Protein Info for Pf6N2E2_1314 in Pseudomonas fluorescens FW300-N2E2

Annotation: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 265 TIGR02311: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase" amino acids 6 to 254 (249 residues), 435.3 bits, see alignment E=3.3e-135 PF03328: HpcH_HpaI" amino acids 18 to 243 (226 residues), 198.8 bits, see alignment E=3.7e-63

Best Hits

Swiss-Prot: 68% identical to HPCH_ECOHS: 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH) from Escherichia coli O9:H4 (strain HS)

KEGG orthology group: K02510, 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase [EC: 4.1.2.-] (inferred from 99% identity to pba:PSEBR_a2737)

MetaCyc: 67% identical to 4-hydroxy-2-ketopimelate aldolase monomer (Escherichia coli C)
4-HYDROXY-2-KETOPIMELATE-LYSIS-RXN [EC: 4.1.2.52]

Predicted SEED Role

"2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4)" (EC 4.1.2.n4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.2.-, 4.1.2.n4

Use Curated BLAST to search for 4.1.2.- or 4.1.2.52 or 4.1.2.n4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZT77 at UniProt or InterPro

Protein Sequence (265 amino acids)

>Pf6N2E2_1314 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4) (Pseudomonas fluorescens FW300-N2E2)
MDMPINTFKQRLRSGEAQIGLWLGLADAYCAELAANAGFDWLLIDGEHAPNDLRTLLGQL
QAVAPYPSQPVIRPVIGDTALIKQVLDIGVQTLLVPMVESADQARELVRAIHYPPHGVRG
VGSALARASRWNSIPGYLDKADEQMCLLVQIESREGLANLDAIAAVDGVDGVFIGPADLS
ASMGFRGNPGHPQVQAAIEDAIARIRQAGKAAGILSADEKLARRYIELGAAFVAVGVDTT
VLMRGLQTLAATFKDTPKPAAGGVY