Protein Info for AO353_05065 in Pseudomonas fluorescens FW300-N2E3

Annotation: phosphate acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13500: AAA_26
  • amino acids 2 to 213 (212 residues), 115.4 bits, E=5e-37
• PF07085: DRTGG
  • amino acids 217 to 327 (111 residues), 94 bits, E=7.3e-31
• PF01515: PTA_PTB
  • amino acids 374 to 689 (316 residues), 376.6 bits, E=1.8e-116
• TIGR00651: phosphate acetyltransferase
  • amino acids 389 to 689 (301 residues), 377.6 bits, E=2.6e-117

Best Hits

Swiss-Prot: 87% identical to PTA_PSEPK: Phosphate acetyltransferase (pta) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K13788, phosphate acetyltransferase [EC: 2.3.1.8] (inferred from 97% identity to pfl:PFL_0938)

Predicted SEED Role

"Phosphate acetyltransferase (EC 2.3.1.8)" in subsystem Ethanolamine utilization or Fermentations: Lactate or Fermentations: Mixed acid or MLST or Propanediol utilization or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate or Threonine anaerobic catabolism gene cluster (EC 2.3.1.8)

MetaCyc Pathways

• heterolactic fermentation (16/18 steps found)
• ethanolamine utilization (5/5 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (4/4 steps found)
• pyruvate fermentation to acetate II (3/3 steps found)
• superpathway of acetate utilization and formation (3/3 steps found)
• acetate and ATP formation from acetyl-CoA I (2/2 steps found)
• mixed acid fermentation (12/16 steps found)
• acetylene degradation (anaerobic) (4/5 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
• pyruvate fermentation to acetate and lactate II (3/4 steps found)
• pyruvate fermentation to acetate I (2/3 steps found)
• pyruvate fermentation to acetate IV (2/3 steps found)
• pyruvate fermentation to acetate VII (2/3 steps found)
• L-threonine degradation I (4/6 steps found)
• sulfoacetaldehyde degradation I (1/2 steps found)
• superpathway of N-acetylneuraminate degradation (15/22 steps found)
• superpathway of L-threonine metabolism (12/18 steps found)
• acetyl-CoA fermentation to butanoate (4/7 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (5/9 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (5/9 steps found)
• (S)-propane-1,2-diol degradation (2/5 steps found)
• sulfolactate degradation II (1/4 steps found)
• methanogenesis from acetate (2/6 steps found)
• superpathway of sulfolactate degradation (2/6 steps found)
• superpathway of taurine degradation (2/6 steps found)
• superpathway of L-alanine fermentation (Stickland reaction) (4/9 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (3/8 steps found)
• L-lysine fermentation to acetate and butanoate (3/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (8/17 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (5/13 steps found)
• gallate degradation III (anaerobic) (3/11 steps found)
• superpathway of L-lysine degradation (18/43 steps found)
• superpathway of methanogenesis (2/21 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Pyruvate metabolism
• Taurine and hypotaurine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.8

Sequence Analysis Tools

See A0A0N9VKS2 at UniProt or InterPro

Protein Sequence (699 amino acids)

>AO353_05065 phosphate acetyltransferase (Pseudomonas fluorescens FW300-N2E3)
MQTFFIAPTDFGVGLTSISLGLVRTLERAGLKVGFFKPIAQPHPGDTGPERSTELVARTH
GLKPPQPLGLAHVERMLGDGQLDELLEEIITLYQQAAIGKDVLVVEGMVPTRSASYAARV
NLHLAKSLDAEVILVSAPENEVLTELSGRVELQAQLFGGPKDPKVLGVILNKVKTDESME
AFAARLKEHSPLLRSGDFRLLGCIPFQPELNAPRTRDVADLMGAQVLNAGDYETRRMSKI
IICARTMRNTVELLKPGVLVVTPGDRDDIILAVSLAAINGVPLAGLLLTSGTLPDPRIMD
LCRGALQAGLPVLSVSTGSYETANQLNGLNKEIPIDDRERAEIITDFVASHLDANWLHQR
CGTPREMRLSPAVFRYQLIQRAQAANKRIVLPEGSEPLTVQAAAICQARGIARCVLLAKP
EDVQAVARAQGIELPEGLEILDPDLIRQRYVEPMVALRKTKSLNAPMAEQQLEDTVVIGT
MMLALDEVDGLVSGVIHSTANTIRPALQLIKTAPGCTLVSSVFFMLFPEEVLVYGDCIMN
PHPSASELAEIALQSADSAAAFGITPRVAMLSYSSGESASGEEVEKVREATLLAHEAQNA
LLIDGPLQYDAAANENVARQLAPNSQVAGRATVFIFPDLNTGNTTHKAVQRSADCVSLGP
MLQGLRKPVNDLPRGAQVDDIVYTIALTAIQAANRPMDV