Protein Info for MIT1002_02879 in Alteromonas macleodii MIT1002

Annotation: Glutamate-ammonia-ligase adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 977 PF03710: GlnE" amino acids 44 to 295 (252 residues), 263.5 bits, see alignment E=1.9e-82 amino acids 577 to 833 (257 residues), 275.1 bits, see alignment E=5.4e-86 PF08335: GlnD_UR_UTase" amino acids 318 to 456 (139 residues), 101.2 bits, see alignment E=5.2e-33 amino acids 858 to 952 (95 residues), 30.8 bits, see alignment E=2.7e-11

Best Hits

Swiss-Prot: 88% identical to GLNE_ALTMD: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 88% identity to amc:MADE_02610)

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (977 amino acids)

>MIT1002_02879 Glutamate-ammonia-ligase adenylyltransferase (Alteromonas macleodii MIT1002)
MPTDNENSMTNIELAEKFWQQLLTAPAISDSDKAVIEAHCEILMLSFTRSSFLAETLIQN
PSFVSRIVEPYQPPEKSETLQSLGNTMSSDYLANLDNELHNVASEDDLMQVLREFRNKEM
ARITFLDVLNKQPIEISLMQVSALADVLINGAYHWLYQHLAARYGKPQSHNQDMHMYILG
MGKLGGKELNFSSDIDLIFTYPEKGETQGGRKSVEHQQFFTKLAQKLIQALNKVTNDGQV
YRVDMRLRPFGDSGPLVMHFAALEDYYQDQGRHWERFAMVKARVINDDNSQDVKWLKSIL
HPFTFRRYLDFTTLDALRNMKKLIATEIRRRRLSNNIKLGAGGIREVEFFAQSFQLIHGG
REPALQSKSLLRTLDALTELDIVERDVTQQLKSDYLFLRKVEHTLQQAQDRQTQTLPELP
WQQAALIEVIGFDSYSEFLVHLDTVMARIHAHFNELVEESHDTHSEEDQLFTACQDAWRI
SLQEDEFTETFSEYLSASDIKGIYSILVAFKDKQRNYRIGQKGEDTLNKLLPEILCVLIN
QHPNYIPQVLDRLLGVIEAITGRTTYLDLLLENPDVLKQLVRLCERSDWIAQEIKRFPLL
LDELLTPLYLGQQNTDIHTSKQEYQLELREIMLRIEQDDVEMLMDGWRQFKLCQQLRIAA
SDISESLPINNVSDKLTVLAEVILENVVDAAWMQMQQRYGVPAHLEGTDKGFAVIGYGKL
GGYELGYGSDLDLVFLHNAPRGISTDGKKSIEAQQFYIKLAQRIMHLLNTKTLFGQLYET
DLRLRPSGNAGLLCCHIDGFEKYQTEEAWTWEHQALVRARAICGDPRLLNDFTQVRNSIL
TRSRDAKTLADDVCKMRLKMREHLLSKDNDKVDLKQCVGGITDIEFMAQYLVLANAKSQA
AMTEYPDNLRIFDAAAKSNIIDVATAHKLQKAYLKLREQYHHLTLADTKYADQSEELDAI
REQVTIHWNALFGTCSG