Experiment set14IT085 for Pseudomonas fluorescens FW300-N2E2

Compare to:

10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine dihydrochloride

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_971 +2.0 1.9 hypothetical protein compare
Pf6N2E2_5677 +2.0 1.1 Carbon storage regulator compare
Pf6N2E2_5346 +1.7 2.3 rRNA small subunit methyltransferase I compare
Pf6N2E2_5936 +1.6 1.6 Low molecular weight protein tyrosine phosphatase (EC 3.1.3.48) compare
Pf6N2E2_6164 +1.5 1.1 tRNA-Val-TAC compare
Pf6N2E2_2597 +1.5 11.8 Flagellar regulatory protein FleQ compare
Pf6N2E2_2536 +1.5 1.5 Fumarate hydratase class II (EC 4.2.1.2) compare
Pf6N2E2_2508 +1.4 5.6 hypothetical protein compare
Pf6N2E2_204 +1.4 1.6 hypothetical protein compare
Pf6N2E2_2580 +1.4 5.3 Flagellar biosynthesis protein FliP compare
Pf6N2E2_659 +1.4 2.0 Transcriptional regulatory protein ompR compare
Pf6N2E2_2578 +1.3 4.5 Flagellar biosynthesis protein FliR compare
Pf6N2E2_1336 +1.3 2.6 AraC family transcription regulator compare
Pf6N2E2_613 +1.3 1.4 lipoprotein, putative compare
Pf6N2E2_2979 +1.3 5.9 Histone acetyltransferase HPA2 and related acetyltransferases conserved
Pf6N2E2_349 +1.2 2.8 hypothetical protein compare
Pf6N2E2_434 +1.2 2.8 hypothetical protein compare
Pf6N2E2_649 +1.2 2.5 General secretion pathway protein L compare
Pf6N2E2_2152 +1.2 2.1 hypothetical protein compare
Pf6N2E2_1162 +1.2 1.2 Transmembrane amino acid efflux protein compare
Pf6N2E2_2591 +1.2 6.1 Flagellar assembly protein FliH compare
Pf6N2E2_5452 +1.2 2.5 hypothetical protein compare
Pf6N2E2_2593 +1.2 7.3 Flagellar M-ring protein FliF compare
Pf6N2E2_4969 +1.1 2.1 hypothetical protein compare
Pf6N2E2_2592 +1.1 6.1 Flagellar motor switch protein FliG compare
Pf6N2E2_2583 +1.1 6.7 Flagellar motor switch protein FliM compare
Pf6N2E2_2919 +1.1 1.6 FIG00955830: hypothetical protein compare
Pf6N2E2_5695 +1.1 6.5 Flagellar basal-body rod protein FlgC compare
Pf6N2E2_2628 +1.1 5.0 Flagellar basal-body rod protein FlgF compare
Pf6N2E2_1169 +1.1 1.6 TnpR protein compare
Pf6N2E2_88 +1.1 1.4 hypothetical protein compare
Pf6N2E2_180 +1.1 1.9 Transcriptional regulator, MerR family compare
Pf6N2E2_2590 +1.1 7.8 Flagellum-specific ATP synthase FliI compare
Pf6N2E2_924 +1.0 1.4 Transcriptional regulator XdhR, TetR family compare
Pf6N2E2_2467 +1.0 2.8 5'-nucleotidase (EC 3.1.3.5) compare
Pf6N2E2_2577 +1.0 7.7 Flagellar biosynthesis protein FlhB compare
Pf6N2E2_919 +1.0 1.2 FIG00954373: hypothetical protein compare
Pf6N2E2_5462 +1.0 2.8 Signal transduction histidine kinase compare
Pf6N2E2_5694 +1.0 4.7 Flagellar basal-body rod protein FlgB compare
Pf6N2E2_2576 +1.0 7.7 Flagellar biosynthesis protein FlhA compare
Pf6N2E2_2406 +1.0 5.8 Periplasmic binding protein compare
Pf6N2E2_1978 +1.0 2.6 DNA topoisomerase III (EC 5.99.1.2) in PFGI-1-like cluster compare
Pf6N2E2_546 +1.0 1.2 FIG00966904: hypothetical protein compare
Pf6N2E2_4810 +0.9 5.8 Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24) compare
Pf6N2E2_5496 +0.9 3.1 FIG00953991: hypothetical protein compare
Pf6N2E2_3713 +0.9 1.9 Acyl carrier protein compare
Pf6N2E2_1476 +0.9 2.5 (AF179595) Vco33 compare
Pf6N2E2_3268 +0.9 6.1 Ribosome hibernation protein YhbH compare
Pf6N2E2_5841 +0.9 0.7 GlcG protein compare
Pf6N2E2_2594 +0.9 6.5 Flagellar hook-basal body complex protein FliE compare
Pf6N2E2_5697 +0.9 7.5 Flagellar hook protein FlgE compare
Pf6N2E2_2573 +0.9 5.8 RNA polymerase sigma factor for flagellar operon compare
Pf6N2E2_2507 +0.9 4.4 Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-) compare
Pf6N2E2_1141 +0.9 1.3 Lipid-A-disaccharide synthase (EC 2.4.1.182) compare
Pf6N2E2_5150 +0.9 4.9 Glucose-6-phosphate isomerase (EC 5.3.1.9) compare
Pf6N2E2_2602 +0.9 6.4 Flagellin protein FlaA compare
Pf6N2E2_952 +0.9 2.3 putative exported protein compare
Pf6N2E2_3074 +0.9 0.6 FIG00960973: hypothetical protein compare
Pf6N2E2_2585 +0.9 6.4 Flagellar hook-length control protein FliK compare
Pf6N2E2_2247 +0.9 2.0 Transcriptional regulator, AraC family compare
Pf6N2E2_2582 +0.9 3.2 Flagellar motor switch protein FliN compare
Pf6N2E2_1157 +0.9 2.0 hypothetical protein possibly connected to lactam utilization and allophanate hydrolase compare
Pf6N2E2_3466 +0.9 1.6 FIG000605: protein co-occurring with transport systems (COG1739) compare
Pf6N2E2_1656 +0.8 0.9 hypothetical protein compare
Pf6N2E2_3770 +0.8 0.7 ATP-dependent hsl protease ATP-binding subunit HslU compare
Pf6N2E2_2579 +0.8 4.5 Flagellar biosynthesis protein FliQ compare
Pf6N2E2_3326 +0.8 1.1 hypothetical protein compare
Pf6N2E2_2626 +0.8 5.9 Flagellar L-ring protein FlgH compare
Pf6N2E2_4797 +0.8 0.5 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3) compare
Pf6N2E2_5774 +0.8 3.0 FIG00954549: hypothetical protein compare
Pf6N2E2_2196 +0.8 2.6 FIG00953347: hypothetical protein compare
Pf6N2E2_1718 +0.8 1.3 Anthranilate synthase, amidotransferase component (EC 4.1.3.27) compare
Pf6N2E2_443 +0.8 2.0 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_234 +0.8 1.4 FIG00454312: hypothetical protein compare
Pf6N2E2_2563 +0.8 6.7 FIG00953562: hypothetical protein compare
Pf6N2E2_3096 +0.8 0.7 Periplasmic protein TonB, links inner and outer membranes compare
Pf6N2E2_99 +0.8 2.5 hypothetical protein compare
Pf6N2E2_2063 +0.8 1.5 Predicted cobalt transporter CbtA compare
Pf6N2E2_2503 +0.8 0.8 Glycosyl transferase, group 1 family protein compare
Pf6N2E2_4162 +0.8 1.0 DNA polymerase I (EC 2.7.7.7) compare
Pf6N2E2_4928 +0.8 0.7 Permeases of the major facilitator superfamily compare
Pf6N2E2_2438 +0.8 1.1 hypothetical protein compare
Pf6N2E2_1839 +0.8 1.4 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (EC 1.1.1.-) compare
Pf6N2E2_1720 +0.8 1.5 hypothetical protein compare
Pf6N2E2_4394 +0.8 1.5 Putrescine utilization regulator compare
Pf6N2E2_5229 +0.8 3.8 Transcriptional regulator, MerR family, associated with photolyase conserved
Pf6N2E2_3138 +0.8 1.5 YaeQ protein compare
Pf6N2E2_1339 +0.8 1.6 Acetylornithine deacetylase (EC 3.5.1.16) compare
Pf6N2E2_5898 +0.7 2.3 Integral membrane protein compare
Pf6N2E2_3493 +0.7 2.9 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) compare
Pf6N2E2_2175 +0.7 3.5 Transcriptional regulator, RpiR family compare
Pf6N2E2_3624 +0.7 4.7 Flagellar motor rotation protein MotB compare
Pf6N2E2_2627 +0.7 4.2 Flagellar basal-body rod protein FlgG compare
Pf6N2E2_1156 +0.7 1.3 hypothetical protein compare
Pf6N2E2_672 +0.7 1.1 ABC-type polar amino acid transport system, ATPase component compare
Pf6N2E2_2178 +0.7 3.8 FIG00960144: hypothetical protein compare
Pf6N2E2_2518 +0.7 0.8 Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) compare
Pf6N2E2_737 +0.7 1.1 Ribonucleotide reductase of class III (anaerobic), activating protein (EC 1.97.1.4) compare
Pf6N2E2_4479 +0.7 1.1 FIG001590: Putative conserved exported protein precursor compare
Pf6N2E2_4435 +0.7 4.7 Regulator of sigma D compare
Pf6N2E2_4982 +0.7 1.9 UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase (EC 6.3.2.-) compare
Pf6N2E2_4152 +0.7 2.2 alginate biosynthesis transcriptional regulatory protein AlgB compare
Pf6N2E2_6087 +0.7 1.8 Short-chain dehydrogenase/reductase SDR compare
Pf6N2E2_1778 +0.7 1.3 Positive regulator of CheA protein activity (CheW) compare
Pf6N2E2_2922 +0.7 2.5 hypothetical protein compare
Pf6N2E2_4943 +0.7 1.7 PspA/IM30 family protein compare
Pf6N2E2_4415 +0.7 1.2 Putative FMN hydrolase (EC 3.1.3.-); 5-Amino-6-(5'-phosphoribitylamino)uracil phosphatase compare
Pf6N2E2_956 +0.7 2.2 FIG01210404: hypothetical protein compare
Pf6N2E2_2623 +0.7 5.7 Flagellar hook-associated protein FlgK compare
Pf6N2E2_3761 +0.7 5.0 Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) compare
Pf6N2E2_1743 +0.7 1.4 Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1) compare
Pf6N2E2_3443 +0.7 2.6 Sulfate permease compare
Pf6N2E2_2121 +0.7 1.2 hypothetical protein compare
Pf6N2E2_1028 +0.7 1.6 Transcriptional regulator, LysR family compare
Pf6N2E2_2589 +0.7 2.5 Flagellar protein FliJ compare
Pf6N2E2_1044 +0.7 1.2 hypothetical protein compare
Pf6N2E2_476 +0.7 1.5 Permease of the drug/metabolite transporter (DMT) superfamily compare
Pf6N2E2_6067 +0.7 2.2 ABC transporter in pyoverdin gene cluster, ATP-binding component compare
Pf6N2E2_103 +0.7 2.4 Various polyols ABC transporter, ATP-binding component compare
Pf6N2E2_4362 +0.7 0.9 DNA-directed RNA polymerase omega subunit (EC 2.7.7.6) compare
Pf6N2E2_86 +0.7 2.3 FIG022160: hypothetical toxin compare
Pf6N2E2_1335 +0.7 1.8 Phospholipid-binding protein compare
Pf6N2E2_813 +0.7 1.3 Response regulator compare
Pf6N2E2_4043 +0.6 2.2 Outer membrane receptor proteins, mostly Fe transport compare
Pf6N2E2_2459 +0.6 1.1 Organic hydroperoxide resistance protein compare
Pf6N2E2_5739 +0.6 1.2 Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family compare
Pf6N2E2_1215 +0.6 1.3 probable membrane protein YPO2362 compare
Pf6N2E2_309 +0.6 1.0 FIG00957702: hypothetical protein compare
Pf6N2E2_2352 +0.6 1.8 Type III secretion bridge between inner and outermembrane lipoprotein (YscJ,HrcJ,EscJ, PscJ) compare
Pf6N2E2_358 +0.6 1.6 Haloacid dehalogenase, type II compare
Pf6N2E2_3944 +0.6 1.8 Protein of unknown function DUF81 compare
Pf6N2E2_1027 +0.6 1.1 Cysteine dioxygenase (EC 1.13.11.20) compare
Pf6N2E2_159 +0.6 1.9 DNA damage resistance protein (from data) compare
Pf6N2E2_4562 +0.6 1.8 L-cystine uptake protein TcyP compare
Pf6N2E2_1155 +0.6 2.0 Cyn operon transcriptional activator compare
Pf6N2E2_2724 +0.6 1.8 Nitroreductase family protein compare
Pf6N2E2_3477 +0.6 0.4 2-Oxobutyrate oxidase, putative compare
Pf6N2E2_1032 +0.6 0.9 Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB compare
Pf6N2E2_5396 +0.6 4.1 YheO-like PAS domain compare
Pf6N2E2_6071 +0.6 1.8 FIG139991: Putative thiamine pyrophosphate-requiring enzyme compare
Pf6N2E2_238 +0.6 0.8 hypothetical protein compare
Pf6N2E2_2069 +0.6 1.3 FIG00953703: hypothetical protein compare
Pf6N2E2_2605 +0.6 5.9 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180) compare
Pf6N2E2_3625 +0.6 4.5 Flagellar motor rotation protein MotA compare
Pf6N2E2_2913 +0.6 3.5 Transcriptional regulator PobR, AraC family compare
Pf6N2E2_464 +0.6 1.3 Sensory histidine kinase QseC compare
Pf6N2E2_2397 +0.6 1.1 Two-component system response regulator QseB compare
Pf6N2E2_338 +0.6 0.7 Transcriptional regulator, AraC family compare
Pf6N2E2_2350 +0.6 1.7 Oxygen-regulated invasion protein OrgB compare
Pf6N2E2_2999 +0.6 1.0 probable tail fiber assembly protein compare
Pf6N2E2_1821 +0.6 0.9 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf6N2E2_1236 +0.6 1.2 Transcriptional regulator, LysR family compare
Pf6N2E2_4478 +0.6 3.0 FIG019278: hypothetical protein compare
Pf6N2E2_2790 +0.6 1.1 Helix-turn-helix motif compare
Pf6N2E2_5640 +0.6 1.1 FIG00955597: hypothetical protein compare
Pf6N2E2_2622 +0.6 4.4 Flagellar hook-associated protein FlgL compare
Pf6N2E2_318 +0.6 1.7 Gluconolactonase (EC 3.1.1.17) compare
Pf6N2E2_2624 +0.6 3.2 Flagellar protein FlgJ [peptidoglycan hydrolase] (EC 3.2.1.-) compare
Pf6N2E2_2452 +0.6 2.6 Spermidine synthase (EC 2.5.1.16) compare
Pf6N2E2_1082 +0.6 1.2 CopG protein compare
Pf6N2E2_3332 +0.6 2.3 L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1) compare
Pf6N2E2_1875 +0.6 1.0 Protoporphyrinogen oxidase (EC 1.3.3.4) compare
Pf6N2E2_1699 +0.6 1.3 FIG00955832: hypothetical protein compare
Pf6N2E2_698 +0.6 1.0 Heme d1 biosynthesis protein NirH compare
Pf6N2E2_2040 +0.6 2.7 D-beta-hydroxybutyrate permease compare
Pf6N2E2_6095 +0.6 1.7 tRNA-Asn-GTT compare
Pf6N2E2_4254 +0.6 2.2 HflK protein compare
Pf6N2E2_947 +0.6 2.0 Multidrug efflux membrane fusion protein MexE compare
Pf6N2E2_1171 +0.6 1.2 LysR family transcriptional regulator PA2877 compare
Pf6N2E2_6080 +0.6 1.7 Short-chain dehydrogenase, associated with 2-hydroxychromene-2-carboxylate isomerase family protein compare
Pf6N2E2_5696 +0.5 3.2 Flagellar basal-body rod modification protein FlgD compare
Pf6N2E2_1213 +0.5 1.4 hypothetical protein compare
Pf6N2E2_5671 +0.5 3.2 Succinylglutamate desuccinylase (EC 3.5.1.96) compare
Pf6N2E2_876 +0.5 1.9 Cobalt-containing nitrile hydratase subunit beta (EC 4.2.1.84) compare
Pf6N2E2_5714 +0.5 0.7 Flavoprotein WrbA compare
Pf6N2E2_1722 +0.5 1.9 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157) compare
Pf6N2E2_5013 +0.5 0.8 Putative membrane protein compare
Pf6N2E2_855 +0.5 0.8 Transcriptional regulator, IclR family compare
Pf6N2E2_69 +0.5 3.5 Probable type IV pilus assembly FimV-related transmembrane protein compare
Pf6N2E2_5164 +0.5 2.3 Periplasmic hemin-binding protein compare
Pf6N2E2_1294 +0.5 1.0 Carbon starvation protein A compare
Pf6N2E2_2933 +0.5 1.4 hypothetical protein compare
Pf6N2E2_5862 +0.5 1.1 Lipoprotein, putative compare
Pf6N2E2_3912 +0.5 2.1 Alpha-ketoglutarate-dependent taurine dioxygenase (EC 1.14.11.17) compare
Pf6N2E2_4543 +0.5 1.8 hypothetical protein compare
Pf6N2E2_2584 +0.5 2.9 Flagellar biosynthesis protein FliL compare
Pf6N2E2_3569 +0.5 0.9 hypothetical protein compare
Pf6N2E2_5534 +0.5 0.7 FIG000859: hypothetical protein YebC compare
Pf6N2E2_617 +0.5 1.4 Probable glutathione S-transferase compare
Pf6N2E2_3161 +0.5 1.5 hypothetical protein compare
Pf6N2E2_1905 +0.5 1.3 Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16) compare
Pf6N2E2_5135 +0.5 2.5 FIG00953333: hypothetical protein compare
Pf6N2E2_638 +0.5 1.0 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein compare
Pf6N2E2_1089 +0.5 1.0 Delta 1-piperideine-2-carboxylate reductase (EC 1.5.1.21) / Delta 1-pyrroline-2-carboxylate reductase (EC 1.5.1.1) compare
Pf6N2E2_1749 +0.5 1.3 Cyn operon transcriptional activator compare
Pf6N2E2_6081 +0.5 1.2 Transcriptional regulator, TetR family, in cluster with 2-hydroxychromene-2-carboxylate isomerase family protein, glutathione-dependent compare
Pf6N2E2_2599 +0.5 2.6 Flagellar biosynthesis protein FliS compare
Pf6N2E2_5625 +0.5 2.0 COG0613, Predicted metal-dependent phosphoesterases (PHP family) compare
Pf6N2E2_738 +0.5 2.3 Methyl-accepting chemotaxis protein I (serine chemoreceptor protein) compare
Pf6N2E2_27 +0.5 1.8 Mechanosensitive ion channel compare


Specific Phenotypes

For 11 genes in this experiment

For resistance P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine dihydrochloride in Pseudomonas fluorescens FW300-N2E2

For resistance P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine dihydrochloride across organisms