Protein Info for MPMX19_05721 in Azospirillum sp. SherDot2

Annotation: Hydrogenase maturation factor HypB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 TIGR00073: hydrogenase accessory protein HypB" amino acids 123 to 335 (213 residues), 288.8 bits, see alignment E=1.2e-90 PF02492: cobW" amino acids 152 to 316 (165 residues), 83 bits, see alignment E=1.1e-27

Best Hits

KEGG orthology group: K04652, hydrogenase nickel incorporation protein HypB (inferred from 87% identity to azl:AZL_d02040)

Predicted SEED Role

"[NiFe] hydrogenase nickel incorporation-associated protein HypB" in subsystem NiFe hydrogenase maturation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (350 amino acids)

>MPMX19_05721 Hydrogenase maturation factor HypB (Azospirillum sp. SherDot2)
MCTVCGCGEGETRIDGKATDAGHEHVGPDGKIYRHHHGEAHHHAHHHHDDTHEHVAPDGT
VFRHSHNDHGHDHEHGHGHGHDHSHVHDHSHDHGHHHHGHDHHRPDRPDAIDGGAALNQP
RLVTIERDILAKNDGLAAVNRRRFADRGVFVLNLMSSPGSGKTTLLTRSLTDLKGRFPVA
VIEGDQQTSFDADRIRATGTPAVQVNTGKGCHLDASMVAQAADKLAADGQFAGDGVLFVE
NVGNLVCPAGFDLGETHRVVVLSVTEGEDKPLKYPDMFVRADLLVVNKIDLLPHLTFDVE
RMIGYAQRLNPSLAVIQLSATTGQGLEEWYDWIADGLAEARASHTATAAV