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Experiment set3IT026 for
Methanococcus maripaludis S2
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H2 McCas, 5g/L bicarb, 40 psi H2CO2
200 most detrimental genes:
gene
name
fitness
t score
description
MMP
_RS03125
+1.2
7.2
cell division ATPase MinD
compare
MMP
_RS08860
+1.1
5.9
MinD/ParA family protein
compare
MMP
_RS08855
+1.1
7.2
hypothetical protein
compare
MMP
_RS01310
+1.1
9.0
hypothetical protein
compare
MMP
_RS00240
+1.1
8.1
type II secretion system F family protein
compare
MMP
_RS01290
+1.0
7.3
class III signal peptide-containing protein
compare
MMP
_RS06600
+1.0
1.8
class III signal peptide-containing protein
compare
MMP
_RS01305
+1.0
8.6
hypothetical protein
compare
MMP
_RS01315
+1.0
9.2
DUF2341 domain-containing protein
compare
MMP
_RS01295
+1.0
6.5
hypothetical protein
compare
MMP
_RS01280
+1.0
7.0
DUF515 domain-containing protein
compare
MMP
_RS07330
+1.0
9.1
membrane protein
compare
MMP
_RS01285
+1.0
6.6
hypothetical protein
compare
MMP
_RS00245
+1.0
8.1
type II secretion system F family protein
compare
MMP
_RS01270
+1.0
7.1
prepilin peptidase
compare
MMP
_RS09055
+0.9
1.5
CAP domain-containing protein
compare
MMP
_RS07840
+0.9
3.8
ribonuclease III
compare
MMP
_RS01275
+0.9
4.1
class III signal peptide-containing protein
compare
MMP
_RS08675
+0.8
2.9
class III signal peptide-containing protein
compare
MMP
_RS05635
+0.8
6.1
NAD-dependent epimerase/dehydratase family protein
compare
MMP
_RS06760
+0.8
3.5
hypothetical protein
compare
MMP
_RS02605
+0.8
0.9
MotA/TolQ/ExbB proton channel family protein
compare
MMP
_RS00250
+0.8
6.3
Flp pilus assembly complex ATPase component TadA
compare
MMP
_RS08275
+0.7
5.7
hypothetical protein
compare
MMP
_RS06090
+0.7
3.5
iron ABC transporter permease
compare
MMP
_RS01195
+0.7
1.3
metalloregulator ArsR/SmtB family transcription factor
compare
MMP
_RS04145
+0.7
1.8
hypothetical protein
compare
MMP
_RS06585
+0.7
1.8
DMT family protein
compare
MMP
_RS03220
+0.7
2.6
RNA 2' 3'-cyclic phosphodiesterase
compare
MMP
_RS05865
+0.7
2.4
hypothetical protein
compare
MMP
_RS06095
+0.6
4.2
ABC transporter ATP-binding protein
compare
MMP
_RS03965
+0.6
2.2
hypothetical protein
compare
MMP
_RS01055
+0.6
3.7
hypothetical protein
compare
MMP
_RS08775
+0.6
3.7
methanogenesis marker 9 domain-containing protein
compare
MMP
_RS01085
+0.6
1.7
hypothetical protein
compare
MMP
_RS01980
+0.6
4.9
trimeric intracellular cation channel family protein
compare
MMP
_RS08455
+0.6
1.7
multiprotein bridging factor aMBF1
compare
MMP
_RS08940
+0.6
1.2
MetS family NSS transporter small subunit
compare
MMP
_RS06035
+0.6
4.0
glycosyltransferase family 2 protein
compare
MMP
_RS01100
+0.6
1.3
ABC transporter ATP-binding protein
compare
MMP
_RS01125
+0.6
3.2
thioredoxin family protein
compare
MMP
_RS03320
+0.6
1.5
metal-dependent transcriptional regulator
compare
MMP
_RS03840
+0.6
3.1
hypothetical protein
compare
MMP
_RS06525
+0.6
1.0
YciI family protein
compare
MMP
_RS02965
+0.5
3.4
carboxymuconolactone decarboxylase family protein
compare
MMP
_RS05580
+0.5
4.6
glycosyltransferase
compare
MMP
_RS06910
+0.5
3.1
RlmF-related methyltransferase
compare
MMP
_RS04410
+0.5
3.1
hypothetical protein
compare
MMP
_RS05095
+0.5
2.6
CO dehydrogenase/acetyl-CoA synthase complex subunit epsilon
compare
MMP
_RS00990
+0.5
2.1
hypothetical protein
compare
MMP
_RS05985
+0.5
1.5
hypothetical protein
compare
MMP
_RS03300
+0.5
0.6
TOBE domain-containing protein
compare
MMP
_RS08130
+0.5
3.4
IGHMBP2 family helicase
compare
MMP
_RS07880
+0.5
2.9
AAA family ATPase
compare
MMP
_RS06050
+0.5
3.1
ZIP family metal transporter
compare
MMP
_RS05965
+0.5
2.1
carboxymuconolactone decarboxylase family protein
compare
MMP
_RS08640
+0.5
3.4
TIM barrel protein
compare
MMP
_RS00395
+0.5
1.2
MGMT family protein
compare
MMP
_RS00570
+0.5
1.2
protein-L-isoaspartate O-methyltransferase
compare
MMP
_RS06065
+0.5
4.2
iron ABC transporter substrate-binding protein
compare
MMP
_RS02540
+0.5
2.9
DUF2283 domain-containing protein
compare
MMP
_RS04610
+0.5
2.5
Dabb family protein
compare
MMP
_RS08400
+0.5
1.4
MJ0307 family thioredoxin
compare
MMP
_RS05085
+0.5
2.8
AAA family ATPase
compare
MMP
_RS00290
+0.5
3.7
hypothetical protein
compare
MMP
_RS08410
+0.4
1.6
DUF169 domain-containing protein
compare
MMP
_RS06990
+0.4
2.1
CatB-related O-acetyltransferase
compare
MMP
_RS08580
+0.4
2.3
flagellin
compare
MMP
_RS00140
+0.4
2.0
DUF192 domain-containing protein
compare
MMP
_RS07550
+0.4
2.6
L-serine ammonia-lyase iron-sulfur-dependent subunit alpha
compare
MMP
_RS01660
+0.4
1.9
hypothetical protein
compare
MMP
_RS01870
+0.4
2.5
acyl-CoA dehydratase activase
compare
MMP
_RS06680
+0.4
3.0
Coenzyme F420 hydrogenase/dehydrogenase beta subunit C-terminal domain
compare
MMP
_RS07770
+0.4
2.4
alanine racemase
compare
MMP
_RS09025
+0.4
0.7
hypothetical protein
compare
MMP
_RS06935
+0.4
2.3
protease complex subunit PrcB family protein
compare
MMP
_RS09045
+0.4
2.9
class III signal peptide-containing protein
compare
MMP
_RS05090
+0.4
2.4
CO dehydrogenase/CO-methylating acetyl-CoA synthase complex subunit beta
compare
MMP
_RS03625
+0.4
2.4
cell wall-binding protein
compare
MMP
_RS08265
+0.4
1.8
rubrerythrin
compare
MMP
_RS03065
+0.4
1.2
AzlC family ABC transporter permease
compare
MMP
_RS08080
+0.4
2.2
YchF/TatD family DNA exonuclease
compare
MMP
_RS02915
+0.4
2.3
amino acid ABC transporter permease
compare
MMP
_RS07800
+0.4
2.2
ATP-binding cassette domain-containing protein
compare
MMP
_RS04060
+0.4
2.0
hypothetical protein
compare
MMP
_RS08615
+0.4
0.6
flagellar protein G
compare
MMP
_RS07775
+0.4
1.4
alanine dehydrogenase
compare
MMP
_RS02830
+0.4
1.9
DUF432 domain-containing protein
compare
MMP
_RS08930
+0.4
1.0
hypothetical protein
compare
MMP
_RS00670
+0.4
1.4
TIM barrel protein
compare
MMP
_RS07890
+0.4
1.1
OB-fold nucleic acid binding domain-containing protein
compare
MMP
_RS06560
+0.4
2.2
hypothetical protein
compare
MMP
_RS02020
+0.4
1.4
gamma-glutamylcyclotransferase
compare
MMP
_RS03080
+0.4
0.6
universal stress protein
compare
MMP
_RS02745
+0.4
1.0
hypothetical protein
compare
MMP
_RS06700
+0.4
2.4
hypothetical protein
compare
MMP
_RS07895
+0.4
1.1
cytochrome c
compare
MMP
_RS03090
+0.4
2.1
cation:proton antiporter
compare
MMP
_RS06115
+0.3
2.5
class II aldolase/adducin family protein
compare
MMP
_RS05100
+0.3
3.0
CO dehydrogenase/acetyl-CoA synthase complex subunit alpha
compare
MMP
_RS02545
+0.3
1.5
hypothetical protein
compare
MMP
_RS05785
+0.3
2.5
ATP-binding protein
compare
MMP
_RS05075
+0.3
2.8
acetyl-CoA decarbonylase/synthase complex subunit gamma
compare
MMP
_RS08090
+0.3
1.8
hypothetical protein
compare
MMP
_RS02595
+0.3
0.7
putative zinc-binding protein
compare
MMP
_RS01460
+0.3
1.8
hypothetical protein
compare
MMP
_RS07100
+0.3
2.6
hypothetical protein
compare
MMP
_RS09030
+0.3
1.5
hypothetical protein
compare
MMP
_RS06545
+0.3
1.7
ferredoxin family protein
compare
MMP
_RS07590
+0.3
2.2
hypothetical protein
compare
MMP
_RS03900
+0.3
1.6
minichromosome maintenance protein MCM
compare
MMP
_RS01075
+0.3
0.6
hypothetical protein
compare
MMP
_RS06735
+0.3
1.6
class I SAM-dependent rRNA methyltransferase
compare
MMP
_RS06335
+0.3
1.2
HAD family hydrolase
compare
MMP
_RS02675
+0.3
0.7
hypothetical protein
compare
MMP
_RS01010
+0.3
2.3
DUF4352 domain-containing protein
compare
MMP
_RS06550
+0.3
1.7
AMP-binding protein
compare
MMP
_RS05970
+0.3
1.2
class II SORL domain-containing protein
compare
MMP
_RS06965
+0.3
1.8
TIGR04014 family B12-binding domain/radical SAM domain-containing protein
compare
MMP
_RS00660
+0.3
2.3
alkaline phosphatase family protein
compare
MMP
_RS00480
+0.3
1.5
hypothetical protein
compare
MMP
_RS05140
+0.3
1.2
helix-turn-helix transcriptional regulator
compare
MMP
_RS08395
+0.3
1.5
DsrE family protein
compare
MMP
_RS01490
+0.3
1.7
tetratricopeptide repeat protein
compare
MMP
_RS02390
+0.3
1.9
NifB/NifX family molybdenum-iron cluster-binding protein
compare
MMP
_RS08610
+0.3
2.0
flagellar protein F
compare
MMP
_RS02625
+0.3
1.5
ribonuclease H-like domain-containing protein
compare
MMP
_RS00965
+0.3
0.8
hypothetical protein
compare
MMP
_RS07815
+0.3
0.5
DUF2120 domain-containing protein
compare
MMP
_RS02025
+0.3
2.4
DNA-directed DNA polymerase
compare
MMP
_RS00090
+0.3
1.5
CoA-binding protein
compare
MMP
_RS01135
+0.3
2.2
molybdate ABC transporter substrate-binding protein
compare
MMP
_RS06320
+0.3
1.5
hypothetical protein
compare
MMP
_RS07910
+0.3
1.8
NAD(P)H-hydrate dehydratase
compare
MMP
_RS08380
+0.3
1.3
phosphatase PAP2 family protein
compare
MMP
_RS07345
+0.3
1.3
glycine synthesis protein GlyXL
(from data)
compare
MMP
_RS06465
+0.3
2.0
NERD domain-containing protein
compare
MMP
_RS07155
+0.3
1.6
MFS transporter
compare
MMP
_RS07085
+0.3
1.9
ribonuclease HII
compare
MMP
_RS02425
+0.3
1.5
hypothetical protein
compare
MMP
_RS01060
+0.3
2.1
calcium/sodium antiporter
compare
MMP
_RS03490
+0.3
0.7
DUF2115 domain-containing protein
compare
MMP
_RS04670
+0.3
2.0
selenouridine synthase SelU-like subunit
compare
MMP
_RS07805
+0.3
1.1
ABC transporter permease
compare
MMP
_RS05715
+0.3
1.4
hypothetical protein
compare
MMP
_RS06605
+0.3
1.8
DUF5814 domain-containing protein
compare
MMP
_RS06010
+0.3
2.0
heavy metal translocating P-type ATPase
compare
MMP
_RS08750
+0.3
0.5
hypothetical protein
compare
MMP
_RS04205
+0.3
1.6
carboxymuconolactone decarboxylase family protein
compare
MMP
_RS08250
+0.3
1.7
pyruvate kinase
compare
MMP
_RS01155
+0.3
1.4
XRE family transcriptional regulator
compare
MMP
_RS01300
+0.3
2.1
hypothetical protein
compare
MMP
_RS03745
+0.3
1.7
phenylacetate--CoA ligase
compare
MMP
_RS00665
+0.3
1.1
hypothetical protein
compare
MMP
_RS06375
+0.3
1.4
ATP-dependent sacrificial sulfur transferase LarE
compare
MMP
_RS05685
+0.3
1.5
winged helix-turn-helix domain-containing protein
compare
MMP
_RS07685
+0.3
1.6
TIGR01212 family radical SAM protein
compare
MMP
_RS03550
+0.3
0.7
DUF2124 domain-containing protein
compare
MMP
_RS04730
+0.3
2.0
flavodoxin family protein
compare
MMP
_RS08845
+0.3
1.5
archaellum operon transcriptional activator EarA family protein
compare
MMP
_RS04020
+0.3
1.2
hypothetical protein
compare
MMP
_RS08770
+0.3
1.4
hypothetical protein
compare
MMP
_RS00700
+0.3
1.5
OB-fold nucleic acid binding domain-containing protein
compare
MMP
_RS01145
+0.2
1.3
ABC transporter ATP-binding protein
compare
MMP
_RS02305
+0.2
2.0
DUF378 domain-containing protein
compare
MMP
_RS08835
+0.2
1.3
H(2)-dependent methylenetetrahydromethanopterin dehydrogenase-related protein
compare
MMP
_RS00795
+0.2
1.7
formate dehydrogenase H subunit alpha selenocysteine-containing
compare
MMP
_RS02700
+0.2
1.3
formylmethanofuran dehydrogenase subunit C
compare
MMP
_RS01635
+0.2
1.2
thiamine pyrophosphate-dependent enzyme
compare
MMP
_RS02430
+0.2
1.8
hypothetical protein
compare
MMP
_RS01790
+0.2
1.3
restriction endonuclease
compare
MMP
_RS08490
+0.2
2.1
hypothetical protein
compare
MMP
_RS02295
+0.2
1.7
TatD family hydrolase
compare
MMP
_RS08285
+0.2
1.4
serine/threonine protein kinase
compare
MMP
_RS03440
+0.2
0.4
MoaD/ThiS family protein
compare
MMP
_RS06745
+0.2
1.0
DUF2119 domain-containing protein
compare
MMP
_RS08575
+0.2
2.0
hypothetical protein
compare
MMP
_RS02005
+0.2
1.5
RNA ligase
compare
MMP
_RS06100
+0.2
1.6
GNAT family N-acetyltransferase
compare
MMP
_RS07905
+0.2
0.9
DUF3343 domain-containing protein
compare
MMP
_RS06365
+0.2
0.8
hypothetical protein
compare
MMP
_RS07115
+0.2
1.3
MBL fold metallo-hydrolase
compare
MMP
_RS01095
+0.2
1.0
iron ABC transporter permease
compare
MMP
_RS04190
+0.2
1.0
hypothetical protein
compare
MMP
_RS05080
+0.2
1.8
acetyl-CoA decarbonylase/synthase complex subunit delta
compare
MMP
_RS06175
+0.2
1.4
winged helix-turn-helix transcriptional regulator
compare
MMP
_RS06710
+0.2
1.0
selenium-binding protein
compare
MMP
_RS03950
+0.2
1.4
HNH endonuclease
compare
MMP
_RS03740
+0.2
1.5
indolepyruvate oxidoreductase subunit beta
compare
MMP
_RS04285
+0.2
1.4
Ni/Fe hydrogenase subunit alpha
compare
MMP
_RS07185
+0.2
1.6
pyridoxal phosphate-dependent aminotransferase
compare
MMP
_RS01640
+0.2
1.3
2-oxoacid:acceptor oxidoreductase subunit alpha
compare
MMP
_RS02290
+0.2
1.2
MBL fold metallo-hydrolase
compare
MMP
_RS01685
+0.2
0.6
indolepyruvate oxidoreductase subunit beta
compare
MMP
_RS05065
+0.2
0.8
CooT family nickel-binding protein
compare
MMP
_RS02385
+0.2
1.6
ATP-binding protein
compare
MMP
_RS08315
+0.2
0.9
S-methyl-5-thioribose-1-phosphate isomerase
compare
MMP
_RS06540
+0.2
0.5
3-methyl-2-oxobutanoate dehydrogenase subunit VorB
compare
MMP
_RS01130
+0.2
1.4
flavoprotein
compare
MMP
_RS02490
+0.2
1.1
hypothetical protein
compare
Specific Phenotypes
None in this experiment
For Methanococcus maripaludis S2 in rich t1 experiments