Protein Info for Psyr_1091 in Pseudomonas syringae pv. syringae B728a

Annotation: aminopeptidase A, Metallo peptidase, MEROPS family M17

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 PF02789: Peptidase_M17_N" amino acids 19 to 147 (129 residues), 117.1 bits, see alignment E=5.1e-38 PF00883: Peptidase_M17" amino acids 184 to 486 (303 residues), 438.5 bits, see alignment E=1.5e-135

Best Hits

Swiss-Prot: 100% identical to AMPA_PSEU2: Probable cytosol aminopeptidase (pepA) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K01255, leucyl aminopeptidase [EC: 3.4.11.1] (inferred from 100% identity to psb:Psyr_1091)

Predicted SEED Role

"Cytosol aminopeptidase PepA (EC 3.4.11.1)" (EC 3.4.11.1)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.11.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZXH7 at UniProt or InterPro

Protein Sequence (496 amino acids)

>Psyr_1091 aminopeptidase A, Metallo peptidase, MEROPS family M17 (Pseudomonas syringae pv. syringae B728a)
MELVVKSVSPETLKTATLVVTVGESRVLAGAARTVDILSGGAVSLILKRGDLAGKVGQSL
LLHNLPNIKAERVLLVGTGKEDELSDRQLKKIVGAALTCLKGLGGTDAAIALDDLSVKNR
DTYGMARLLVEALADGEYVFDRFKTQKAEVRALKKITLLTDKVKAADVERASTHAQAIAT
GMALTRDLGNLPPNICHPTYLGEEAKALGKAHKNLKVEVHDEKKLADLGMGSFLAVAQGS
AQPPRLIVMNYQGGKKGDQPFVLVGKGITFDTGGISIKPASGMDEMKFDMCGAASVFGTL
RAVLELKLPINLVCILACAENMPSGTATRPGDIVTTMSGQTVEILNTDAEGRLVLCDALT
YAERFKPQAVIDIATLTGACVVALGGHTSGLLGNNDALINQLLDAGKQADDRAWQLPLFD
EYQEQLDSPFADIANIGGPKGGTITAACFLSRFTKAYHWAHLDIAGTAWLSGGKEKGATG
RPVPLLTQYLLDRAGV