Protein Info for MPMX19_01517 in Azospirillum sp. SherDot2

Annotation: O-acetyl-L-homoserine sulfhydrylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01326: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase
  • amino acids 9 to 426 (418 residues), 597 bits, E=8.8e-184
• PF01053: Cys_Met_Meta_PP
  • amino acids 10 to 427 (418 residues), 418.1 bits, E=5.2e-129
• PF01041: DegT_DnrJ_EryC1
  • amino acids 63 to 188 (126 residues), 42.7 bits, E=8.8e-15
• PF00155: Aminotran_1_2
  • amino acids 67 to 187 (121 residues), 28.6 bits, E=1.7e-10
• PF00266: Aminotran_5
  • amino acids 139 to 220 (82 residues), 23 bits, E=7.2e-09

Best Hits

Swiss-Prot: 51% identical to METY_LEPME: O-acetyl-L-homoserine sulfhydrylase (metY) from Leptospira meyeri

KEGG orthology group: K01740, O-acetylhomoserine (thiol)-lyase [EC: 2.5.1.49] (inferred from 97% identity to azl:AZL_018560)

MetaCyc: 51% identical to O-acetylhomoserine sulfhydrylase (Leptospira meyeri)
O-acetylhomoserine aminocarboxypropyltransferase. [EC: 2.5.1.49]

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48, EC 2.5.1.49)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (9/10 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (12/15 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (5/6 steps found)
• L-homocysteine biosynthesis (2/2 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• L-methionine biosynthesis III (3/4 steps found)
• homocysteine and cysteine interconversion (3/4 steps found)
• L-methionine biosynthesis II (4/6 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (3/5 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (2/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Cysteine metabolism
• Methionine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.48, 2.5.1.49

Use Curated BLAST to search for 2.5.1.48 or 2.5.1.49

Protein Sequence (431 amino acids)

>MPMX19_01517 O-acetyl-L-homoserine sulfhydrylase (Azospirillum sp. SherDot2)
MTEQKSFGFETRAIHAGAAPDPATGARQTPIYQTTSFVFENVDDAASLFNLQKVGFIYSR
LTNPTVAVLEERLANLEGGAGATATSSGHAAQLLALFPLMAPGDHIVASKKLYGGSLNQL
GISFPRAFGWQPSFVDTDTVDNVKAALTEKTKAIFVESLANPGGVVTDIEAIAKIADEAG
IPLIVDNTLATPYLINPIQWGATLVVHSTTKFLSGNGTSVGGVVVDSGKFDWSKSGKFPA
LSEPDPGYHGLRFHETFGHLAFTIHGHAVGLRDLGPSQAPMNAFLTLNGIETLPLRMQRH
ADSALKVATFLESHPAVSWVSYAGLESSKYKELAKKYLPRGAGAVLTFGVKGGFEAGVKM
VESVELFSHLANIGDARSLIIHPSSTTHRQLSAEAQASAGAGPDVIRLSIGLETPEDIIA
DLDQALNKTLG