Protein Info for Psyr_3273 in Pseudomonas syringae pv. syringae B728a

Annotation: 2-keto-myo-inositol dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 301 TIGR04379: myo-inosose-2 dehydratase" amino acids 10 to 296 (287 residues), 382.7 bits, see alignment E=6.7e-119 PF01261: AP_endonuc_2" amino acids 41 to 282 (242 residues), 88.4 bits, see alignment E=3.2e-29

Best Hits

Swiss-Prot: 49% identical to MOCC_RHIML: Rhizopine catabolism protein MocC (mocC) from Rhizobium meliloti

KEGG orthology group: K03335, inosose dehydratase [EC: 4.2.1.44] (inferred from 100% identity to psb:Psyr_3273)

MetaCyc: 37% identical to myo-inosose-2 dehydratase (Klebsiella aerogenes)
Myo-inosose-2 dehydratase. [EC: 4.2.1.44]

Predicted SEED Role

"Inosose dehydratase (EC 4.2.1.44)" in subsystem Inositol catabolism (EC 4.2.1.44)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.44

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZRB7 at UniProt or InterPro

Protein Sequence (301 amino acids)

>Psyr_3273 2-keto-myo-inositol dehydratase (Pseudomonas syringae pv. syringae B728a)
MPVSTSTPRIRIGINPISWSNDDLPALGGETPLSTALSEGKAIGYEGFELNGKFPKDAKG
VGDVLRPHDLALVSGWYSSRLARRSVAEEIEAITPHVQLLAENGASVLVYGEVADSIQGQ
RIPLVERPRFHTDAAWHEYAEKLTELARFTLSRGVRLAYHHHMGAYVESPQDIDRLMALT
GEEVGLLFDSGHCYMGGGEPLQVLSKHIQRICHVHFKDVRKPVVQLARNNLWSFPDCIIN
GTFTVPGDGDIDFSALLKVLLQADYRGWLVVEAEQDPAVAPSYVYAKKGYDTLRQLLADA
R