0485 ybaS +0.3 2.4 predicted glutaminase (NCBI) compare
b4100 phnH +0.3 1.2 carbon-phosphorus lyase complex subunit (NCBI) compare
b1465 narV +0.3 1.2 nitrate reductase 2 (NRZ), gamma subunit (NCBI) compare
b4282 yjhE +0.3 2.0 orf, hypothetical protein (VIMSS) compare
b0858 ybjO +0.3 1.2 predicted inner membrane protein (NCBI) compare
b4435 isrC +0.3 1.0 unknown RNA (NCBI) compare
b3834 yigP +0.3 2.1 hypothetical protein (NCBI) compare
b0736 ybgC +0.3 1.5 predicted acyl-CoA thioesterase (NCBI) compare
b1245 oppC +0.3 1.6 oligopeptide transporter subunit (NCBI) compare
b2582 trxC +0.3 1.0 thioredoxin 2 (NCBI) compare
b1400 paaY +0.3 1.1 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b1503 ydeR +0.3 1.2 predicted fimbrial-like adhesin protein (NCBI) compare
b2165 yeiN +0.3 1.5 hypothetical protein (NCBI) compare
b1530 marR +0.3 1.2 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b3341 rpsG +0.3 0.6 30S ribosomal protein S7 (NCBI) compare
b2643 yfjX +0.3 1.3 CP4-57 prophage; predicted antirestriction protein (NCBI) compare
b3191 yrbB +0.3 2.3 orf, hypothetical protein (VIMSS) compare
b3192 yrbC +0.3 2.3 predicted ABC-type organic solvent transporter (NCBI) compare
b3816 corA +0.3 2.2 magnesium/nickel/cobalt transporter (NCBI) compare
b3071 yqjI +0.3 1.6 predicted transcriptional regulator (NCBI) compare
b3890 yiiF +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b0786 ybhL +0.3 2.2 predicted inner membrane protein (NCBI) compare
b3228 sspB +0.3 1.5 ClpXP protease specificity-enhancing factor (NCBI) compare
b1548 nohA +0.3 0.9 Qin prophage; predicted packaging protein (RefSeq) compare
b2636 b2636 +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b2151 galS +0.3 2.2 DNA-binding transcriptional repressor (NCBI) compare
b3032 cpdA +0.3 2.1 cyclic 3',5'-adenosine monophosphate phosphodiesterase (NCBI) compare
b1183 umuD +0.3 0.6 DNA polymerase V, subunit D (NCBI) compare


Specific Phenotypes

For 10 genes in this experiment