Protein Info for Pf6N2E2_4823 in Pseudomonas fluorescens FW300-N2E2

Annotation: Phospholipase/lecithinase/hemolysin

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 657 signal peptide" amino acids 1 to 47 (47 residues), see Phobius details PF00657: Lipase_GDSL" amino acids 55 to 337 (283 residues), 56.7 bits, see alignment E=6e-19 TIGR01414: outer membrane autotransporter barrel domain" amino acids 353 to 657 (305 residues), 44.6 bits, see alignment E=5e-16 PF03797: Autotransporter" amino acids 387 to 637 (251 residues), 163.7 bits, see alignment E=1.1e-51

Best Hits

Swiss-Prot: 66% identical to ESTA_PSEPU: Esterase EstA (estA) from Pseudomonas putida

KEGG orthology group: K12686, outer membrane lipase/esterase (inferred from 96% identity to pba:PSEBR_a5120)

Predicted SEED Role

"Phospholipase/lecithinase/hemolysin"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A281 at UniProt or InterPro

Protein Sequence (657 amino acids)

>Pf6N2E2_4823 Phospholipase/lecithinase/hemolysin (Pseudomonas fluorescens FW300-N2E2)
MPEVFGTVVSFSNKNKIKTDFPMIKKTLFVPIAGCLLAMACAQASAAPNPYSKFIVFGDS
LNDAGQFADPNGPPGSTERYTNRTGPIYTDGSGEAYSANSTQLLGGRLGYSADETAASTS
AVRADQGLADGNNWAVGGYRTAQILESITGVSDTGERSRPGYLQSNGLRADPNALYYISG
GGNDFLQGLVLTPEQASKAADRLASSVQALQTAGARYVMVWLLPDLGLTPFLNGSPSQDF
FSQLGNQFNQQLITRLQGIDAEIIPLNIPLLLKESFADPARFGLAIGENLTATCFTGNGC
TENATYGINSATPDPTKLIYNDAVHPTEAGQKLIADYAYSLLAAPWELTLLPEMAHSTLR
AHQDELRSQWQSDWENWQAVGQWRAIVAGGGQHLDMDSQDSGASADGSGYSLNIGGSYRL
NDAWRVGVAAGLYRQDMEAGHNDSKYKLNSYLATAFAQFQQNRWWADAALTGGKLDYDNL
ERKFDLGVNEAQEKGDTDGHLWAFSTRVGYDIAQPGSEWHLSPFISADYAKVEVDGYSEK
SNRSTALTFDDQTRDSKRLGIGLQGKYNITRQTQVFGEYAHEREYEDDTQKVNIALNSLP
ANDFTLEGYTPQSHLNRLSLGVSHKLTNDLALRGGYTLRKDDDMTQQGVSLGVSLDF