Protein Info for PfGW456L13_874 in Pseudomonas fluorescens GW456-L13

Annotation: Polyphosphate kinase (EC 2.7.4.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 742 transmembrane" amino acids 470 to 486 (17 residues), see Phobius details TIGR03705: polyphosphate kinase 1" amino acids 59 to 732 (674 residues), 940.3 bits, see alignment E=3.4e-287 PF13089: PP_kinase_N" amino acids 59 to 164 (106 residues), 123.3 bits, see alignment E=1.3e-39 PF02503: PP_kinase" amino acids 175 to 356 (182 residues), 205.9 bits, see alignment E=1.1e-64 PF17941: PP_kinase_C_1" amino acids 383 to 546 (164 residues), 233.2 bits, see alignment E=3.2e-73 PF13090: PP_kinase_C" amino acids 555 to 723 (169 residues), 222 bits, see alignment E=8.8e-70

Best Hits

Swiss-Prot: 92% identical to PPK1_PSESM: Polyphosphate kinase (ppk) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00937, polyphosphate kinase [EC: 2.7.4.1] (inferred from 96% identity to pfo:Pfl01_5464)

Predicted SEED Role

"Polyphosphate kinase (EC 2.7.4.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism or Purine conversions (EC 2.7.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A293QJI3 at UniProt or InterPro

Protein Sequence (742 amino acids)

>PfGW456L13_874 Polyphosphate kinase (EC 2.7.4.1) (Pseudomonas fluorescens GW456-L13)
MNTEVLTEVAVKDAQPVVEQVDETPPELEPAPPAAVVETAVTAPAPVLAIPGLDDSSLYI
HRELSQLQFNIRVLEQALDESYPLLERLKFLLIFSSNLDEFFEIRVAGLKKQITFAREQA
GADGLQPHQALARISELVHGHVDRQYAILNDILLPELEKHQVRFIRRRNWTAKLKTWVRR
YFRDEIAPIITPIGLDPTHPFPLLVNKSLNFIVELEGIDAFGRDSGLAIIPAPRLLPRII
KVPEEVGGAGDNYVFLSSMIHAHADDLFQGMKVKGCYQFRLTRNADLALDSEDVEDLARA
LRGELFSRRYGDAVRLEVADTCPKHLSDYLLKQFNLSETELYQVNGPVNLTRLFSITGLD
SQRELQYLPFTPQIPKLLQNSENIFSVISKQDILLLHPFESFTPVVDLLRQAAKDPHVLA
VRQTLYRSGANSEIVDALVDAARNGKEVTAVIELRARFDEESNLQLASRLQAAGAVVIYG
VVGFKTHAKMMLILRREAGEIVRYAHLGTGNYHAGNARLYTDYSLLTSDDALCEDVGKLF
SQLIGMGKTLRMKKLLHAPFTLKKGMLDMIARETQFALDGKPAHIIAKFNSLTDPKIIRA
LYKASQSGVRIDLVVRGMCCLRPGIAGVSHNIHVRSIIGRFLEHTRVFYFLNGGDEQMFL
SSADWMERNLDKRVETCFPVEGKKLIMRVKKELELYLTDNTHSWSLQSDGRYIRNTPTGN
QNPRSAQATLLERLGSPILAVR