Protein Info for MPMX19_00586 in Azospirillum sp. SherDot2

Annotation: Nitrogenase molybdenum-iron protein beta chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 519 TIGR01286: nitrogenase molybdenum-iron protein beta chain" amino acids 6 to 519 (514 residues), 833.5 bits, see alignment E=2.9e-255 PF11844: DUF3364" amino acids 6 to 60 (55 residues), 84.4 bits, see alignment 5.4e-28 PF00148: Oxidored_nitro" amino acids 74 to 496 (423 residues), 409.8 bits, see alignment E=1.2e-126

Best Hits

Swiss-Prot: 85% identical to NIFK_AZOBR: Nitrogenase molybdenum-iron protein beta chain (nifK) from Azospirillum brasilense

KEGG orthology group: K02591, nitrogenase molybdenum-iron protein beta chain [EC: 1.18.6.1] (inferred from 98% identity to azl:AZL_007690)

Predicted SEED Role

"Nitrogenase (molybdenum-iron) beta chain (EC 1.18.6.1)" in subsystem Nitrogen fixation (EC 1.18.6.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.18.6.1

Use Curated BLAST to search for 1.18.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (519 amino acids)

>MPMX19_00586 Nitrogenase molybdenum-iron protein beta chain (Azospirillum sp. SherDot2)
MTDKLSQSADKVLDHYTLFRQPEYAAMFEKKKTEFEYGHSDEEVARVSEWTKSEEYKAKN
FAREAVVINPTKACQPIGAMFAAQGFEGTLPFVHGSQGCVAYYRTHLTRHFKEPNSAVSS
SMTEDAAVFGGLNNMIDGLANAYALYKPKMIAVMTTCMAEVIGDDLQGFIANAKTKDSVP
ADFPVPYAHTPAFVGSHIVGYDNMIKGILTNFWGTSENFDTPKTEQINLIPGFDGFAVGN
NRELKRIAGEFGVKLQILSDVSDNFDTPMDGEYRMYDGGTTIEETKEALHAKATISMQEY
NTNQTLQFCKEKGQEVAKFNYPMGVTGTDELLLKLAELSGKPVPASLKLERGRLVDAIAD
SHTHMHGKRFAVYGDPDFCLGMSKFLLELGAEPVHILSTSGSKKWEKQVQKVLDGSPFGA
SGKAHGGKDLWHLRSLIFTDKVDYIIGNSYGKYLERDTKVPLIRLTYPIFDRHHHHRYPT
WGYQGALNVLVRILDRIFEDIDANTNIVGQTDYSFDLIR