Protein Info for MPMX19_04116 in Azospirillum sp. SherDot2

Annotation: Protein-glutamate methylesterase/protein-glutamine glutaminase of group 3 operon

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 391 transmembrane" amino acids 206 to 218 (13 residues), see Phobius details amino acids 241 to 256 (16 residues), see Phobius details PF00072: Response_reg" amino acids 19 to 123 (105 residues), 73.1 bits, see alignment E=2e-24 PF01339: CheB_methylest" amino acids 206 to 380 (175 residues), 181.4 bits, see alignment E=1.4e-57

Best Hits

Predicted SEED Role

"Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61)" in subsystem Bacterial Chemotaxis (EC 3.1.1.61)

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.61

Use Curated BLAST to search for 3.1.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (391 amino acids)

>MPMX19_04116 Protein-glutamate methylesterase/protein-glutamine glutaminase of group 3 operon (Azospirillum sp. SherDot2)
MSGQTNGGPLTTGTRRIRVLVVEDSPVIQQLLSHVIGADPRLEVAGIASSGEQALRMIER
TAPDVVSLDIRLPGIDGFEVTSRIMRQTPLPIVVVASDVRDLDIPMRALQAGALAVVEKP
GSMARADYQTVAHHLCTQLVIMSQVKVIRHRITARAARPASGMSAGASADAPSQDNRLAD
SRLPDNRLPDEAVAPDGRLRRFRALGLVASTGGPAALAKILRDLPADFPMPVFVVQHMGA
PFMAGFASWLGTVSALPVRLGEAGLPPRPGTVYVAPGDRHLLVDGATLRLTADPMVCGQR
PSGEVLFQSMARAYGASGIGVLLTGMGEDGARGLVDMRTAGAYTIAEHASTAVIHSMPGT
AVRLGGAVEELPLDRVAGRLLQLTSDDKMAS