Protein Info for GFF994 in Methylophilus sp. DMC18
Annotation: Acetyl-coenzyme A synthetase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 72% identical to ACSA_CUPNH: Acetyl-coenzyme A synthetase (acsA) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)
KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 84% identity to mmb:Mmol_1379)MetaCyc: 72% identical to acetyl-CoA synthetase (Cupriavidus necator)
Propionate--CoA ligase. [EC: 6.2.1.17]; Acetate--CoA ligase. [EC: 6.2.1.17, 6.2.1.1]
Predicted SEED Role
"Acetyl-coenzyme A synthetase (EC 6.2.1.1)" in subsystem Ketoisovalerate oxidoreductase or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 6.2.1.1)
MetaCyc Pathways
- ethanol degradation II (3/3 steps found)
- ethanol degradation IV (3/3 steps found)
- 2-methylcitrate cycle I (4/5 steps found)
- acetate and ATP formation from acetyl-CoA III (1/1 steps found)
- acetate conversion to acetyl-CoA (1/1 steps found)
- L-isoleucine biosynthesis V (2/3 steps found)
- ethanol degradation III (2/3 steps found)
- superpathway of acetate utilization and formation (2/3 steps found)
- L-isoleucine biosynthesis IV (4/6 steps found)
- 2-methylcitrate cycle II (3/6 steps found)
- chitin deacetylation (1/4 steps found)
- superpathway of coenzyme A biosynthesis II (plants) (5/10 steps found)
- reductive glycine pathway of autotrophic CO2 fixation (4/9 steps found)
- β-alanine biosynthesis II (1/6 steps found)
- adlupulone and adhumulone biosynthesis (1/6 steps found)
- colupulone and cohumulone biosynthesis (1/6 steps found)
- lupulone and humulone biosynthesis (1/6 steps found)
- cis-geranyl-CoA degradation (1/9 steps found)
- superpathway of bitter acids biosynthesis (3/18 steps found)
KEGG Metabolic Maps
- Glycolysis / Gluconeogenesis
- Propanoate metabolism
- Pyruvate metabolism
- Reductive carboxylate cycle (CO2 fixation)
Isozymes
Compare fitness of predicted isozymes for: 6.2.1.1
Use Curated BLAST to search for 6.2.1.1 or 6.2.1.17
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (658 amino acids)
>GFF994 Acetyl-coenzyme A synthetase (Methylophilus sp. DMC18) MSIESVLHEHRVFAPGKEFSSQAAIGSMEEYQRLCQEAAQDYEGFWAKLARELLIWHKPF TKTLNQDHAPFYKWFEDGELNVSANCLDKHLQTIPNKTAIIFEADNGDVRSVSYKELYHQ VCQFANGLKSLKLVKGDRVIIYLPMGIEAVVAMQACARLGIIHSVVFGGFSAKSLNERII DAGAVAVITSDGQYRGGKTLPLKQAVDEGIAMGGCDSIRHMVVFKKTGMDVAWDSTCVWW HDLIAGQADTCEPVMVDAEHPLFLLYTSGSTGKPKGVQHSSAGYLLHAMNTMRWTFDIKP NDIYWCTADVGWITGHSYVAYGPLAMGATQIVFEGIPTYPNAGRFWEMIQRHKVSVFYTA PTAIRSLIKAGETDFTTHPNHFDLASLRLLGTVGEPINPEAWMWYYEQVGGNRCPIVDTF WQTETGGHVITPLPGATPLVPGSCTLPFPGIDIDVVDETGKAIAWGNGGLLVIKKPWPSM IRTIYNDPERYKASYFPAELGGNLYLAGDGAVRDADTGNFTIMGRIDDVLNVSGHRLGTM EIESALVANHLVAESAVVGKPHPIKGESVVAYVVLKGKRPEGEEAKKIVAELRDWVGKEI GPIAKPDEIRFGDNLPKTRSGKIMRRLLRSLAKGEEITSDISTLDNPAILEQLKQAVN