Protein Info for Psyr_4407 in Pseudomonas syringae pv. syringae B728a

Annotation: phosphoribosylamine--glycine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02844: GARS_N
  • amino acids 1 to 101 (101 residues), 125.6 bits, E=2.4e-40
• TIGR00877: phosphoribosylamine--glycine ligase
  • amino acids 1 to 422 (422 residues), 587.4 bits, E=7.6e-181
• PF01071: GARS_A
  • amino acids 102 to 295 (194 residues), 278.4 bits, E=6.6e-87
• PF02786: CPSase_L_D2
  • amino acids 108 to 203 (96 residues), 22.5 bits, E=1.5e-08
• PF02843: GARS_C
  • amino acids 331 to 420 (90 residues), 110 bits, E=1.2e-35

Best Hits

Swiss-Prot: 96% identical to PUR2_PSESM: Phosphoribosylamine--glycine ligase (purD) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K01945, phosphoribosylamine--glycine ligase [EC: 6.3.4.13] (inferred from 100% identity to psb:Psyr_4407)

MetaCyc: 74% identical to phosphoribosylamine--glycine ligase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylamine--glycine ligase. [EC: 6.3.4.13]

Predicted SEED Role

"Phosphoribosylamine--glycine ligase (EC 6.3.4.13)" in subsystem De Novo Purine Biosynthesis (EC 6.3.4.13)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• superpathway of 5-aminoimidazole ribonucleotide biosynthesis (6/6 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis I (5/5 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis II (5/5 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.13

Sequence Analysis Tools

See Q4ZN35 at UniProt or InterPro

Protein Sequence (431 amino acids)

>Psyr_4407 phosphoribosylamine--glycine ligase (Pseudomonas syringae pv. syringae B728a)
MNILIIGSGGREHALAWKVAQDPRVQKVFVAPGNAGTAIEAKCENVAIDVLALEQLADFA
EKNVALTIVGPEVPLVAGVVDLFRSRGLDCFGPTAGAAQLEGSKAFTKDFLARHKIPTAD
YQNFTEIEPALAYLREKGAPIVIKADGLAAGKGVIVAMTLAEAEDAVRDMLAGNAFGDAG
SRVVIEEFLDGEEASFIVMVDGKNVLPMATSQDHKRVGDGDSGPNTGGMGAYSPAPVVTA
EVHQRVMDLVIWPTVRGMADEGNVYTGFLYAGLMIDKAGNPKVIEFNCRFGDPETQPVML
RLQSSLVLLVEAALAQALDKIEAQWDPRPSLGIVLAAGGYPGDYAKGAVIEGLDAAAQLE
GKIFHAGTALQDERVVTSGGRVLCATALGDSVGNAQENAYALAAKVDWQGCFYRKDIGYR
AIARERGEDQE