Protein Info for BWI76_RS03085 in Klebsiella michiganensis M5al

Updated annotation (from data): 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29)
Rationale: Specifically important in carbon source m-Inositol. This protein is a fusion of 5-keto-deoxygluconate kinase and DUF2090. As DUF2090 has distant similarity to aldolases, it may provide the missing 5-dehydro-2-deoxyphosphogluconate aldolase activity.
Original annotation: 5-dehydro-2-deoxygluconokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 637 TIGR04382: 5-dehydro-2-deoxygluconokinase" amino acids 8 to 332 (325 residues), 415.9 bits, see alignment E=5e-129 PF00294: PfkB" amino acids 9 to 324 (316 residues), 153.6 bits, see alignment E=8e-49 PF09863: DUF2090" amino acids 327 to 635 (309 residues), 450.4 bits, see alignment E=3e-139

Best Hits

KEGG orthology group: None (inferred from 93% identity to eae:EAE_09810)

MetaCyc: 51% identical to bifunctional 5-dehydro-2-deoxygluconokinase/5-dehydro-2-deoxyphosphogluconate aldolase (Caulobacter vibrioides NA1000)
5-dehydro-2-deoxyphosphogluconate aldolase. [EC: 4.1.2.29]; 5-dehydro-2-deoxygluconokinase. [EC: 4.1.2.29, 2.7.1.92]

Predicted SEED Role

"5-keto-2-deoxygluconokinase (EC 2.7.1.92) / uncharacterized domain" in subsystem Inositol catabolism (EC 2.7.1.92)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.92 or 4.1.2.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AX19 at UniProt or InterPro

Protein Sequence (637 amino acids)

>BWI76_RS03085 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29) (Klebsiella michiganensis M5al)
MNAAVKRLDVICIGRVAVDLYAQQIGARLEDVASFSKYLGGSSGNVAFGTAIQGLKSAML
ARVGDEHNGRFLRETLSRAGVDTEYLITDKQRLTALVMLGIKDQETFPLIFYRDNCADMA
LSPEDIKEEYIASSRALAVTGTHLSHANTREAVLKALEYARRHGLRTALDIDYRPVLWGL
TSLGDGETRFIESGQVTSQLQEVLHLFDLVVGTEEEFHIAGGSTDTLTALKNVRNATKAT
LVCKRGPMGCVVLEGAIPDNWDSVPLQQGVRVDVLNVLGAGDAFMSGLLRGWLNDEGWEQ
ACRYANACGALVVSRHGCAPAMPTKAELDDYLSRAESVPRPDIDDRLNHLHRVTSRRQAW
PELCIFAFDHRKQLADLALETGRDESCIPQLKLLLLAAAEAAADEAGLDGRSGILADGTY
GQRSLNAITGKGWWIGRPIEMPSSRPLRLEHGNIGSQLIDWPLEHVVKCLVFYHPADPAE
LRAEQDALLLEVWQACNKSGHELLLEIILPENGPDKDERHYHDMLEHFYQLGIQPDWWKL
PPLSSAEWERIGKLIAREDSWCRGILILGLDAPSDRLRAGFAEAAKHPMIKGFAVGRTIF
GQPSRRWMQGELSDEALINEVKRNYLTLIGYWREARG