Protein Info for Psyr_2745 in Pseudomonas syringae pv. syringae B728a

Annotation: Pyridoxal-5'-phosphate-dependent enzyme, beta subunit:Ornithine cyclodeaminase/mu-crystallin

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03945: 2,3-diaminopropionate biosynthesis protein SbnA
  • amino acids 8 to 304 (297 residues), 407.1 bits, E=4.2e-126
• PF00291: PALP
  • amino acids 19 to 288 (270 residues), 205.9 bits, E=9.2e-65
• TIGR03944: 2,3-diaminopropionate biosynthesis protein SbnB
  • amino acids 357 to 678 (322 residues), 417.8 bits, E=2.7e-129
• PF02423: OCD_Mu_crystall
  • amino acids 375 to 668 (294 residues), 103.3 bits, E=1.3e-33

Best Hits

KEGG orthology group: K01738, cysteine synthase A [EC: 2.5.1.47] (inferred from 100% identity to psb:Psyr_2745)

Predicted SEED Role

"Ornithine cyclodeaminase (EC 4.3.1.12)" in subsystem Arginine and Ornithine Degradation (EC 4.3.1.12)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• L-arginine degradation VII (arginase 3 pathway) (2/2 steps found)
• L-cysteine biosynthesis I (2/2 steps found)
• L-ornithine degradation I (L-proline biosynthesis) (1/1 steps found)
• L-proline biosynthesis II (from arginine) (1/2 steps found)
• seleno-amino acid biosynthesis (plants) (3/5 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Cysteine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.47

Use Curated BLAST to search for 2.5.1.47 or 4.3.1.12

Sequence Analysis Tools

See Q4ZST8 at UniProt or InterPro

Protein Sequence (685 amino acids)

>Psyr_2745 Pyridoxal-5'-phosphate-dependent enzyme, beta subunit:Ornithine cyclodeaminase/mu-crystallin (Pseudomonas syringae pv. syringae B728a)
MYNSLCEIVHDQAFLKVTGLGADFFLKMESLNPAGSIKLKTAVGLINDLQARGLLGPDTI
LIESSSGNLGVALAMICAERGIPFTCVVDPNSSSHNIRMMRSYGAEVIQVEIPDANGGFL
GTRIELIRQKVASDPRYVWLNQYENAANPRAHARTTARSISQHFGHVDYVFVGAGTTGTL
MGCIQHFQRHHPTTRIIAVDSVGSVTFDTPASRRFIPGLGTSQRPPIFNADGIHALEMVP
ESHTVAMCRILARSKGLLVGGSTATVIAAVHAWRERIEPGSVVVALSPDWGERYLDTLYD
DHWVEQRFGREVLSMTLADLSSPVAETSPESMEAPALSRHSSWTVWLAAERMKRAAFHVL
DGEVVARLLAADPLACIDDVQAAYLDHEAGRTINPDSYFLRFAQAPANRIIALPASLSGE
QPVSGIKWISSFPGNIDTGLQRASAVLILNDPLTGYAFACLEASRISAMRTAASAVLAAR
WMSRQQRHVGRMSFIGAGFIARTILDMFVSDGWTMDAVSVFDQHQDSALALVGHASRHGL
HSEPSDLATCLQADVVVFATTAPSPYVLEPVFQPGQRVLNISLRDLGPEVIAQANNILDD
VEHCLKAQTSPDLAVQQYQHRSFITGTLAQLMTGQVELSPDKASIFSPFGLGVLDLAVGQ
RVYRQAVAEGSALPVPQFFFESARW