Protein Info for OKGIIK_03915 in Rhodanobacter sp. FW510-T8

Annotation: N-acetylmuramoyl-L-alanine amidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 506 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF01520: Amidase_3" amino acids 198 to 414 (217 residues), 165.7 bits, see alignment E=1.1e-52 PF01476: LysM" amino acids 462 to 504 (43 residues), 48.8 bits, see alignment 5.3e-17

Best Hits

Predicted SEED Role

"N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)" (EC 3.5.1.28)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.28

Use Curated BLAST to search for 3.5.1.28

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (506 amino acids)

>OKGIIK_03915 N-acetylmuramoyl-L-alanine amidase (Rhodanobacter sp. FW510-T8)
MRPMRGCLNNWGGVVAVAIMAALPLCALRAADVKGARVWAGPEYTRVVLDATGPLRYTVD
RKDGQVVVDLPGSRAARDFSSPAARGLFRGMSHARIGDSLQLTAQVAPASKLKSFVLKPA
SGSDYRLVLDLYPGNDGAMASVAEVAPKAADKAAPPPVAAVPAAATAPVPQYSNPTHSRR
VAAEQAAAMLNGQRQVVVAIDAGHGGKDQGAHGPGGTLEKNVTLSVARNLAAEINRQPGM
KAVLTRDSDYFIPLKRRYQIAREHNADLFVSIHADSFVNDDARGSSVWVLSPRGKTSEAA
RWLADRENRADLIGGTTLDDKDDSLAKVLLDLQQGWAMQASDVVAGNVLKALGQLGPTHR
GYVERANFVVLRSPDVPSILVETAFISNPAEERKLRDPTHQKKLAEAVMDGVKNYFESTP
PPGTWFAAEAARRSGVRLASRDDAGAAEDVAQRVGPPARDVHKVGRGESLGSIARQYGVS
VSALKSANQINSNTVRAGTTLTIPAG