Protein Info for BWI76_RS20600 in Klebsiella michiganensis M5al

Annotation: nucleoside permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 418 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details amino acids 42 to 62 (21 residues), see Phobius details amino acids 71 to 89 (19 residues), see Phobius details amino acids 95 to 116 (22 residues), see Phobius details amino acids 135 to 154 (20 residues), see Phobius details amino acids 160 to 179 (20 residues), see Phobius details amino acids 211 to 231 (21 residues), see Phobius details amino acids 254 to 275 (22 residues), see Phobius details amino acids 282 to 300 (19 residues), see Phobius details amino acids 306 to 327 (22 residues), see Phobius details amino acids 347 to 369 (23 residues), see Phobius details amino acids 382 to 402 (21 residues), see Phobius details TIGR00889: nucleoside transporter" amino acids 1 to 418 (418 residues), 688.2 bits, see alignment E=2.3e-211 PF03825: Nuc_H_symport" amino acids 1 to 406 (406 residues), 594.7 bits, see alignment E=1.9e-182 PF12832: MFS_1_like" amino acids 6 to 373 (368 residues), 85.6 bits, see alignment E=7.5e-28 PF01306: LacY_symp" amino acids 9 to 403 (395 residues), 31.3 bits, see alignment E=2e-11 PF07690: MFS_1" amino acids 254 to 409 (156 residues), 46.3 bits, see alignment E=5.7e-16

Best Hits

Swiss-Prot: 85% identical to XAPB_ECOLI: Xanthosine permease (xapB) from Escherichia coli (strain K12)

KEGG orthology group: K11537, MFS transporter, NHS family, xanthosine permease (inferred from 90% identity to set:SEN2402)

MetaCyc: 85% identical to xanthosine:H+ symporter XapB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-31

Predicted SEED Role

"Xanthosine permease" in subsystem Xanthosine utilization (xap region)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B7H1 at UniProt or InterPro

Protein Sequence (418 amino acids)

>BWI76_RS20600 nucleoside permease (Klebsiella michiganensis M5al)
MGIASRLKLMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVYSSKGLAAIIMPGVMGI
IADKWLRAERAYMLCHLVCAGVLLYATTVTDPDTMFWVMLINAMAYMPTISLSNSVSYSC
LAQSGQDPATAFPPVRVFGTLGFIVAMWMVSLMGLELSSAQLYIASAASLLLALYAFTLP
KIPVTEKKASTTLAARLGLDAFVLFKNPRMAIFFFFAMMLGAVLQITNVFGNPFLHDFAR
NPAFADSFVVRYPSILLSVSQMAEVAFILTIPFFLKRFGIKTVMLMSMLAWTLRFGFFAF
GDPSPFGFVLLLMSMIVYGCAFDFFNISGSVFVEQQVGANIRASAQGLFMTMVNGVGAWV
GSVLSGLAVDYFSLDGVKDWQTIWLVFAAYSLMLAVIFALFFRYKHEPEQRMEKELAH