Protein Info for EX28DRAFT_0184 in Enterobacter asburiae PDN3

Annotation: phosphoribosylaminoimidazole-succinocarboxamide synthase (EC 6.3.2.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 98% identical to PUR7_ENT38: Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) from Enterobacter sp. (strain 638)

KEGG orthology group: K01923, phosphoribosylaminoimidazole-succinocarboxamide synthase [EC: 6.3.2.6] (inferred from 96% identity to ecl:EcolC_1200)

MetaCyc: 96% identical to phosphoribosylaminoimidazole-succinocarboxamide synthase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylaminoimidazolesuccinocarboxamide synthase. [EC: 6.3.2.6]

Predicted SEED Role

"Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC 6.3.2.6)" in subsystem De Novo Purine Biosynthesis (EC 6.3.2.6)

MetaCyc Pathways

superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
inosine-5'-phosphate biosynthesis I (6/6 steps found)
inosine-5'-phosphate biosynthesis II (5/5 steps found)
inosine-5'-phosphate biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (237 amino acids)

>EX28DRAFT_0184 phosphoribosylaminoimidazole-succinocarboxamide synthase (EC 6.3.2.6) (Enterobacter asburiae PDN3)
MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFDRKGMVNNKFNHFIMTK
LAEAGIPTQMEALLSDTECLVKKLDMVPVECVIRNRAAGSLVKRLGIEEGIELNPPLFDL
FLKNDAMHDPMVNESYCETFGWVSKENLARMQELTYKANDVLKKLFDDAGLILVDFKLEF
GLFKGEVVLGDEFSPDGSRLWDKETLDKMDKDRFRQSLGGLIEAYEAVAHRLGVKLD