Protein Info for MPMX19_05312 in Azospirillum sp. SherDot2
Annotation: 4,4'-diapophytoene desaturase (4,4'-diapolycopene-forming)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 42% identical to CRTN_METSP: 4,4'-diapophytoene desaturase (4,4'-diapolycopene-forming) (crtN) from Methylomonas sp.
KEGG orthology group: K10027, phytoene dehydrogenase [EC: 1.14.99.-] (inferred from 60% identity to aca:ACP_2303)MetaCyc: 42% identical to diapophytoene desaturase (Methylomonas sp. 16a)
RXN-12224 [EC: 1.3.8.2]
Predicted SEED Role
"Phytoene desaturase, neurosporene or lycopene producing (EC 1.3.-.-)" in subsystem Carotenoids (EC 1.3.-.-)
MetaCyc Pathways
- 4,4'-diapolycopenedioate biosynthesis (1/9 steps found)
- staphyloxanthin biosynthesis (1/9 steps found)
KEGG Metabolic Maps
- 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) degradation
- Benzoate degradation via hydroxylation
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Carotenoid biosynthesis - General
- Limonene and pinene degradation
- Naphthalene and anthracene degradation
- Nicotinate and nicotinamide metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.14.99.-, 1.3.-.-
Use Curated BLAST to search for 1.14.99.- or 1.3.-.- or 1.3.8.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (499 amino acids)
>MPMX19_05312 4,4'-diapophytoene desaturase (4,4'-diapolycopene-forming) (Azospirillum sp. SherDot2) MLLALTGAKVTVLERQDRIGGRSAGFSLDGYRFDSGPTFFLYPRILEEIFEACGRRLSDD VDLIRLDPLYRLVFESGGELRVHAQMKALMAEIAKLSPGDADGLPRFLADNRAKMERFRP VLESDFSSPWALASLPMLKALGRLAPHRSVDRDLSRYFQDPRIRLAFSFQSKYLGMSPFR CPSLFTILSFLEHEHGIFHPRGGTESVMEAMRRVAESLGVTVRLNETVRHIKFQGRRAVG VRTDAGDYPADALVINADFARAMTTLVPDDLRRRWSDARIATKKFSCSTFMMYLGIEDKV DGLDHHTVYLAEDYARNLAEIEECRELPRRPSIYVQNACVTDPDLAPPGGSTLYILVPVG HQRGHIDWKAEAPHYRRLVLDRLAGFGLTDLERRIRVERVVTPADWDSQFAVHRGATFNL AHSLDQMLHRRPRNRFEDLDGVYLVGGGTHPGSGLPVIFEGARITARLLAQELGLPTPWR TKTMGLSGMLRQAVAGGMA