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Experiment set2IT087 for
Methanococcus maripaludis JJ
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H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
200 most detrimental genes:
gene
name
fitness
t score
description
MMJJ
_RS04415
+1.2
3.5
hypothetical protein
compare
MMJJ
_RS08720
+0.9
7.5
UDP-glucose/GDP-mannose dehydrogenase family protein
compare
MMJJ
_RS08730
+0.9
7.2
glycosyltransferase family 4 protein
compare
MMJJ
_RS04720
+0.8
1.8
IMP dehydrogenase
compare
MMJJ
_RS08760
+0.8
6.1
glycosyltransferase family 9 protein
compare
MMJJ
_RS08025
+0.8
3.2
hypothetical protein
compare
MMJJ
_RS08750
+0.8
6.5
glycosyltransferase
compare
MMJJ
_RS08775
+0.7
5.5
PfkB family carbohydrate kinase
compare
MMJJ
_RS08780
+0.7
4.1
D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase
compare
MMJJ
_RS08765
+0.7
5.6
glycosyltransferase family 9 protein
compare
MMJJ
_RS09295
+0.6
0.9
hypothetical protein
compare
MMJJ
_RS06975
+0.6
1.9
protein translocase SEC61 complex subunit gamma
compare
MMJJ
_RS00420
+0.6
3.8
CBS domain-containing protein
compare
MMJJ
_RS01210
+0.6
2.8
hypothetical protein
compare
MMJJ
_RS07030
+0.6
5.3
hypothetical protein
compare
MMJJ
_RS03205
+0.6
4.2
metallophosphoesterase
compare
MMJJ
_RS07035
+0.6
0.8
glycosyl transferase
compare
MMJJ
_RS08725
+0.6
4.3
flippase
compare
MMJJ
_RS08715
+0.6
3.6
NAD-dependent epimerase/dehydratase family protein
compare
MMJJ
_RS08040
+0.5
4.3
NAD(P)/FAD-dependent oxidoreductase
compare
MMJJ
_RS09390
+0.5
1.6
hypothetical protein
compare
MMJJ
_RS07280
+0.5
3.6
phosphomannomutase/phosphoglucomutase
compare
MMJJ
_RS03410
+0.5
2.0
hypothetical protein
compare
MMJJ
_RS08785
+0.5
3.1
D-sedoheptulose 7-phosphate isomerase
compare
MMJJ
_RS07680
+0.5
3.6
glucose-6-phosphate isomerase
compare
MMJJ
_RS00415
+0.4
2.5
DedA family protein
compare
MMJJ
_RS01125
+0.4
2.3
hypothetical protein
compare
MMJJ
_RS05980
+0.4
2.8
DUF5400 domain-containing protein
compare
MMJJ
_RS08830
+0.4
1.9
hypothetical protein
compare
MMJJ
_RS01010
+0.4
3.5
right-handed parallel beta-helix repeat-containing protein
compare
MMJJ
_RS03360
+0.4
0.7
hypothetical protein
compare
MMJJ
_RS04215
+0.3
3.1
DMT family transporter
compare
MMJJ
_RS09325
+0.3
2.0
hypothetical protein
compare
MMJJ
_RS08710
+0.3
2.5
UTP--glucose-1-phosphate uridylyltransferase GalU
compare
MMJJ
_RS06800
+0.3
2.1
prefoldin subunit alpha
compare
MMJJ
_RS05115
+0.3
1.4
P-II family nitrogen regulator
compare
MMJJ
_RS00480
+0.3
2.6
flippase
compare
MMJJ
_RS06720
+0.3
2.7
mechanosensitive ion channel family protein
compare
MMJJ
_RS06210
+0.3
2.3
hypothetical protein
compare
MMJJ
_RS04480
+0.3
1.1
hypothetical protein
compare
MMJJ
_RS06130
+0.3
1.5
hypothetical protein
compare
MMJJ
_RS03605
+0.3
1.8
TIGR00297 family protein
compare
MMJJ
_RS09300
+0.3
1.1
hypothetical protein
compare
MMJJ
_RS06430
+0.3
2.0
haloacid dehalogenase
compare
MMJJ
_RS08515
+0.3
1.0
secondary thiamine-phosphate synthase enzyme YjbQ
compare
MMJJ
_RS05510
+0.3
2.3
archaellum operon transcriptional activator EarA family protein
compare
MMJJ
_RS01615
+0.3
1.3
MoaD/ThiS family protein
compare
MMJJ
_RS09065
+0.3
1.7
hypothetical protein
compare
MMJJ
_RS06205
+0.3
2.1
EamA family transporter
compare
MMJJ
_RS06275
+0.3
1.7
hypothetical protein
compare
MMJJ
_RS05870
+0.3
1.3
pyridoxal 5'-phosphate synthase glutaminase subunit PdxT
compare
MMJJ
_RS07690
+0.3
1.9
glycosyltransferase family 4 protein
compare
MMJJ
_RS03370
+0.3
1.1
hypothetical protein
compare
MMJJ
_RS01035
+0.3
1.3
hypothetical protein
compare
MMJJ
_RS05760
+0.3
2.4
flagellar protein F
compare
MMJJ
_RS07210
+0.3
2.0
AmmeMemoRadiSam system protein B
compare
MMJJ
_RS08705
+0.3
1.9
AEC family transporter
compare
MMJJ
_RS01825
+0.3
1.5
DNA repair and recombination protein RadB
compare
MMJJ
_RS06770
+0.3
2.1
hypothetical protein
compare
MMJJ
_RS06465
+0.2
1.8
hypothetical protein
compare
MMJJ
_RS04585
+0.2
1.7
phenylacetate--CoA ligase
compare
MMJJ
_RS07665
+0.2
2.3
formate dehydrogenase subunit alpha
compare
MMJJ
_RS08930
+0.2
1.9
5'/3'-nucleotidase SurE
compare
MMJJ
_RS05740
+0.2
2.1
archaellar assembly protein FlaJ
compare
MMJJ
_RS09370
+0.2
1.0
heavy-metal-associated domain-containing protein
compare
MMJJ
_RS06080
+0.2
2.0
serine/threonine protein kinase
compare
MMJJ
_RS05365
+0.2
1.5
DUF2798 domain-containing protein
compare
MMJJ
_RS06280
+0.2
2.0
hypothetical protein
compare
MMJJ
_RS06810
+0.2
2.0
L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha
compare
MMJJ
_RS08370
+0.2
1.2
hypothetical protein
compare
MMJJ
_RS05040
+0.2
1.7
7-carboxy-7-deazaguanine synthase QueE
compare
MMJJ
_RS08615
+0.2
1.5
oligosaccharide repeat unit polymerase family protein
compare
MMJJ
_RS05505
+0.2
2.2
hypothetical protein
compare
MMJJ
_RS07645
+0.2
1.6
selenium-binding protein
compare
MMJJ
_RS03510
+0.2
1.5
acyl-CoA dehydratase activase
compare
MMJJ
_RS05195
+0.2
1.0
hypothetical protein
compare
MMJJ
_RS05250
+0.2
1.4
HDIG domain-containing protein
compare
MMJJ
_RS06105
+0.2
1.6
DUF99 family protein
compare
MMJJ
_RS06470
+0.2
1.5
tripartite tricarboxylate transporter permease
compare
MMJJ
_RS06120
+0.2
1.6
DUF4013 domain-containing protein
compare
MMJJ
_RS07420
+0.2
1.5
protease complex subunit PrcB family protein
compare
MMJJ
_RS07610
+0.2
1.3
DUF2119 domain-containing protein
compare
MMJJ
_RS03045
+0.2
1.4
hypothetical protein
compare
MMJJ
_RS05190
+0.2
1.3
gamma carbonic anhydrase family protein
compare
MMJJ
_RS05820
+0.2
1.1
DUF2304 domain-containing protein
compare
MMJJ
_RS02295
+0.2
1.9
hypothetical protein
compare
MMJJ
_RS09010
+0.2
1.0
23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH
compare
MMJJ
_RS05975
+0.2
1.4
sulfurtransferase TusA family protein
compare
MMJJ
_RS06755
+0.2
1.6
diadenylate cyclase
compare
MMJJ
_RS06295
+0.2
1.3
DUF2096 family protein
compare
MMJJ
_RS05745
+0.2
1.7
type II/IV secretion system ATPase subunit
compare
MMJJ
_RS09020
+0.2
1.0
hypothetical protein
compare
MMJJ
_RS06520
+0.2
1.7
TldD/PmbA family protein
compare
MMJJ
_RS06765
+0.2
1.7
hypothetical protein
compare
MMJJ
_RS06950
+0.2
1.4
4-demethylwyosine synthase TYW1
compare
MMJJ
_RS05750
+0.2
1.5
flagellar accessory protein FlaH
compare
MMJJ
_RS00870
+0.2
1.3
F420-nonreducing hydrogenase
compare
MMJJ
_RS06190
+0.2
1.6
7-cyano-7-deazaguanine synthase
compare
MMJJ
_RS09255
+0.2
0.9
hypothetical protein
compare
MMJJ
_RS07255
+0.2
1.7
hypothetical protein
compare
MMJJ
_RS05785
+0.2
1.3
flagellin
compare
MMJJ
_RS04405
+0.2
1.0
hypothetical protein
compare
MMJJ
_RS03690
+0.2
1.2
indolepyruvate oxidoreductase subunit beta
compare
MMJJ
_RS01175
+0.2
1.5
hypothetical protein
compare
MMJJ
_RS03530
+0.2
1.5
phosphate ABC transporter substrate-binding protein
compare
MMJJ
_RS04135
+0.2
1.0
7-cyano-7-deazaguanine synthase QueC
compare
MMJJ
_RS01920
+0.2
1.4
phosphoglycerate mutase
compare
MMJJ
_RS01760
+0.2
1.1
N-acetyltransferase
compare
MMJJ
_RS03385
+0.2
1.1
hypothetical protein
compare
MMJJ
_RS04305
+0.2
1.6
right-handed parallel beta-helix repeat-containing protein
compare
MMJJ
_RS05755
+0.2
1.4
flagellar protein G
compare
MMJJ
_RS01405
+0.2
0.3
proline--tRNA ligase
compare
MMJJ
_RS06585
+0.2
1.4
alanine racemase
compare
MMJJ
_RS07385
+0.2
1.3
ribosomal protein S18-alanine N-acetyltransferase
compare
MMJJ
_RS05035
+0.2
1.1
4Fe-4S binding protein
compare
MMJJ
_RS06440
+0.2
1.2
DUF4870 domain-containing protein
compare
MMJJ
_RS05200
+0.2
1.4
TIGR04013 family B12-binding domain/radical SAM domain-containing protein
compare
MMJJ
_RS03460
+0.2
1.3
hypothetical protein
compare
MMJJ
_RS06740
+0.2
1.0
energy-coupling factor ABC transporter substrate-binding protein
compare
MMJJ
_RS05925
+0.2
1.2
DUF2099 family protein
compare
MMJJ
_RS06055
+0.2
1.1
S-methyl-5-thioribose-1-phosphate isomerase
compare
MMJJ
_RS06945
+0.2
1.2
molybdopterin-guanine dinucleotide biosynthesis protein MobB
compare
MMJJ
_RS05335
+0.2
0.8
heavy metal-binding domain-containing protein
compare
MMJJ
_RS07445
+0.2
1.0
RlmF-related methyltransferase
compare
MMJJ
_RS00930
+0.2
0.9
queuosine precursor transporter
compare
MMJJ
_RS06135
+0.2
1.2
50S ribosomal protein L11 methyltransferase
compare
MMJJ
_RS05605
+0.2
1.0
hypothetical protein
compare
MMJJ
_RS04270
+0.2
0.9
DUF364 domain-containing protein
compare
MMJJ
_RS02245
+0.2
1.0
DUF432 domain-containing protein
compare
MMJJ
_RS05700
+0.2
1.1
metallophosphoesterase
compare
MMJJ
_RS01060
+0.2
0.9
ABC transporter ATP-binding protein
compare
MMJJ
_RS07865
+0.2
1.2
zinc ribbon domain-containing protein
compare
MMJJ
_RS00965
+0.2
0.9
cyclophilin-like fold protein
compare
MMJJ
_RS07920
+0.2
1.2
6-carboxytetrahydropterin synthase QueD
compare
MMJJ
_RS05730
+0.2
1.3
TIM barrel protein
compare
MMJJ
_RS07670
+0.2
1.3
Coenzyme F420 hydrogenase/dehydrogenase, beta subunit C-terminal domain
compare
MMJJ
_RS08365
+0.1
0.7
DsrE family protein
compare
MMJJ
_RS06710
+0.1
1.3
TldD/PmbA family protein
compare
MMJJ
_RS05235
+0.1
1.4
Flp pilus assembly complex ATPase component TadA
compare
MMJJ
_RS06085
+0.1
0.8
proteasome assembly chaperone family protein
compare
MMJJ
_RS08630
+0.1
1.0
signal recognition particle subunit SRP19/SEC65 family protein
compare
MMJJ
_RS05585
+0.1
1.0
hypothetical protein
compare
MMJJ
_RS05095
+0.1
1.3
ammonium transporter
compare
MMJJ
_RS08310
+0.1
0.9
ZIP family metal transporter
compare
MMJJ
_RS02340
+0.1
0.9
SAM-dependent methyltransferase
compare
MMJJ
_RS01110
+0.1
0.6
hypothetical protein
compare
MMJJ
_RS06445
+0.1
1.3
hypothetical protein
compare
MMJJ
_RS06460
+0.1
1.0
cytochrome c
compare
MMJJ
_RS04845
+0.1
1.2
ABC transporter substrate-binding protein
compare
MMJJ
_RS01265
+0.1
0.7
cupin domain-containing protein
compare
MMJJ
_RS01395
+0.1
0.8
DUF1894 domain-containing protein
compare
MMJJ
_RS05790
+0.1
1.0
flagellin
compare
MMJJ
_RS08445
+0.1
0.8
cell division ATPase MinD
compare
MMJJ
_RS04300
+0.1
0.9
hypothetical protein
compare
MMJJ
_RS04240
+0.1
1.1
molybdate ABC transporter substrate-binding protein
compare
MMJJ
_RS05890
+0.1
1.1
molybdate ABC transporter substrate-binding protein
compare
MMJJ
_RS05400
+0.1
0.6
CoA-binding protein
compare
MMJJ
_RS06515
+0.1
1.1
ribonuclease III
compare
MMJJ
_RS06160
+0.1
1.1
stage II sporulation protein M
compare
MMJJ
_RS04785
+0.1
0.9
OB-fold nucleic acid binding domain-containing protein
compare
MMJJ
_RS03015
+0.1
1.1
Ig-like domain-containing protein
compare
MMJJ
_RS06550
+0.1
1.0
ABC transporter permease
compare
MMJJ
_RS05690
+0.1
1.2
hypothetical protein
compare
MMJJ
_RS08810
+0.1
0.2
DUF4040 domain-containing protein
compare
MMJJ
_RS06045
+0.1
0.9
hypothetical protein
compare
MMJJ
_RS04625
+0.1
0.9
SLC13 family permease
compare
MMJJ
_RS06960
+0.1
1.1
CAP domain-containing protein
compare
MMJJ
_RS05290
+0.1
1.1
DUF4013 domain-containing protein
compare
MMJJ
_RS04690
+0.1
1.0
Coenzyme F420 hydrogenase/dehydrogenase, beta subunit C-terminal domain
compare
MMJJ
_RS08400
+0.1
0.6
CoB--CoM heterodisulfide reductase subunit C
compare
MMJJ
_RS09050
+0.1
0.4
hypothetical protein
compare
MMJJ
_RS04055
+0.1
0.6
hypothetical protein
compare
MMJJ
_RS08580
+0.1
0.5
YkgJ family cysteine cluster protein
compare
MMJJ
_RS02115
+0.1
0.9
class I SAM-dependent methyltransferase family protein
compare
MMJJ
_RS07365
+0.1
0.9
CatB-related O-acetyltransferase
compare
MMJJ
_RS02075
+0.1
0.5
acyl-CoA thioesterase
compare
MMJJ
_RS07740
+0.1
0.8
DUF2334 domain-containing protein
compare
MMJJ
_RS09005
+0.1
1.1
N-6 DNA methylase
compare
MMJJ
_RS07915
+0.1
0.8
CBS domain-containing protein
compare
MMJJ
_RS08075
+0.1
1.0
ATP-dependent DNA helicase
compare
MMJJ
_RS07835
+0.1
0.9
UPF0489 family protein
compare
MMJJ
_RS09215
+0.1
1.0
right-handed parallel beta-helix repeat-containing protein
compare
MMJJ
_RS04950
+0.1
1.0
TIGR00375 family protein
compare
MMJJ
_RS05695
+0.1
0.7
class III signal peptide-containing protein
compare
MMJJ
_RS05405
+0.1
0.9
DNA cytosine methyltransferase
compare
MMJJ
_RS09155
+0.1
0.9
RluA family pseudouridine synthase
compare
MMJJ
_RS06555
+0.1
1.0
ATP-binding cassette domain-containing protein
compare
MMJJ
_RS07480
+0.1
1.0
FAD-dependent oxidoreductase
compare
MMJJ
_RS04165
+0.1
0.9
sodium:solute symporter
compare
MMJJ
_RS02310
+0.1
0.8
ABC transporter ATP-binding protein
compare
MMJJ
_RS03350
+0.1
0.3
hypothetical protein
compare
MMJJ
_RS08345
+0.1
0.9
flavodoxin family protein
compare
MMJJ
_RS06685
+0.1
1.0
S-layer protein
compare
MMJJ
_RS04295
+0.1
0.9
DUF2202 domain-containing protein
compare
MMJJ
_RS04395
+0.1
0.9
hypothetical protein
compare
MMJJ
_RS04925
+0.1
0.9
cation:proton antiporter
compare
MMJJ
_RS06450
+0.1
0.8
DUF3343 domain-containing protein
compare
MMJJ
_RS03185
+0.1
0.2
alanine--tRNA ligase
compare
MMJJ
_RS00135
+0.1
0.7
FmdE family protein
compare
MMJJ
_RS04510
+0.1
0.9
hypothetical protein
compare
Specific Phenotypes
None in this experiment
For Methanococcus maripaludis JJ in rich t1 experiments