Protein Info for Pf6N2E2_5600 in Pseudomonas fluorescens FW300-N2E2

Annotation: Methionyl-tRNA synthetase (EC 6.1.1.10)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 657 PF09334: tRNA-synt_1g" amino acids 1 to 371 (371 residues), 480.7 bits, see alignment E=6.2e-148 TIGR00398: methionine--tRNA ligase" amino acids 1 to 511 (511 residues), 541.5 bits, see alignment E=2.2e-166 PF19303: Anticodon_3" amino acids 398 to 493 (96 residues), 26.9 bits, see alignment E=7.8e-10 TIGR00399: methionine--tRNA ligase, beta subunit" amino acids 533 to 657 (125 residues), 140.5 bits, see alignment E=3.1e-45 PF01588: tRNA_bind" amino acids 561 to 655 (95 residues), 87.5 bits, see alignment E=1e-28

Best Hits

Swiss-Prot: 95% identical to SYM_PSEF5: Methionine--tRNA ligase (metG) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K01874, methionyl-tRNA synthetase [EC: 6.1.1.10] (inferred from 100% identity to pba:PSEBR_a4380)

MetaCyc: 65% identical to methionine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Methionine--tRNA ligase. [EC: 6.1.1.10]; 6.1.1.10 [EC: 6.1.1.10]; RXN-23924 [EC: 6.1.1.10]

Predicted SEED Role

"Methionyl-tRNA synthetase (EC 6.1.1.10)" (EC 6.1.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A284 at UniProt or InterPro

Protein Sequence (657 amino acids)

>Pf6N2E2_5600 Methionyl-tRNA synthetase (EC 6.1.1.10) (Pseudomonas fluorescens FW300-N2E2)
MLEYIQTDMWVRFQKHRGNQCIYVCADDAHGSAIMLRAEKEGITPEQLIANVQAEHSADF
AEFLVDFDNFHSTHAEENRELSSQIYLKLRDAGHIATRSITQYFDPEKKMFLADRFIKGT
CPKCGTEDQYGDNCEKCGATYAPTDLKDPKSAISGATPVLKDSQHFFFKLPDFQQMLQAW
TRSGTLQDAVANKIAEWLDAGLQQWDISRDAPYFGFEIPDEPGKYFYVWLDAPIGYMASF
KNLCNRTPELDFDAFWGKDSTAELYHFIGKDIVNFHALFWPAMLEGAGFRKPTGINVHGY
LTVNGQKMSKSRGTFIKARTYLDHLSPEYLRYYYAAKLGRGVDDLDLNLEDFVQKVNSDL
VGKVVNIASRCAGFIHKGNAGVLVAGNAAPELTDAFLAAAPSIADAYESRDFARAMREIM
ALADRANAWIADKAPWSLAKQEGKQDEVQAICALGINLFRQLVIFLKPVLPLLAADAEAF
LNVAPLTWNDHQTLLSNHQLNAFKPLMTRIDATKVQAMTDASKEDLAASQTDTGAPAGNG
ELAKDPLSPEIDFDTFAAVDLRVALIVKAEAVEGADKLLRLTLDIGDEQRNVFSGIKSAY
PDPAKLEGRLTMMIANLKPRKMRFGISEGMVMAAGPGGEEIYLLSPDSGAKPGQRIK