Protein Info for Xcc-8004.2957.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00657: aspartate kinase
  • amino acids 20 to 474 (455 residues), 355.6 bits, E=2.4e-110
• PF00696: AA_kinase
  • amino acids 20 to 300 (281 residues), 138.6 bits, E=8.4e-44
• PF13840: ACT_7
  • amino acids 328 to 389 (62 residues), 31.1 bits, 5.3e-11
  • amino acids 409 to 469 (61 residues), 33.4 bits, 1e-11
• PF22468: ACT_9
  • amino acids 334 to 393 (60 residues), 70.1 bits, 3.2e-23
  • amino acids 415 to 474 (60 residues), 77.2 bits, 2e-25
• PF03447: NAD_binding_3
  • amino acids 487 to 621 (135 residues), 68.1 bits, E=3.4e-22
• PF00742: Homoserine_dh
  • amino acids 629 to 825 (197 residues), 165.8 bits, E=2.6e-52

Best Hits

KEGG orthology group: K12524, bifunctional aspartokinase / homoserine dehydrogenase 1 [EC: 1.1.1.3 2.7.2.4] (inferred from 100% identity to xcb:XC_2389)

Predicted SEED Role

"Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3)" in subsystem Lysine Biosynthesis DAP Pathway or Threonine and Homoserine Biosynthesis or Methionine Biosynthesis (EC 1.1.1.3, EC 2.7.2.4)

MetaCyc Pathways

• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• aspartate superpathway (21/25 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of L-threonine biosynthesis (6/6 steps found)
• L-lysine biosynthesis III (6/7 steps found)
• L-lysine biosynthesis VI (6/7 steps found)
• L-homoserine biosynthesis (3/3 steps found)
• L-lysine biosynthesis II (7/9 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• dipicolinate biosynthesis (3/4 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• L-methionine biosynthesis IV (2/4 steps found)
• spermidine biosynthesis II (2/4 steps found)
• ectoine biosynthesis (2/5 steps found)
• 3-dehydroquinate biosynthesis II (archaea) (3/7 steps found)
• norspermidine biosynthesis (2/6 steps found)
• cremeomycin biosynthesis (2/7 steps found)
• grixazone biosynthesis (2/8 steps found)
• superpathway of polyamine biosynthesis III (2/8 steps found)
• platensimycin biosynthesis (6/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of plant hormones
• Glycine, serine and threonine metabolism
• Lysine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.3, 2.7.2.4

Use Curated BLAST to search for 1.1.1.3 or 2.7.2.4

Sequence Analysis Tools

See A0A0H2X7W2 at UniProt or InterPro

Protein Sequence (835 amino acids)

>Xcc-8004.2957.1 Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3) (Xanthomonas campestris pv. campestris strain 8004)
MSSPVVSFEPAAVPTPSARTVVHKFGGTSVADAERYRHVAQLLLARDETVQVTVVSAMKG
VTDALIELAELAAQNRPEWRERWHETRARHRGAAVALLGEHSGPTVEWLDERFEHLSQIL
AALAVIGELPREVLDRVQGLGEVYSAQLLGDHFRALGEDCAVLDARDVLVVNRGELGVDV
DWAVSAQRLDTWRQAHPQTRVVVTGFVARDRGDRITTLGRNGSDYSGAIFAALFDAHELH
IWTDVDGVLSADPRVVPEAVQLETLSYDEACELAYFGAKVVHPQTMSPAIERGLPIIIRN
TFQPEHPGTRISASSAVSGPIKGLTLSPDLAVLNLEGTGLIGVPGTAERVFAALRTAQVS
VVMISQGSSEHSICCVVKQHESERARNALLQAFAHELTVGQVQRVQLTTGISVLAAVGDG
MAGQPGVAARLFESLGRAQVNILAIAQGSSERNISVAIDAAHATKALRAAHAGFWLSPQT
FSVGVIGPGNVGAALLDQLRTAQPQLLGKANLDLRLRGVVSRGRMLLEARSVTGDWRDAF
AAASTPTDLERFTAHLLSAHLPHTVIIDCSGSAEVADRYAGWLAAGIHVVTPNKQAGSGP
LARYEAIRAAADASGARFRYEATVGAGLPVITTLRDLVDTGDAVTSIEGIFSGTLAWLFN
KYDGSVPFSELVTQARGMGYTEPDPRDDLSGVDVARKLVILAREAGRAISLDDVSVESLV
PEALRQASVDDFMAQLQTVDDGFAQRLADAHARGNVLRYVAQLPPDRAPSVGLVELPAHH
AFANLRLTDNVVQFTTRRYCENPLVVQGPGAGPEVTAAGVFADLLRVAAGEGARL