Protein Info for Psyr_3345 in Pseudomonas syringae pv. syringae B728a

Annotation: TonB-dependent siderophore receptor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 705 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details PF07715: Plug" amino acids 68 to 166 (99 residues), 69.5 bits, see alignment E=3.3e-23 TIGR01783: TonB-dependent siderophore receptor" amino acids 72 to 705 (634 residues), 361 bits, see alignment E=6.9e-112 PF00593: TonB_dep_Rec_b-barrel" amino acids 237 to 673 (437 residues), 236.7 bits, see alignment E=9.7e-74

Best Hits

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 100% identity to psb:Psyr_3345)

Predicted SEED Role

"Ferrichrome-iron receptor" in subsystem Iron acquisition in Vibrio or Transport of Iron

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZR45 at UniProt or InterPro

Protein Sequence (705 amino acids)

>Psyr_3345 TonB-dependent siderophore receptor (Pseudomonas syringae pv. syringae B728a)
MSTSFQCPRRPGSFLLSALSLAMFSGGAVHAAETATLPATQVSATATEAQEGYSAKSAST
ASKSDVPLKEEAQSVNVVTPQTIADYNVRSLDDAMKFVSGVSQGNTLGNTKDSLVKRGFG
TNDDGSVLRDGVRSVMSKNFGATTERVEVLKGPASLLYGAMEPGGVINVISKQPQYVQST
TLSGSAYSEGGGSFGIDTTGPLGDSGLAYRLVAERQSEDYWRNYGVDQHTLVAPSLSWTG
ERASLTLAYEYNDYVSPFDRGTVFTGGHPADISYDKRLDEKWANTVGISETATARFEYQL
SDDWKTRLTYGWNNDRYSLSIAQPSTLSSSGVLRRQANGGHYDYETRYASWDFIGKQEVF
GQQHDLLIGAEQEDSDKYRGKTYRNGVQGGFNIFSPSYGSLAEPSVVSTTTSNLSNQLTS
SSIYLKDNWHLDDRWILVLGGRYQHYDQHIAQGLGASRNTNLNKNDDIFLPMAGLVFKVN
EDLSLYGNYSRSFVPNEAVNDDGTTFDPEEGRSYEVGAKYALAPELDFNLAVFDIEKKNV
VTTVATGVSEAAGKVGSQGVELDVTGRIAERWDMIGTYAYTHTEVLDDPKNEGNRLSQAP
KHTASLYLTHHLQVPSGLGEWHAGGGARYVGDRAGDNANTFYMSSYTVADAFLRWDVPMA
GYKTRLQLNVDNLFDKQYYPSSTDSPLQVNVGEPRTARLSASVTF