Protein Info for RS_RS06625 in Ralstonia solanacearum GMI1000

Annotation: UDP-4-amino-4-deoxy-L-arabinose-oxoglutarate aminotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 332 transmembrane" amino acids 230 to 252 (23 residues), see Phobius details amino acids 264 to 286 (23 residues), see Phobius details PF13641: Glyco_tranf_2_3" amino acids 4 to 140 (137 residues), 30.9 bits, see alignment E=2.9e-11 PF00535: Glycos_transf_2" amino acids 7 to 168 (162 residues), 100.7 bits, see alignment E=8.7e-33

Best Hits

Swiss-Prot: 53% identical to ARNC_ECO24: Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (arnC) from Escherichia coli O139:H28 (strain E24377A / ETEC)

KEGG orthology group: K10012, undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase [EC: 2.7.8.30] (inferred from 100% identity to rso:RSc1321)

MetaCyc: 53% identical to undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (Escherichia coli K-12 substr. MG1655)
RXN0-3521 [EC: 2.4.2.53]

Predicted SEED Role

"Polymyxin resistance protein ArnC, glycosyl transferase (EC 2.4.-.-)" in subsystem Lipid A-Ara4N pathway ( Polymyxin resistance ) (EC 2.4.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.-.-

Use Curated BLAST to search for 2.4.-.- or 2.4.2.53 or 2.7.8.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XZS4 at UniProt or InterPro

Protein Sequence (332 amino acids)

>RS_RS06625 UDP-4-amino-4-deoxy-L-arabinose-oxoglutarate aminotransferase (Ralstonia solanacearum GMI1000)
MKTPALSVVIPVYNEEEGLAALFARLYPALDALGISYEVVFINDGSRDRSAAMLAEQFRA
RPDTTRVVLFNGNYGQHMAILAGFEHARGERVITLDADLQNPPEEIGRLVAKLDEGYDYV
GTIRRNRQDTWFRRTASRAMNSLRERITHIKMTDQGCMLRAYSRPIVDTINRCREVNTFI
PALGYTFAKNPTEIEVDHEERFAGESKYSLYKLIRLNFDLVTGFSVVPLQWFSAIGMLLS
LASAVLFVLLVVRRFVLGAEVQGVFTLFAITFFLMGVLLFGIGLLGEYIGRIYQQVRERP
RYLVQGVLEDAPNLHEAGAADLNVSKAQRGAA