Protein Info for RR42_RS33755 in Cupriavidus basilensis FW507-4G11
Annotation: tartrate dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 71% identical to TTUC3_AGRVI: Probable tartrate dehydrogenase/decarboxylase TtuC' (ttuC') from Agrobacterium vitis
KEGG orthology group: K07246, tartrate dehydrogenase/decarboxylase / D-malate dehydrogenase [EC: 1.1.1.83 1.1.1.93 4.1.1.73] (inferred from 78% identity to psp:PSPPH_1578)MetaCyc: 51% identical to D-malate/3-isopropylmalate dehydrogenase (decarboxylating) (Escherichia coli K-12 substr. MG1655)
Tartrate dehydrogenase. [EC: 1.1.1.93, 4.1.1.73]; 3-isopropylmalate dehydrogenase. [EC: 1.1.1.93, 4.1.1.73, 1.1.1.85]; D-malate dehydrogenase (decarboxylating). [EC: 1.1.1.93, 4.1.1.73, 1.1.1.85, 1.1.1.83]
Predicted SEED Role
"Tartrate dehydrogenase (EC 1.1.1.93) @ Tartrate decarboxylase (EC 4.1.1.73) @ D-malic enzyme (EC 1.1.1.83)" (EC 1.1.1.83, EC 1.1.1.93, EC 4.1.1.73)
MetaCyc Pathways
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- L-leucine biosynthesis (6/6 steps found)
- gentisate degradation II (4/4 steps found)
- 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
- D-malate degradation (1/1 steps found)
- methylgallate degradation (4/6 steps found)
- protocatechuate degradation I (meta-cleavage pathway) (5/8 steps found)
- superpathway of vanillin and vanillate degradation (6/10 steps found)
- butanol and isobutanol biosynthesis (engineered) (4/8 steps found)
- syringate degradation (5/12 steps found)
- aliphatic glucosinolate biosynthesis, side chain elongation cycle (10/30 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Butanoate metabolism
- Glyoxylate and dicarboxylate metabolism
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.83, 1.1.1.85, 1.1.1.93, 4.1.1.73
Use Curated BLAST to search for 1.1.1.83 or 1.1.1.85 or 1.1.1.93 or 4.1.1.73
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0C4YUM5 at UniProt or InterPro
Protein Sequence (358 amino acids)
>RR42_RS33755 tartrate dehydrogenase (Cupriavidus basilensis FW507-4G11) MREYKIAAIPGDGIGPEVISAGLQVLETLAGQDGGFKLGVEHFPWSSDYYLEKGYYIPEG GLERLKQFDAIFFGAVGALNVPDHVSLWGLRLPIAQGFDQYANVRPARVLPGVKSPLANG KDIDWVIIRENSEGEYAGNGGRTHRGLPIETGTETAVFTRAGVERIHRFAFEIAQKRPRK HLTLVTKSNAQRFGMVMWDEIFYEVAKDYPDVKVDRELVDAVTTRMVLKPHTLDVVVATN LHADILSDLAAALSGSLGIAPTANLNPERLFPSMFEPIHGSAFDITGKGVANPIATFWTA AMMLEHLGEKPAAERLMAAIEQITANGVFTPDLGGHATTASVTEAICKVLADQVKKAA