Protein Info for RR42_RS14065 in Cupriavidus basilensis FW507-4G11

Annotation: serine peptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details TIGR02037: peptidase Do" amino acids 40 to 487 (448 residues), 517.1 bits, see alignment E=2e-159 PF00089: Trypsin" amino acids 105 to 268 (164 residues), 63.3 bits, see alignment E=9.2e-21 PF13365: Trypsin_2" amino acids 114 to 245 (132 residues), 132.5 bits, see alignment E=6.1e-42 PF13180: PDZ_2" amino acids 300 to 375 (76 residues), 53.4 bits, see alignment E=7.6e-18 PF00595: PDZ" amino acids 301 to 344 (44 residues), 36.2 bits, see alignment 2e-12 PF17820: PDZ_6" amino acids 311 to 364 (54 residues), 43.9 bits, see alignment 4.9e-15 amino acids 426 to 472 (47 residues), 31.3 bits, see alignment 4.1e-11

Best Hits

Swiss-Prot: 48% identical to DEGPL_PSEPW: Probable periplasmic serine endoprotease DegP-like (PputW619_1070) from Pseudomonas putida (strain W619)

KEGG orthology group: K01362, [EC: 3.4.21.-] (inferred from 86% identity to reu:Reut_A2256)

Predicted SEED Role

"HtrA protease/chaperone protein"

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0C4YDT8 at UniProt or InterPro

Protein Sequence (496 amino acids)

>RR42_RS14065 serine peptidase (Cupriavidus basilensis FW507-4G11)
MSPRSPALARTVLMAAILAFAPFVAELSHAQAAAPSYNLPDFTDLVEKAGPAVVNIRTTE
RVRARGTPGGAEDDEMAEFFRRFFGVPMPGAPTPPGAPRRGQPPQGEEQSRGVGSGFVIS
QDGYVMTNAHVVADAETIYVTLPDKREFKAKLIGSDKRTDVALLKVDATGLPKLTLGDSD
KVRAGEWVLAIGSPFGLDNTVTAGIVSAKGRDTGDYLPFIQTDVAVNPGNSGGPLINLRG
EVIGINSQIYSRSGGYMGISFAIPIDEAMRVTEQLKATGKVTRGRIAVAIGDVTKEVADS
LGLGRARGALVGSVEPGGPAEKAGIEAGDIILKFNGRDIEKASDLPRMVGDIKPGTRVPL
QLWRKGATRDVSITVAELDVDAKVKTRGNGSRDDSTPPAAKPNALGLIVNEIPDAKLKDL
KIKSGVEVEQSDGPAMRAGIRPGDIILRLGDSDVTSPKQFNELVKGLDRAKIAAVFVRRG
DATQVLTLRPGTMSSR