Protein Info for Psest_1603 in Pseudomonas stutzeri RCH2

Annotation: Thiol:disulfide interchange protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 578 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details transmembrane" amino acids 164 to 193 (30 residues), see Phobius details amino acids 206 to 229 (24 residues), see Phobius details amino acids 241 to 264 (24 residues), see Phobius details amino acids 285 to 314 (30 residues), see Phobius details amino acids 321 to 345 (25 residues), see Phobius details amino acids 361 to 379 (19 residues), see Phobius details amino acids 385 to 403 (19 residues), see Phobius details amino acids 412 to 433 (22 residues), see Phobius details PF11412: DsbD_N" amino acids 28 to 136 (109 residues), 101.1 bits, see alignment E=1.3e-32 PF02683: DsbD_TM" amino acids 165 to 378 (214 residues), 265.8 bits, see alignment E=7.4e-83 PF13386: DsbD_2" amino acids 170 to 358 (189 residues), 38.8 bits, see alignment E=2.8e-13 PF13899: Thioredoxin_7" amino acids 468 to 545 (78 residues), 48.9 bits, see alignment E=1.8e-16 PF13098: Thioredoxin_2" amino acids 475 to 569 (95 residues), 31.9 bits, see alignment E=4.2e-11 PF00085: Thioredoxin" amino acids 478 to 547 (70 residues), 25.6 bits, see alignment E=3.1e-09

Best Hits

Swiss-Prot: 67% identical to DSBD2_PSEAE: Thiol:disulfide interchange protein DsbD 2 (dsbD2) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K04084, thiol:disulfide interchange protein DsbD [EC: 1.8.1.8] (inferred from 93% identity to psa:PST_2708)

Predicted SEED Role

"Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8)" in subsystem Biogenesis of c-type cytochromes or Periplasmic disulfide interchange (EC 1.8.1.8)

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.8

Use Curated BLAST to search for 1.8.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GHE2 at UniProt or InterPro

Protein Sequence (578 amino acids)

>Psest_1603 Thiol:disulfide interchange protein (Pseudomonas stutzeri RCH2)
MRALLLMLLLLLPGLALPAGGLFGGSAQQDFLPVEEAFALTVSPQDNGATLLHWRIAPGY
YLYQQRLQFDGLPPERQPQLPEGQPYSDEFFGDSQIYRDSLELIIPAGAENNVRLGWQGC
ADAGLCYPPQTREVSLSGDGSASAASGQARDQALASGLAGQALGWSLLVFFGLGLLLAFT
PCSLPMLPILAGIVVGSGATSRRGFALASTYVVSMALVYAGLGVLAALLGANLQAALQQP
WLLTSFAVLFVLLALPMFGLFELQLPVALRDRLQRVGDRQRGGNLAGAGALGVLSGLLVG
PCMTAPLAAALLFIAQSGSALQGGLVLFALGLGIGTPLLLLVTVGQRFLPKPGAWMDRVK
VVFGFLFLAAALFVARPLLADWLWLGLWGALLVVLATALLNVAQAVQRQRVLCQASGVLL
GLWGAAMLLGAAGGADDPRRPLAIYAGGGADAISAEVHALGFSDPAVLDRELAAAKAQGQ
WVLIDYYADWCVSCKVMEKEVFGNAEVQASLDGVRVLRPDVTRSGAASRELLDRYQVLGP
PTLVWIGPDGVERRERRITGEVGAEGFLQNWNQTRERG