Protein Info for Pf6N2E2_3798 in Pseudomonas fluorescens FW300-N2E2

Updated annotation (from data): Imidazolonepropionase (EC 3.5.2.7)
Rationale: Specifically important for utilizing L-Histidine. Automated validation from mutant phenotype: the predicted function (IMIDAZOLONEPROPIONASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: Imidazolonepropionase (EC 3.5.2.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01224: imidazolonepropionase
  • amino acids 21 to 396 (376 residues), 492.2 bits, E=4.4e-152
• PF01979: Amidohydro_1
  • amino acids 57 to 383 (327 residues), 77.3 bits, E=1.4e-25
• PF07969: Amidohydro_3
  • amino acids 105 to 375 (271 residues), 61.2 bits, E=1.4e-20

Best Hits

Swiss-Prot: 90% identical to HUTI_PSEPF: Imidazolonepropionase (hutI) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01468, imidazolonepropionase [EC: 3.5.2.7] (inferred from 98% identity to pba:PSEBR_a385)

MetaCyc: 83% identical to imidazolonepropionase (Pseudomonas fluorescens)
Imidazolonepropionase. [EC: 3.5.2.7]

Predicted SEED Role

"Imidazolonepropionase (EC 3.5.2.7)" in subsystem Histidine Degradation (EC 3.5.2.7)

MetaCyc Pathways

• L-histidine degradation II (5/5 steps found)
• L-histidine degradation VI (7/8 steps found)
• L-histidine degradation I (4/4 steps found)
• L-histidine degradation III (4/6 steps found)

KEGG Metabolic Maps

• Histidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.2.7

Sequence Analysis Tools

See A0A160A1E6 at UniProt or InterPro

Protein Sequence (400 amino acids)

>Pf6N2E2_3798 Imidazolonepropionase (EC 3.5.2.7) (Pseudomonas fluorescens FW300-N2E2)
MKTLWQNCHAATMAQGVYSIIEDAAIVTQGKHIQWIGPRAELPTGDYPAVNDLKGAWVTP
GLIDCHTHTVFGGNRSGEFEQRLQGVSYAEIAAAGGGIASTVRATRAASEDELFASAAKR
LGSLMRDGVTTVEIKSGYGLDLASERKILRVIRRLGAELPVSVRSTCLAAHALPPEYKDR
ADDYIEHICSEMLPALAAEGLVDAVDAFCEYLAFSPAQVERVFITAQQLGLPVKLHAEQL
SSLHGSSLAARYQALSADHLEFMTEDDAKAMAASGTVAVLLPGAFYFLRETQLPPMEALR
KHGVKIAVASDLNPGTSPALSLRLMLNMACTCFRMTPEEALAGVTIHAAQALGMAQTHGS
LEAGKVADFVAWHIDRPADLAYWLGGDLEKRVVRHGVEID