Protein Info for MIT1002_00211 in Alteromonas macleodii MIT1002

Annotation: General secretion pathway protein F

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 411 transmembrane" amino acids 177 to 200 (24 residues), see Phobius details amino acids 227 to 247 (21 residues), see Phobius details amino acids 261 to 276 (16 residues), see Phobius details amino acids 284 to 303 (20 residues), see Phobius details amino acids 381 to 402 (22 residues), see Phobius details PF00482: T2SSF" amino acids 75 to 198 (124 residues), 107.5 bits, see alignment E=2.4e-35 amino acids 279 to 400 (122 residues), 94.8 bits, see alignment E=2e-31

Best Hits

Swiss-Prot: 32% identical to PILC_VIBCH: Type IV pilin assembly protein PilC (pilC) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K12278, MSHA biogenesis protein MshG (inferred from 91% identity to amc:MADE_00255)

Predicted SEED Role

"MSHA biogenesis protein MshG" in subsystem Mannose-sensitive hemagglutinin type 4 pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (411 amino acids)

>MIT1002_00211 General secretion pathway protein F (Alteromonas macleodii MIT1002)
MPQFKYAGKSTQGGPQKGIIEAASAQAAAQALLSQNIVPLTIVESKKRSAENEGDGFDIT
RLFQQKVGLDEMIIFSRQMYSLLKAGIPIIRAIKGLSENASHKRFQEILKDIADQLEQGR
SLSSSMAKYEKVFTRLTISVVVVGENTGKLDDVFLQLALYFEREQETRRRIKSALRYPTF
VLIALAIAMFILNLFVVPVFTQMFERFDTELPIMTRVLIGTSNFFVNYWWLLIFVIVGGI
FAVKQYVNSTNGRLKWDKFKLKLPIVGSIIERSLLSRYSRSFSMILRAGVPLTAGLSLTA
DAVDNAHMRKRIKEMRQGIEKGESLLRVSKNSELFSTLVLQMIAVGEETGRLEPLLEESA
DYYEREVDFDLKSLTAKIEPILIGFVAVMVLILALGIFTPMWNMMSAVKGG