Protein Info for GFF1750 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: GTPase and tRNA-U34 5-formylation enzyme TrmE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 454 PF10396: TrmE_N" amino acids 6 to 120 (115 residues), 123.4 bits, see alignment E=1.2e-39 TIGR00450: tRNA modification GTPase TrmE" amino acids 11 to 454 (444 residues), 330.2 bits, see alignment E=2.4e-102 PF12631: MnmE_helical" amino acids 123 to 451 (329 residues), 210.4 bits, see alignment E=7e-66 TIGR00231: small GTP-binding protein domain" amino acids 216 to 370 (155 residues), 80.9 bits, see alignment E=8.8e-27 PF01926: MMR_HSR1" amino acids 218 to 336 (119 residues), 90.4 bits, see alignment E=1.7e-29 PF02421: FeoB_N" amino acids 218 to 306 (89 residues), 30.8 bits, see alignment E=4e-11

Best Hits

Swiss-Prot: 100% identical to MNME_SALTY: tRNA modification GTPase MnmE (mnmE) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03650, tRNA modification GTPase (inferred from 99% identity to sty:STY3937)

MetaCyc: 95% identical to 5-carboxymethylaminomethyluridine-tRNA synthase GTPase subunit (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"GTPase and tRNA-U34 5-formylation enzyme TrmE" in subsystem Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (454 amino acids)

>GFF1750 GTPase and tRNA-U34 5-formylation enzyme TrmE (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MSHNDTIVAQATPPGRGGVGILRISGLKARDVAQEVLGKLPKPRYADYLPFKDVDGSALD
QGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTLPGVRIARPGEFSERAFLNDK
LDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLVEALTHLRIYVEAAIDFPDEE
IDFLSDGKIEAQLNGVIADLDAVRTEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRDASDEVERIGIERAWQEIEQADRVL
FMVDGTTTDAVDPADIWPDFIARLPKNLPITVVRNKADITGETLGISEVNGHSLVRLSAR
TGEGIDVLRNHLKQSMGFDTNMEGGFLARRRHLQALAEAAEHLEQGKAQLLGAWAGELLA
EELRLAQQSLSEITGEFTSDDLLGRIFSSFCIGK