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Experiment set2IT059 for
Sinorhizobium meliloti 1021
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LB
200 most detrimental genes:
gene
name
fitness
t score
description
SMc03069
+7.3
20.7
6-phosphogluconolactonase
compare
SMc02659
+7.1
12.4
GTP pyrophosphokinase (ATP:GTP 3'-pyrophosphotransferase) protein
compare
SMc04003
+6.9
17.0
50S ribosomal protein L36
compare
SMc00647
+6.9
28.4
ribosomal large subunit pseudouridine synthase
compare
SMc00131
+6.4
44.5
transcriptional regulator
compare
SMc00349
+6.0
34.4
GTP-binding protein LepA
compare
SMc02112
+5.9
28.2
hypothetical protein
compare
SMc00711
+5.7
35.8
cyclohexadienyl dehydrogenase
compare
SMc00565
+5.4
15.3
50S ribosomal protein L9
compare
SMc02137
+5.3
26.5
ornithine carbamoyltransferase
compare
SMc03900
+5.3
35.9
cyclic beta-1,2-glucan ABc transporter
compare
SMc01099
+5.3
13.8
tRNA pseudouridine synthase A
compare
SMc02695
+5.2
32.5
GTP-dependent nucleic acid-binding protein EngD
compare
SMc04382
+5.1
36.9
beta-(1,2)-glucan production associated transmembrane protein
compare
SMc01124
+5.0
12.0
PII uridylyl-transferase
compare
SMc00566
+4.9
9.8
hypothetical protein
compare
SMc00419
+4.9
16.3
glutathione synthetase
compare
SMc03826
+4.8
16.3
argininosuccinate synthase
compare
SMc01206
+4.6
12.6
queuine tRNA-ribosyltransferase
compare
SMc00458
+4.4
36.2
transcriptional regulator
compare
SMc01275
+4.4
10.4
ribosomal large subunit pseudouridine synthase C protein
compare
SMc02558
+4.3
15.6
hypothetical protein
compare
SMc03855
+4.3
16.6
hypothetical protein
compare
SMc00495
+4.2
12.7
phosphoribosylaminoimidazolesuccinocarboxamide synthase
compare
SMc02503
+4.1
12.1
hypothetical protein
compare
SMc04094
+4.0
13.9
hypothetical protein
compare
SMa2020
+3.9
11.4
Transcriptional regulator
compare
SMc03863
+3.7
4.2
50S ribosomal protein L19
compare
SMc02050
+3.7
16.8
trigger factor
compare
SMc02367
+3.7
25.9
sensor histidine kinase transmembrane protein
compare
SMc00150
+3.6
33.2
hypothetical protein
compare
SMc01207
+3.5
13.9
S-adenosylmethionine--tRNA ribosyltransferase-isomerase
compare
SMc02365
+3.5
12.2
protease precursor protein
compare
SMc01215
+3.4
17.9
carbamoyl phosphate synthase large subunit
compare
SMc00427
+3.4
16.3
peptide chain release factor RF-3 protein
compare
SMc02366
+3.3
25.5
transcriptional regulator
compare
SMc00129
+3.3
20.3
sensor histidine kinase
compare
SMc04385
+3.2
10.6
L-2-aminoadipate semialdehyde dehydrogenase (EC 1.2.1.31)
(from data)
compare
SMc01569
+3.2
9.4
carbamoyl phosphate synthase small subunit
compare
SMc02755
+3.2
18.0
S-adenosyl-L-homocysteine hydrolase
compare
SMc00321
+3.2
23.4
tRNA pseudouridine synthase B
compare
SMc04001
+3.2
5.2
phosphoribosylaminoimidazole carboxylase catalytic subunit protein
compare
SMc04454
+3.1
19.8
ABC transporter ATP-binding protein
compare
SMc02138
+3.1
7.1
acetylornithine transaminase
compare
SMc01801
+3.0
4.2
N-acetylglutamylphosphate reductase (EC 1.2.1.38)
(from data)
compare
SMc00320
+2.9
8.1
ribosome-binding factor A
compare
SMc02166
+2.8
20.3
dihydroorotase
compare
SMc02055
+2.8
6.0
phytoene synthase
compare
SMc00169
+2.7
22.6
malic enzyme
compare
SMc00482
+2.7
19.5
hypothetical protein
compare
SMc01439
+2.6
9.9
hypothetical protein
compare
SMc02245
+2.6
13.0
dihydroorotate dehydrogenase 2
compare
SMc00522
+2.6
16.7
ATP-dependent RNA helicase
compare
SMc04235
+2.6
12.5
tRNA-dihydrouridine synthase A
compare
SMc00710
+2.5
6.3
histidinol-phosphate aminotransferase
compare
SMc00761
+2.5
18.1
oxidoreductase
compare
SMc02757
+2.5
3.8
hypothetical protein
compare
SMc00017
+2.5
5.5
homoserine kinase
compare
SMc01907
+2.5
20.4
hypothetical protein
compare
SMc00998
+2.5
14.9
signal peptide protein
compare
SMc01222
+2.4
6.9
lipopolysaccharide core biosynthesis glycosyl transferase
compare
SMc03157
+2.4
15.8
outer membrane lipoprotein transmembrane
compare
SMc02058
+2.4
4.7
YAJC protein
compare
SMc00403
+2.4
13.7
NOL1/NOP2/SUN family signature protein
compare
SMc02095
+2.4
11.6
hypothetical protein
compare
SMc02504
+2.4
7.8
transcriptional regulator
compare
SMc01223
+2.4
4.0
transcriptional regulator
compare
SMc03111
+2.4
18.1
mannose 6-phosphate isomerase (EC 5.3.1.8)
(from data)
compare
SMc01858
+2.4
3.1
S-adenosyl-methyltransferase MraW
compare
SMc02309
+2.3
11.7
arsenate reductase
compare
SMc00070
+2.3
12.3
signal peptide protein
compare
SMc01726
+2.3
13.3
acetylglutamate kinase
compare
SMc04000
+2.3
2.7
hypothetical protein
compare
SMc00622
+2.2
14.9
ribonuclease D protein
compare
SM
_b21397
+2.2
1.4
hypothetical protein
compare
SMc03933
+2.2
17.9
sarcosine oxidase subunit B protein
compare
SMc00727
+2.2
15.7
3-hydroxybutyryl-CoA dehydrogenase
compare
SMc02766
+2.2
16.9
tryptophan synthase subunit beta
compare
SMc04383
+2.1
8.8
putative hydrolase, required for lysine catabolism
(from data)
compare
SMc03931
+2.1
17.0
sarcosine oxidase subunit alpha
compare
SMc01120
+2.1
8.9
hypothetical protein
compare
SM
_b20613
+2.1
12.3
C4-dicarboxylate transport transcriptional regulator
compare
SMc03930
+2.1
4.0
sarcosine oxidase subunit gamma
compare
SMc02246
+2.0
3.6
hypothetical protein
compare
SMc02765
+2.0
13.4
tryptophan synthase subunit alpha
compare
SMc00009
+2.0
8.5
cytochrome C oxidase subunit II protein
compare
SMc01024
+2.0
3.9
preprotein translocase subunit SecG
compare
SMc01613
+1.9
14.3
ribose-5-phosphate isomerase B
compare
SMc03158
+1.9
6.5
ABC transporter permease
compare
SM
_b20684
+1.9
14.7
hypothetical protein
compare
SMc00450
+1.9
6.2
protoheme IX farnesyltransferase
compare
SMc03159
+1.9
2.2
ABC transporter ATP-binding protein
compare
SMc03932
+1.8
8.5
sarcosine oxidase subunit delta
compare
SMc02273
+1.8
15.5
fatty acid synthase transmembrane protein
compare
SMc01768
+1.8
5.2
transcriptional regulator
compare
SMc01852
+1.8
10.7
pyrophosphate--fructose-6-phosphate 1-phosphotransferase
compare
SMc00648
+1.8
8.9
hypothetical protein
compare
SMc02272
+1.7
8.8
acyl-transferase transferase
compare
SMc03973
+1.7
7.4
hypothetical protein
compare
SM
_b20131
+1.7
1.4
oxidoreductase
compare
SMc00414
+1.7
9.6
hypothetical protein
compare
SMc04384
+1.6
3.9
(R)-2-hydroxyglutarate dehydrogenase (EC 1.1.99.39)
(from data)
compare
SMc01193
+1.6
10.1
hypothetical protein
compare
SM
_b20146
+1.6
5.6
pyridoxal phosphate biosynthetic protein
compare
SM
_b21192
+1.6
11.0
fructose-1,6-bisphosphate aldolase
compare
SM
_b21265
+1.6
5.6
glycosyltransferase
compare
SMc00325
+1.6
9.7
hypothetical protein
compare
SMc04002
+1.6
8.5
phosphoribosylaminoimidazole carboxylase ATPase subunit
compare
SMc02793
+1.5
8.4
hypothetical protein
compare
SMc02384
+1.5
7.9
glycosyltransferase transmembrane protein
compare
SMc01698
+1.5
9.5
oxidoreductase
compare
SMc02640
+1.5
6.0
UDP-glucuronic acid epimerase
compare
SM
_b21181
+1.5
7.8
glutaryl-CoA dehydrogenase
compare
SMc02497
+1.5
4.0
hypothetical protein
compare
SMc00904
+1.5
6.3
hypothetical protein
compare
SMc02056
+1.5
3.1
hypothetical protein
compare
SMc04441
+1.5
1.6
hypothetical protein
compare
SMc01885
+1.5
9.9
aminopeptidase P protein
compare
SMc00576
+1.4
2.2
hypothetical protein
compare
SMc00247
+1.4
10.2
phosphatidylcholine synthase
compare
SMc02520
+1.4
9.7
glycerol-3-phosphate dehydrogenase (EC 1.1.5.3)
(from data)
compare
SM
_b21170
+1.4
6.0
guanylate kinase
compare
SMc03028
+1.4
2.2
flagellar basal body rod protein FlgC
compare
SMc01131
+1.4
4.4
hypothetical protein
compare
SMc03037
+1.3
1.2
flagellin A
compare
SMc00026
+1.3
6.1
hypothetical protein
compare
SMc01153
+1.3
1.5
enoyl-CoA hydratase
compare
SMc02478
+1.3
8.0
hypothetical protein
compare
SMc01273
+1.3
9.8
S-formylglutathione hydrolase
compare
SMc01587
+1.3
1.9
hypothetical protein
compare
SMc00302
+1.3
4.6
hypothetical protein
compare
SMc04081
+1.3
7.1
glycosyl transferase (sulfolipid biosynthesis) protein
compare
SMa0887
+1.2
2.3
hypothetical protein
compare
SMc00787
+1.2
5.9
peptide ABC transporter permease
compare
SMc01800
+1.2
2.3
cytochrome C oxidase assembly transmembrane protein
compare
SMc01006
+1.2
5.3
hypothetical protein
compare
SMc03880
+1.2
7.1
hypothetical protein
compare
SMc00064
+1.2
5.0
hypothetical protein
compare
SMc02269
+1.2
5.1
capsular polysaccharide biosynthesis/export protein
compare
SMc02136
+1.2
5.4
Hsp33-like chaperonin
compare
SMc03961
+1.2
6.8
sulfolipid (UDP-sulfoquinovose) biosynthesis protein
compare
SMc04882
+1.2
2.9
transcriptional regulator
compare
SM
_b20994
+1.2
3.4
hypothetical protein
compare
SM
_b21372
+1.2
8.5
LacI family transcriptional regulator
compare
SMc01256
+1.2
6.5
L-serine dehydratase
compare
SMc00709
+1.2
3.9
hypothetical protein
compare
SMc00061
+1.2
5.4
hypothetical protein
compare
SMc02270
+1.1
9.3
capsular polysaccharide biosynthesis\export transmembrane protein
compare
SMc00329
+1.1
3.3
iron response regulator protein
compare
SMc03839
+1.1
1.5
hypothetical protein
compare
SM
_b21374
+1.1
4.5
tagatose kinase (EC 2.7.1.101)
(from data)
compare
SMc00973
+1.1
2.8
hypothetical protein
compare
SMc00826
+1.1
3.5
16S ribosomal RNA methyltransferase RsmE
compare
SMc04085
+1.1
2.9
hypothetical protein
compare
SMc02181
+1.1
8.8
bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase
compare
SMc00010
+1.1
3.5
cytochrome C oxidase polypeptide I transmembrane protein
compare
SMc02981
+1.1
1.5
amino acid efflux transmembrane protein
compare
SM
_b20026
+1.1
2.2
hypothetical protein
compare
SMc02434
+1.1
1.8
hypothetical protein
compare
SM
_b20751
+1.1
1.7
3-hydroxyisobutyrate dehydrogenase
compare
SMc01139
+1.1
5.5
RNA polymerase factor sigma-54
compare
SM
_b20815
+1.0
1.6
outer membrane protein assembly protein
compare
SMc03792
+1.0
1.3
hypothetical protein
compare
SMa2029
+1.0
1.3
amidohydrolase
compare
SM
_b21252
+1.0
8.7
glycosyltransferase
compare
SM
_b21256
+1.0
7.3
nucleotide sugar oxidase
compare
SMc02271
+1.0
5.1
ribitol type dehydrogenase
compare
SM
_b21253
+1.0
6.2
hypothetical protein
compare
SMc00352
+1.0
1.9
acetyltransferase
compare
SM
_b21264
+1.0
7.5
hypothetical protein
compare
SMc02575
+1.0
5.5
ATP-dependent protease peptidase subunit
compare
SMa5000
+1.0
1.4
hypothetical protein
compare
SMc02983
+1.0
5.4
ornithine, DAP, or arginine decarboxylase
compare
SM
_b20757
+1.0
6.2
methylmalonyl-CoA mutase
compare
SMc01765
+1.0
1.9
hypothetical protein
compare
SMc01847
+1.0
3.5
methyltransferase
compare
SMc01939
+1.0
1.0
urease subunit beta
compare
SMa0854
+1.0
0.7
3-ketoacyl-ACP reductase
compare
SMc00877
+1.0
6.3
transcriptional regulator
compare
SMc00789
+1.0
6.3
peptide ABC transporter ATP-binding protein
compare
SM
_b21491
+1.0
5.4
hypothetical protein
compare
SM
_b21208
+1.0
1.8
TetR family transcriptional regulator
compare
SMc01108
+1.0
5.1
tRNA (guanine-N(7)-)-methyltransferase
compare
SMa1630
+1.0
1.4
hypothetical protein
compare
SMc02684
+0.9
5.8
hydrolase/peptidase
compare
SMc00075
+0.9
3.7
ribosomal-protein-alanine acetyltransferase
compare
SMc03153
+0.9
4.4
keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase
compare
SMc02383
+0.9
6.2
hypothetical protein
compare
SMa1170
+0.9
1.8
hypothetical protein
compare
SMc02577
+0.9
5.6
ATP-dependent protease ATP-binding subunit HslU
compare
SM
_b20819
+0.9
1.5
ferredoxin
compare
SMa2289
+0.9
1.5
hypothetical protein
compare
SMc01834
+0.9
1.5
hypothetical protein
compare
SMc00171
+0.9
5.6
hypothetical protein
compare
SMc03963
+0.9
5.1
sulfolipid biosynthesis protein
compare
SMc01987
+0.9
7.1
dehydrogenase
compare
SMc03112
+0.9
7.2
B12-dependent methionine synthase
compare
SMc00539
+0.9
5.5
hypothetical protein
compare
SMc02051
+0.9
1.2
hypothetical protein
compare
SM
_b21266
+0.9
5.0
hypothetical protein
compare
Specific Phenotypes
For 8 genes in this experiment