Protein Info for GFF1284 in Sphingobium sp. HT1-2

Annotation: Inner membrane protein, KefB/KefC family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 transmembrane" amino acids 13 to 32 (20 residues), see Phobius details amino acids 39 to 59 (21 residues), see Phobius details amino acids 65 to 83 (19 residues), see Phobius details amino acids 95 to 116 (22 residues), see Phobius details amino acids 122 to 141 (20 residues), see Phobius details amino acids 161 to 180 (20 residues), see Phobius details amino acids 192 to 213 (22 residues), see Phobius details amino acids 228 to 245 (18 residues), see Phobius details amino acids 249 to 268 (20 residues), see Phobius details amino acids 279 to 299 (21 residues), see Phobius details amino acids 304 to 328 (25 residues), see Phobius details amino acids 335 to 355 (21 residues), see Phobius details amino acids 366 to 388 (23 residues), see Phobius details PF00999: Na_H_Exchanger" amino acids 26 to 385 (360 residues), 193.4 bits, see alignment E=5.5e-61 PF02254: TrkA_N" amino acids 414 to 521 (108 residues), 63.3 bits, see alignment E=2.7e-21

Best Hits

Swiss-Prot: 38% identical to KEFX_HAEIN: Glutathione-regulated potassium-efflux system protein (kefBC) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: None (inferred from 87% identity to sjp:SJA_C1-22830)

Predicted SEED Role

"putative Glutathione-regulated potassium-efflux system protein KefB" in subsystem Potassium homeostasis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (600 amino acids)

>GFF1284 Inner membrane protein, KefB/KefC family (Sphingobium sp. HT1-2)
MPVVETAMSATDVLLSEGVILLGVAVAFVLLFRRFGLGAVLGYLVAGALVGPQGLGLVGG
AESKLAIAEIGIVLLLFLVGLELHPARLWRLKRDIFALGLAQVVLCGLALIAIIFYSTGF
TWGAAIALGLPLALSSTAQVLPSLKSSGRINSPFGEKVFSILLFQDLSIVPLITIVAALS
RNPADAGGPPGWVLAGYTVAAIAGLVLAGRFILRPLLNLVGRQGERELFVAVGLFTVLAA
ASVMHSLHLSTALGAFVAGVMLADSPYRHEIESDVEPFRSILLGLFFLAVGMVLDLRAVA
ANPLFVIGMAAMLVATKAAIITLLARLFGMAWQQALSAGLLLSQGGEFGFVLFAQAQNAY
LIAPQAASLFSAIVTFSMATTPFLMLFARRFEFAQPKDRDDLPGPEDAPRGSAIIVGYGR
FGQTVAQMLMGHGFGVVLIDKKPAQIEVSSRFDMKVYYGDGTRIDLLRRAGADEARLIAF
CIDDPSLDGRVLQPIAEAFPQAALMARVFDRRQILAMQDIELAGVVREVFESAICMGVQA
MQTLGVPPEEVEEVERQYRENDGQRLALQIEHGNLLAAKDLMYRPGRGMRLRARGDGEEA